Extracellular matrix and the function and stability of FoxP3+ regulatory T-cells

描述(申请人提供)：受伤和愈合的胰岛中的炎症环境是自身免疫性糖尿病发展的决定性因素，但我们对这种环境或它如何参与免疫调节知之甚少。我们最近确定了透明质酸(HA)在Foxp3+调节性T细胞(Treg)功能和稳定性中的关键作用，透明质酸(HA)是损伤和愈合组织细胞外基质(ECM)的组成部分，Foxp3+调节性T细胞(Treg)是T细胞亚群 对维持免疫耐受性至关重要。HA对Treg的作用取决于其大小。高分子量HA(HMW-HA)存在于愈合组织中，可促进Treg的存活和功能。HMW-HA通过交联CD44并在Treg所需的IL-2R信号中替代IL-2来实现这一点，这是其生存所需的，并产生关键的免疫调节细胞因子IL-10。与此一致的是，CD44-/-小鼠对损伤的反应是持续的炎症，Treg较少，IL-10的产生也较少。因此，HMW-HA起着组织完整性信号的作用，支持愈合组织中的Treg。低分子量透明质酸(LMW-HA)是慢性炎症组织的特征，它抑制Treg功能并抑制IL-10的产生。LMW-HA是一种Toll样受体2(TLR2)配体；LMW-HA可以负向调节无菌炎症部位的Treg功能，就像微生物TLR2配体在活跃感染部位抑制Treg一样。总之，我们的数据支持一种模型，即HA的分解代谢和通过CD44和TLR2的信号将炎症与无菌损伤部位的Treg功能联系起来。如果这个模型是正确的，就有可能通过支持HA的完整性来增强Treg功能、促进伤口愈合和防止自身免疫。在体内，透明质酸的完整性由TSG-6和其他透明质酸结合分子(透明粘附素)共同连接透明质酸链来维持。这防止了它们的降解，并促进了与CD44的相互作用。TSG-6已被实验用于治疗脓毒症和其他形式的炎症，但其在自身免疫中的价值尚不清楚。在这里，我们将阐明HMW-HA和LMW-HA控制Treg行为的机制，并通过自身免疫性糖尿病小鼠模型确定HA完整性在体内Treg功能中所起的作用。这些研究的预期结果是掌握管理受伤和愈合组织中免疫调节的机制的工作知识，以及促进伤口愈合和防止自身免疫的创新的基于ECM的策略。 公共卫生相关性：拟议的研究将极大地促进我们对受损和愈合胰岛的免疫调节机制的理解，并将为预防自身免疫性糖尿病的创新策略打开大门。

DESCRIPTION (provided by applicant): The inflammatory milieu in injured and healing islets is a decisive factor in the development of autoimmune diabetes and yet we know very little about this milieu or how it contributes to immune regulation. We have recently identified a pivotal role for hyaluronan (HA), a component of the extracellular matrix (ECM) of injured and healing tissues, in the function and stability of Foxp3+ regulatory T-cells (Treg), a T-cell subset critical to the maintenance of immune tolerance. The effect of HA on Treg depends on its size. High molecular weight HA (HMW-HA), found in healing tissues, promotes Treg survival and function. HMW-HA does this by crosslinking CD44 and substituting for IL-2 in the IL-2R signaling required by Treg for their viability and for production of the key immunoregulatory cytokine IL-10. Consistent with this, CD44-/- mice have unremitting inflammation in response to injury, fewer Treg, and make less IL-10. HMW-HA therefore functions as a tissue integrity signal that supports Treg in healing tissues. Low-molecular weight HA (LMW-HA), characteristic of chronically inflamed tissues, inhibits Treg function and suppresses production of IL-10. LMW-HA is a Toll like receptor 2 (TLR2) ligand; LMW-HA may negatively regulate Treg function at sites of sterile inflammation in the same way that microbial TLR2 ligands are known to inhibit Treg at sites of active infection. Together, our data support a model whereby HA catabolism and signaling through CD44 and TLR2 link inflammation to Treg function at sites of sterile injury. If this model is correct, it may be possible to enhance Treg function, promote wound healing and prevent autoimmunity by supporting HA integrity. In vivo HA integrity is maintained by TSG-6 and other HA-binding molecules (hyaladherins) that covalently link HA strands. This prevents their degradation and promotes interactions with CD44. TSG-6 has been used experimentally to treat sepsis and other forms of inflammation but its value in autoimmunity is unknown. Here, we will elucidate the mechanisms by which HMW-HA and LMW-HA govern Treg behavior and determine what role HA integrity plays in Treg function in vivo using mouse models of autoimmune diabetes. The expected outcomes of these studies are a working knowledge of the mechanisms that govern immune regulation in injured and healing tissues and innovative, ECM-based strategies to promote wound healing and prevent autoimmunity. PUBLIC HEALTH RELEVANCE: The proposed studies will greatly advance our understanding of the mechanisms that govern immune regulation in injured and healing pancreatic islets and will open the doors to innovative strategies to prevent autoimmune diabetes.