Human and Mouse Cell Isolation and Phenotyping

人类和小鼠细胞分离和表型分析

• 批准号: 8391963
• 负责人: Andrew S Weyrich
• 金额: $ 53.22万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-05-01 至 2017-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8391963
• 关键词: Biological Assay; Blood; Blood Clot; Blood Platelets; Blood coagulation; Breeding; Cell Separation; Cells; Center for Translational Science Activities; Diabetes Mellitus; Ensure; Equipment; Generations; Glucose; Housing; Human; In Vitro; Incubators; Instruction; Lipids; Maintenance; Medicine; Megakaryocytes; Metabolic; Metabolic syndrome; Molecular Medicine; Mus; Non-Insulin-Dependent Diabetes Mellitus; Obesity; Patients; Phenotype; Platelet Function Tests; Population; Research Personnel; Research Project Grants; Resources; Risk; Services; Sterility; Thrombosis; Tissues; Universities; Utah; Work; comparative; diabetes management; in vivo; insight; mouse model; programs

PROJECT SUMMARY (See instructions): The Human and Mouse Cell Isolation and Phenotyping Core (Core C) will provide services for all four research projects in the University of Utah Molecular Medicine Translational Research Center in Thrombosis (U2M2-TRCT) program. Core C is centrally located and immediately available to all U2M2-TRCT investigators. The core will isolate human and mouse megakaryocytes and platelets for U2M2-TRCT investigators, perform standard in vitro and in vivo platelet function assays, assist with generation, breeding, and maintenance of genetically manipulated mice proposed in projects 1 and 2, and manage mouse models of obesity and diabetes. Core C is led by a group of investigators with strengths and expertise in all of the essential core activities. In order to standardize populations of megakaryocytes and platelets among studies, Core C will isolate and culture megakaryocytes and platelets for all U2M2-TRCT investigators. In this regard. Core C will communicate directly with Core B to ensure seamless isolation of platelets from patients with obesity and diabetes. The Core will also directly communicate with projects 1 and 2 for cell isolation, metabolic phenotyping, and related functional analyses. The Core is equipped with a cell sorter and sterile incubators for isolation and culture of purified human and mouse megakaryocytes and platelets. In addition, the core has equipment for in vitro and in vivo analyses of platelet function and metabolic phenotyping of mice that will be used in projects 1 and 2. Core investigators will also work together to generate, breed, and maintain colonies of mice that are proposed in projects 1 and 2. The U2M2-TRCT program will use the expertise and resources provided by Core C to guide and direct studies proposed in projects 1-4.

项目总结（见说明）： 人类和小鼠细胞分离和表型分析核心（核心C）将为犹他州大学血栓形成分子医学转化研究中心（U2 M2-TRCT）项目的所有四个研究项目提供服务。核心C位于中心位置，所有U2 M2-TRCT研究人员都可以立即使用。核心将分离人和小鼠巨核细胞和血小板用于U2 M2-TRCT 研究人员，进行标准的体外和体内血小板功能测定，协助项目1和2中提出的遗传操作小鼠的产生、繁殖和维持，并管理肥胖和糖尿病小鼠模型。核心C由一组在所有基本核心活动方面具有优势和专业知识的调查人员领导。为了标准化研究中的巨核细胞和血小板群体，核心C将为所有U2 M2-TRCT研究者分离和培养巨核细胞和血小板。在这方面核心C将直接与核心B沟通，以确保从肥胖和糖尿病患者中无缝分离血小板。核心还将直接与项目1和2进行细胞分离，代谢表型分析和相关功能分析。核心配备了细胞分选仪和无菌 用于分离和培养纯化的人和小鼠巨核细胞和血小板的培养箱。此外，该中心还配备了用于体外和体内分析血小板功能和小鼠代谢表型的设备，这些设备将用于项目1和2。核心研究人员还将共同努力产生、繁殖和维持项目1和2中提出的小鼠群。U2 M2-TRCT计划将使用 核心C提供的专门知识和资源，以指导和指导项目1-4中提议的研究。