Retrotransposition in Health and Disease

健康与疾病中的逆转录转座

• 批准号: 8296918
• 负责人: HAIG H. KAZAZIAN
• 金额: $ 47.73万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-05-01 至 2016-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8296918
• 关键词: Adult; Affect; Autopsy; Biochemical; Bioinformatics; Biological Assay; Biology; Brain; Bruck-de Lange syndrome; Cancer Etiology; Cell Culture Techniques; Chromatin; Collaborations; Communities; Computer Analysis; Computing Methodologies; DNA; Data; Disease; Embryonic Development; Etiology; Event; Frequencies; Gene Expression; Gene Expression Profile; Gene Frequency; Gene Targeting; Generations; Genes; Genetic Heterogeneity; Genetic Polymorphism; Genome; Genomics; Germ Lines; Health; Heart; Hemophilia A; Human; Human Biology; Human Genome; Individual; Learning; Limb structure; Liver; Location; Malignant Neoplasms; Methods; Molecular Conformation; Mosaicism; Mouse Strains; Mus; Mutation; Neuroblastoma; Normal tissue morphology; Organism; Patients; Phenotype; Placenta; Positioning Attribute; Procedures; Process; Pseudogenes; Retrotransposition; Retrotransposon; Role; Sampling; Somatic Mutation; Tail; Techniques; Testis Brain; Time; Tissues; Transgenic Animals; Variant; Work; fetal; human disease; innovation; mammalian genome; mouse genome; transposon/insertion element

DESCRIPTION (provided by applicant): The scientific community is still surprised by the mobile DNA found in the genomes of all organisms. In human beings, the master mobile DNA is the L1 retrotransposon which mobilizes itself along with Alu , SVA, and processed pseudogenes to new genomic locations. Since we found the first disease-causing insertions of an L1 in two patients with hemophilia A some 23 years ago, much has been learned about L1 and other human retrotransposons through cell culture assays, biochemical analyses, natural insertions into genes, transgenic animals, and bioinformatic analysis. Recently, PCR techniques, high-throughput sequencing and bioinformatic analysis have made it possible to discover essentially all non-reference retrotransposons in any human genome. A recent surprise finding of our lab was that in transgenic animals L1 retrotransposition (Rtn) predominately occurs in early embryonic development as opposed to the germ line. In this proposal, we propose three complementary Specific Aims that build on these findings. First, we study L1 Rtn in human biology by quantifying the somatic Rtn frequency in fetal and adult tissues and compare these frequencies to the frequency estimated for the germ line. We also determine the effects of new L1 insertions on the expression of nearby genes. Second, we have recently modified our technique to find essentially all non-reference, active mouse L1s. We use this procedure to study L1 Rtn in tissues of different mouse strains. Then, after direct determination of the germ line Rtn frequency in different mouse strains, we will compare the somatic and germ line frequencies. We expect that since the mouse has 15-30 times the number of active L1s as the human (1500-3000 vs. 80- 100), Rtn frequency in the mouse will be significantly greater than in humans. Early work has led to exciting new data, indicating that L1 insertions into the mouse genome occur non-randomly in clusters. As in the human work, we will then determine the effect of new mouse L1 insertions on gene expression. Third, we determine the role of Rtn in human disease, studying 1) various cancers whose DNA has been completely sequenced and 2) mutation-negative patients with a well-phenotyped Mendelian disorder, Cornelia de Lange Syndrome. In observing Rtn events in these patients, we will learn the extent to which they have been underestimated in human disease etiology. We predict that retrotransposon effects on human health are significantly greater than currently believed. PUBLIC HEALTH RELEVANCE: L1 retrotransposons are the major mobile DNA of human and mammalian genomes. Here we study the effects of L1 in human health and disease. We search for somatic mutations in human and mice, determine the effects of retrotransposons on gene expression in humans and mice, and analyze both human cancers and Mendelian disorders for causative retrotransposition events.

描述（由申请人提供）： 科学界仍然对在所有生物的基因组中发现的移动的DNA感到惊讶。在人类中，主要的移动的DNA是L1反转录转座子，它沿着Alu、SVA和加工过的假基因移动到新的基因组位置。自从23年前我们在两名血友病A患者中发现了第一个致病的L1插入以来，通过细胞培养试验、生化分析、基因的自然插入、转基因动物和生物信息学分析，人们对L1和其他人类逆转录转座子有了很多了解。最近，PCR技术、高通量测序和生物信息学分析使得发现任何人类基因组中基本上所有非参考反转录转座子成为可能。我们实验室最近的一个令人惊讶的发现是，在转基因动物中，L1反转录转座（Rtn）主要发生在早期胚胎发育中，而不是种系。在本提案中，我们提出了三个互补的具体目标，建立在这些发现的基础上。首先，我们研究L1 Rtn在人类生物学中的胎儿和成人组织中的体细胞Rtn频率进行量化，并将这些频率与估计的生殖系频率进行比较。我们还确定了新的L1插入对附近基因表达的影响。其次，我们最近修改了我们的技术，基本上可以找到所有非参考的活跃小鼠L1。我们使用这个程序来研究L1 Rtn在不同小鼠品系的组织中。然后，在直接测定不同小鼠品系的生殖系Rtn频率后，我们将比较体细胞和生殖系频率。我们预计，由于小鼠的活性L1数量是人类的15-30倍（1500-3000 vs. 80- 100），小鼠中的Rtn频率将显著高于人类。早期的工作已经产生了令人兴奋的新数据，表明L1插入小鼠基因组中是非随机发生的。在人类的工作中，我们将确定新的小鼠L1插入对基因表达的影响。第三，我们确定Rtn在人类疾病中的作用，研究1）DNA已完全测序的各种癌症和2）具有良好表型的孟德尔疾病科尔内利亚德兰格综合征的突变阴性患者。通过观察这些患者的Rtn事件，我们将了解它们在人类疾病病因学中被低估的程度。我们预测反转录转座子对人类健康的影响比目前认为的要大得多。 公共卫生关系： L1反转录转座子是人类和哺乳动物基因组中主要的移动的DNA。在这里，我们研究L1在人类健康和疾病中的作用。我们在人类和小鼠中寻找体细胞突变，确定反转录转座子对人类和小鼠基因表达的影响，并分析人类癌症和孟德尔疾病的致病性反转录转座事件。