Retrotransposition in Health and Disease

健康与疾病中的逆转录转座

• 批准号: 8296918
• 负责人: HAIG H. KAZAZIAN
• 金额: $ 47.73万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-05-01 至 2016-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8296918
• 关键词: Adult; Affect; Autopsy; Biochemical; Bioinformatics; Biological Assay; Biology; Brain; Bruck-de Lange syndrome; Cancer Etiology; Cell Culture Techniques; Chromatin; Collaborations; Communities; Computer Analysis; Computing Methodologies; DNA; Data; Disease; Embryonic Development; Etiology; Event; Frequencies; Gene Expression; Gene Expression Profile; Gene Frequency; Gene Targeting; Generations; Genes; Genetic Heterogeneity; Genetic Polymorphism; Genome; Genomics; Germ Lines; Health; Heart; Hemophilia A; Human; Human Biology; Human Genome; Individual; Learning; Limb structure; Liver; Location; Malignant Neoplasms; Methods; Molecular Conformation; Mosaicism; Mouse Strains; Mus; Mutation; Neuroblastoma; Normal tissue morphology; Organism; Patients; Phenotype; Placenta; Positioning Attribute; Procedures; Process; Pseudogenes; Retrotransposition; Retrotransposon; Role; Sampling; Somatic Mutation; Tail; Techniques; Testis Brain; Time; Tissues; Transgenic Animals; Variant; Work; fetal; human disease; innovation; mammalian genome; mouse genome; transposon/insertion element

DESCRIPTION (provided by applicant): The scientific community is still surprised by the mobile DNA found in the genomes of all organisms. In human beings, the master mobile DNA is the L1 retrotransposon which mobilizes itself along with Alu , SVA, and processed pseudogenes to new genomic locations. Since we found the first disease-causing insertions of an L1 in two patients with hemophilia A some 23 years ago, much has been learned about L1 and other human retrotransposons through cell culture assays, biochemical analyses, natural insertions into genes, transgenic animals, and bioinformatic analysis. Recently, PCR techniques, high-throughput sequencing and bioinformatic analysis have made it possible to discover essentially all non-reference retrotransposons in any human genome. A recent surprise finding of our lab was that in transgenic animals L1 retrotransposition (Rtn) predominately occurs in early embryonic development as opposed to the germ line. In this proposal, we propose three complementary Specific Aims that build on these findings. First, we study L1 Rtn in human biology by quantifying the somatic Rtn frequency in fetal and adult tissues and compare these frequencies to the frequency estimated for the germ line. We also determine the effects of new L1 insertions on the expression of nearby genes. Second, we have recently modified our technique to find essentially all non-reference, active mouse L1s. We use this procedure to study L1 Rtn in tissues of different mouse strains. Then, after direct determination of the germ line Rtn frequency in different mouse strains, we will compare the somatic and germ line frequencies. We expect that since the mouse has 15-30 times the number of active L1s as the human (1500-3000 vs. 80- 100), Rtn frequency in the mouse will be significantly greater than in humans. Early work has led to exciting new data, indicating that L1 insertions into the mouse genome occur non-randomly in clusters. As in the human work, we will then determine the effect of new mouse L1 insertions on gene expression. Third, we determine the role of Rtn in human disease, studying 1) various cancers whose DNA has been completely sequenced and 2) mutation-negative patients with a well-phenotyped Mendelian disorder, Cornelia de Lange Syndrome. In observing Rtn events in these patients, we will learn the extent to which they have been underestimated in human disease etiology. We predict that retrotransposon effects on human health are significantly greater than currently believed. PUBLIC HEALTH RELEVANCE: L1 retrotransposons are the major mobile DNA of human and mammalian genomes. Here we study the effects of L1 in human health and disease. We search for somatic mutations in human and mice, determine the effects of retrotransposons on gene expression in humans and mice, and analyze both human cancers and Mendelian disorders for causative retrotransposition events.

描述（由申请人提供）：