Mechanisms of Blood Clot Adhesion and the Design of New Wet Adhesives

血凝块粘附机制及新型湿粘合剂的设计

• 批准号: RGPIN-2018-04918
• 负责人: Kastrup, Christian
• 金额: $ 6.7万
• 依托单位: University of British Columbia
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2022
• 资助国家: 加拿大
• 起止时间: 2022-01-01 至 2023-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=760915
• 关键词: Mechanisms; Blood; Clot; Adhesion; Design

Blood clots are extraordinary adhesives. They adhere to surfaces underwater and under flow with a high degree of control to rapidly and effectively seal wounds. Although the cohesive properties of blood clots have been well studied, their adhesive properties have not. To this end, my research team was the first to report the adhesive strength of whole blood clots to blood vessel surfaces. Understanding how blood clots adhere to surfaces under harsh conditions will uncover new approaches to designing smart adhesives and sealants. Designing effective wet sealants is an important engineering goal, with a wide range of possible applications including in the food industry, and for creating clothing that protects from chemical and biological hazards.Our overarching goal is to elucidate what modulates blood clot adhesion and use this knowledge to design novel adhesives and sealants.Over the next five years, we aim to:1) Uncover the fundamental mechanisms of blood clot adhesion.We will work with human and animal blood to uncover the components of blood and surfaces that contribute to adhesion and allow clots to rapidly, controllably and effectively seal wounds. Specifically, we will explore how adhesion is influenced by self-propagation of blood clots, and incorporate this unique feature into our smart adhesives and sealants.2) Purify and optimize the components of adhesion for new applications.Once we have identified the key components of clot adhesion to natural and artificial surfaces, we will isolate and purify them and explore ways to increase their utility in industrial applications. This includes extending shelf life and expanding the range of temperatures and conditions in which adhesion can occur.3) Design new adhesives and sealant technologies.We will use the optimized clot adhesion components to engineer adhesives for specific industrial applications. This includes improving commercially available animal or bacteria-derived food glues,' which bind meat and produce together, improve texture, and reduce production costs. Another application is to design protective combat uniforms that can self-repair rips by releasing adhesives when damaged. There is military and industrial demand for self-sealing clothing that protects operators from chemical and biological hazards while being flexible, lightweight and cost-effective.My research team has a successful track record of patenting and commercializing new technologies, as well as expertise in coagulation and blood clot adhesion and engineering. Insights gained will have a major impact to the field of sealants and adhesives, blood coagulation, and to the food and defence industries. By extending our current collaborations with Defence Research and Development Canada, the program will provide interdisciplinary training to Biomedical Engineering graduate students in knowledge transfer to industry and government.

血凝块是非凡的粘合剂。它们粘附在水下和水流下的表面上，具有高度的控制力，可以快速有效地密封伤口。虽然血凝块的内聚特性已经得到了很好的研究，但它们的粘附特性还没有。为此，我的研究小组是第一个报告全血凝块与血管表面的粘附强度的人。了解血凝块在恶劣条件下如何粘附在表面上将揭示设计智能粘合剂和密封剂的新方法。设计有效的湿密封剂是一个重要的工程目标，具有广泛的可能应用，包括食品工业，以及制造防止化学和生物危害的服装。我们的首要目标是阐明调节血液凝块粘附的因素，并利用这些知识设计新型粘合剂和密封剂。在未来五年，我们的目标是：1）揭示血块粘附的基本机制。我们将与人类和动物的血液合作，以揭示血液和表面的成分，有助于粘附，并允许血块迅速，可控且有效地封闭伤口。具体而言，我们将探索血栓的自我繁殖如何影响粘附力，并将这一独特功能融入我们的智能粘合剂和密封剂中。2）纯化和优化新应用中的粘附成分一旦我们确定了凝块粘附到自然和人工表面的关键成分，我们将分离和纯化它们，并探索提高其在工业应用中的实用性的方法。这包括延长保质期和扩大粘合发生的温度和条件范围。3）设计新的粘合剂和密封剂技术。我们将使用优化的凝块粘合组分来设计用于特定工业应用的粘合剂。这包括改进市售的动物或细菌来源的食品胶，将肉类和农产品粘合在一起，改善质地，降低生产成本。另一个应用是设计保护性战斗制服，当损坏时可以通过释放粘合剂来自我修复裂缝。军事和工业对自密封服装的需求，保护操作人员免受化学和生物危害，同时具有灵活性，重量轻和成本效益。我的研究团队拥有成功的专利和商业化新技术的记录，以及凝血和血块粘附和工程方面的专业知识。所获得的见解将对密封剂和粘合剂、血液凝固以及食品和国防工业领域产生重大影响。通过扩展我们目前与加拿大国防研究与发展的合作，该计划将为生物医学工程研究生提供跨学科的培训，将知识转移到行业和政府。