CALCIUM REGULATION IN ORAL HEALTH

口腔健康中的钙调节

基本信息

  • 批准号:
    8354594
  • 负责人:
  • 金额:
    $ 10.69万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2012
  • 资助国家:
    美国
  • 起止时间:
    2012-07-13 至 2014-06-30
  • 项目状态:
    已结题

项目摘要

DESCRIPTION (provided by applicant): Calcium is a key regulator of a broad range of biological functions and is also a key element in the composition of dental enamel. During the maturation stage of amelogenesis, Ca2+ requirements increase as enamel crystals expand in width and thickness. Calcium must reach the forming enamel layer but how this process is regulated in ameloblasts is poorly understood. The current model for Ca2+ transport in enamel focuses on the transcellular passage of Ca2+ via the entry, transit and extrusion steps. Whereas several works have reported on the transit and extrusion steps, only limited information is available for Ca2+ entry mechanisms into ameloblasts. In this grant proposal, I focus on the entry step by examining the role of the store-operated Ca2+ release-activated Ca2+ (CRAC) channels. CRAC channels comprise important Ca2+ influx mechanism in epithelial cells. Patients with mutations to CRAC channels (STIM1, ORAI1) present, in addition to immune system deficiencies, with hypocalcified amelogenesis imperfecta. My goal is to identify how Stim1 and Orai1 are involved in Ca2+ entry and how this process is regulated. Recent work by the PI indicates that Stim1, Orai1 as well as well as the Ca2+ signaling cholecystokinin (Cck) and the Cck inhibitor Rcan1 were identified as being significantly up-regulated in maturation. I have confirmed these results by qPCR, Western blot and IHC. Thus Ca2+ influx into ameloblasts via CRAC channels and how this process is regulated is important for the development of healthy enamel. Evidence contributed by the proposed grant application will help to better understand the systemic effects of CRAC function abrogation. This will also help medical practitioners in making decisions concerning dentist visits to patients with mutations to CRAC channels in order to prevent dental problems PUBLIC HEALTH RELEVANCE: Calcium is critical for the normal biomineralization of enamel. Disruptions to calcium uptake by ameloblasts results in amelogenesis imperfecta. My goal is to analyze the function of calcium-store operated channels in enamel development.
说明(申请人提供):钙是一系列生物功能的关键调节剂,也是牙釉质成分中的关键元素。在釉质形成的成熟阶段,随着釉质晶体的宽度和厚度的增加,对钙的需求也会增加。钙必须到达形成牙釉质的层,但这一过程在成釉细胞中是如何调节的,人们知之甚少。目前牙釉质中钙离子转运的模型主要集中在钙离子进入、转运和排出过程中的跨细胞转运。虽然已经有几项工作报道了转运和排出的步骤,但只有有限的信息可用于钙离子进入成釉细胞的机制。在这项拨款提案中,我通过研究存储操作的钙释放激活的钙通道(CRAC)的作用,将重点放在进入步骤上。CRAC通道是上皮细胞内钙内流的重要机制。CRAC通道(STIM1,ORAI1)突变的患者除了免疫系统缺陷外,还存在低钙化的釉质形成不全。我的目标是确定STIM1和Orai1是如何参与钙离子内流的,以及这个过程是如何调控的。PI最近的工作表明,STIM1、Orai1以及钙信号通路CCK和CCK抑制剂RCAN1在成熟过程中显著上调。我用定量聚合酶链式反应、免疫印迹和免疫组织化学方法证实了这些结果。因此,钙离子通过CRAC通道进入成釉细胞,如何调控这一过程对健康釉质的发育至关重要。拟议拨款申请提供的证据将有助于更好地理解取消CRAC职能的系统性影响。这也将帮助医生作出决定,牙医访问CRAC频道的突变患者的牙医,以防止牙齿问题 与公众健康相关:钙对牙釉质的正常生物矿化至关重要。成釉细胞对钙的摄取中断会导致成釉细胞发育不全。我的目标是分析钙库操作通道在釉质发育中的作用。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

Rodrigo S. Lacruz其他文献

Casup2+/sup-activated chloride channel ANO1: A new regulator of osteoclast function
  • DOI:
    10.1016/j.ceca.2022.102633
  • 发表时间:
    2022-09-01
  • 期刊:
  • 影响因子:
    4.000
  • 作者:
    Nicola C. Partridge;Rodrigo S. Lacruz
  • 通讯作者:
    Rodrigo S. Lacruz
The evolutionary history of the human face
人类面部的进化史
  • DOI:
    10.1038/s41559-019-0865-7
  • 发表时间:
    2019-04-15
  • 期刊:
  • 影响因子:
    14.500
  • 作者:
    Rodrigo S. Lacruz;Chris B. Stringer;William H. Kimbel;Bernard Wood;Katerina Harvati;Paul O’Higgins;Timothy G. Bromage;Juan-Luis Arsuaga
  • 通讯作者:
    Juan-Luis Arsuaga

Rodrigo S. Lacruz的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('Rodrigo S. Lacruz', 18)}}的其他基金

