In situ analysis of bacterial biosynthetic activity in subgingival plaque

龈下菌斑细菌生物合成活性的原位分析

• 批准号: 8270464
• 负责人: Gerard A Cangelosi
• 金额: $ 1.74万
• 依托单位: SEATTLE BIOMEDICAL RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8270464
• 关键词: Anabolism; Bacteria; Biology; Cell division; Cells; Chronic; Clinical; Communities; Complex; DNA; Diagnosis; Disease; Disease Progression; Disorder by Site; Etiology; Future; Genomics; Goals; Grant; Growth; Human; In Situ; In Vitro; Laboratories; Life; Longitudinal Studies; Measurement; Measures; Methods; Modeling; Molecular; Nutritional; Oral cavity; Patients; Periodontal Diseases; Periodontium; Phase; Physiological; Porphyromonas gingivalis; Preventive; Public Health; Quantitative Reverse Transcriptase PCR; Research; Ribosomal RNA; Risk; Sampling; Site; Staging; Testing; Time; base; disease diagnosis; human subject; improved; microbial; microorganism; novel; novel strategies; pathogen; rRNA Precursor; subgingival biofilm; tool

DESCRIPTION (provided by applicant): Periodontal diseases are induced by communities of subgingival bacteria and result in destruction of supporting structures of the teeth. The diseases are diagnosed retrospectively by clinical parameters, however these parameters are poor indicators of ongoing disease activity or future attachment loss. An important research goal is to devise a means to identify sites in patients' mouths that are at risk of periodontal disease progression, thereby allowing site-specific preventive therapy. We hypothesize that active cell division of potential pathogens such as Porphyromonas gingivalis correlates with periodontal disease progression at specific sites. To test this hypothesis, a novel PCR-based molecular approach will be used to measure, in real time, the biosynthetic and growth activity of P. gingivalis cells in human subgingival plaque samples collected from healthy and disease sites. The project will test the hypothesis that P. gingivalis growth activity is greater in disease sites than in healthy sites of human subjects. The results will set the stage for future longitudinal studies that evaluate the efficacy of this approach, targeted to P. gingivalis as well as to other periodontal microorganisms, as clinically useful predictors of periodontal disease progression. Similar approaches also could be applied to other chronic, multifactorial diseases. When applied on a microbiomic scale, these methods could also significantly improve our understanding of specific microbiological activities within multi-species consortia including subgingival biofilms.

描述（由申请人提供）：牙周病是由龈下细菌群落引起的，并导致牙齿支撑结构的破坏。这些疾病通过临床参数进行回顾性诊断，然而这些参数是正在进行的疾病活动或未来附着丧失的不良指标。一个重要的研究目标是设计一种方法来识别患者口腔中有牙周病进展风险的部位，从而允许针对特定部位的预防性治疗。我们假设潜在病原体如牙龈卟啉单胞菌的活跃细胞分裂与特定部位的牙周病进展相关。为了检验这一假设，将使用一种新型的基于PCR的分子方法来真实的测量从健康和疾病部位收集的人类牙龈下菌斑样本中牙龈卟啉单胞菌细胞的生物合成和生长活性。该项目将检验牙龈卟啉单胞菌在人类受试者的患病部位比健康部位生长活性更大的假设。这些结果将为未来的纵向研究奠定基础，这些研究将评估这种方法的有效性，针对牙龈卟啉单胞菌以及其他牙周微生物，作为牙周病进展的临床有用的预测因子。类似的方法也可以应用于其他慢性多因素疾病。当应用于微生物组学规模时，这些方法也可以显着提高我们对多物种聚生体（包括龈下生物膜）内特定微生物活性的理解。