Redox and Ca2+ signaling regulation of enamel mineralization
牙釉质矿化的氧化还原和 Ca2 信号传导调节
  • 批准号:
    10586833
  • 财政年份:
    2023
  • 资助金额:
    $ 10.69万
  • 项目类别:
Molecular mechanisms of oral deficiencies in Down syndrome
唐氏综合症口腔缺陷的分子机制
  • 批准号:
    10658410
  • 财政年份:
    2023
  • 资助金额:
    $ 10.69万
  • 项目类别:
Redox and Ca2+ signaling regulation of enamel mineralization
牙釉质矿化的氧化还原和 Ca2 信号传导调节
  • 批准号:
    10162310
  • 财政年份:
    2018
  • 资助金额:
    $ 10.69万
  • 项目类别:
Calcium Control of Enamel Development
牙釉质发育的钙控制
  • 批准号:
    9124353
  • 财政年份:
    2016
  • 资助金额:
    $ 10.69万
  • 项目类别:
Calcium Control of Enamel Development
牙釉质发育的钙控制
  • 批准号:
    9493459
  • 财政年份:
    2016
  • 资助金额:
    $ 10.69万
  • 项目类别:
Calcium Regulation and Oral Health
钙调节和口腔健康
  • 批准号:
    8733843
  • 财政年份:
    2014
  • 资助金额:
    $ 10.69万
  • 项目类别:
Calcium Regulation and Oral Health
钙调节和口腔健康
  • 批准号:
    8811334
  • 财政年份:
    2014
  • 资助金额:
    $ 10.69万
  • 项目类别:
CALCIUM REGULATION IN ORAL HEALTH
口腔健康中的钙调节
  • 批准号:
    8510624
  • 财政年份:
    2012
  • 资助金额:
    $ 10.69万
  • 项目类别:

相似海外基金

RII Track-4:NSF: From the Ground Up to the Air Above Coastal Dunes: How Groundwater and Evaporation Affect the Mechanism of Wind Erosion
RII Track-4:NSF:从地面到沿海沙丘上方的空气:地下水和蒸发如何影响风蚀机制
  • 批准号:
    2327346
  • 财政年份:
    2024
  • 资助金额:
    $ 10.69万
  • 项目类别:
    Standard Grant
BRC-BIO: Establishing Astrangia poculata as a study system to understand how multi-partner symbiotic interactions affect pathogen response in cnidarians
BRC-BIO:建立 Astrangia poculata 作为研究系统,以了解多伙伴共生相互作用如何影响刺胞动物的病原体反应
  • 批准号:
    2312555
  • 财政年份:
    2024
  • 资助金额:
    $ 10.69万
  • 项目类别:
    Standard Grant
How Does Particle Material Properties Insoluble and Partially Soluble Affect Sensory Perception Of Fat based Products
不溶性和部分可溶的颗粒材料特性如何影响脂肪基产品的感官知觉
  • 批准号:
    BB/Z514391/1
  • 财政年份:
    2024
  • 资助金额:
    $ 10.69万
  • 项目类别:
    Training Grant
Graduating in Austerity: Do Welfare Cuts Affect the Career Path of University Students?
紧缩毕业:福利削减会影响大学生的职业道路吗?
  • 批准号:
    ES/Z502595/1
  • 财政年份:
    2024
  • 资助金额:
    $ 10.69万
  • 项目类别:
    Fellowship
感性個人差指標 Affect-X の構築とビスポークAIサービスの基盤確立
建立个人敏感度指数 Affect-X 并为定制人工智能服务奠定基础
  • 批准号:
    23K24936
  • 财政年份:
    2024
  • 资助金额:
    $ 10.69万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Insecure lives and the policy disconnect: How multiple insecurities affect Levelling Up and what joined-up policy can do to help
不安全的生活和政策脱节:多种不安全因素如何影响升级以及联合政策可以提供哪些帮助
  • 批准号:
    ES/Z000149/1
  • 财政年份:
    2024
  • 资助金额:
    $ 10.69万
  • 项目类别:
    Research Grant
How does metal binding affect the function of proteins targeted by a devastating pathogen of cereal crops?
金属结合如何影响谷类作物毁灭性病原体靶向的蛋白质的功能?
  • 批准号:
    2901648
  • 财政年份:
    2024
  • 资助金额:
    $ 10.69万
  • 项目类别:
    Studentship
ERI: Developing a Trust-supporting Design Framework with Affect for Human-AI Collaboration
ERI:开发一个支持信任的设计框架,影响人类与人工智能的协作
  • 批准号:
    2301846
  • 财政年份:
    2023
  • 资助金额:
    $ 10.69万
  • 项目类别:
    Standard Grant
Investigating how double-negative T cells affect anti-leukemic and GvHD-inducing activities of conventional T cells
研究双阴性 T 细胞如何影响传统 T 细胞的抗白血病和 GvHD 诱导活性
  • 批准号:
    488039
  • 财政年份:
    2023
  • 资助金额:
    $ 10.69万
  • 项目类别:
    Operating Grants
How motor impairments due to neurodegenerative diseases affect masticatory movements
神经退行性疾病引起的运动障碍如何影响咀嚼运动
  • 批准号:
    23K16076
  • 财政年份:
    2023
  • 资助金额:
    $ 10.69万
  • 项目类别:
    Grant-in-Aid for Early-Career Scientists
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了