Oral Colonization of Aggregatibacter in Primates
灵长类动物中聚集杆菌的口腔定植
基本信息
- 批准号:8709162
- 负责人:
- 金额:$ 15.3万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2011
- 资助国家:美国
- 起止时间:2011-04-01 至 2015-02-28
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
DESCRIPTION (provided by applicant): Aggregatibacter actinomycetemcomitans (Aa) is intimately associated with Localized Aggressive Periodontitis (LAP) in young adults. Further, Aa possesses a variety of virulence traits that are consistent with pathogenic events that occur in LAP. Our group has been studying genes that are related to Aa-induced disease initiation and as such encode virulence traits responsible for Aa attachment, colonization, persistence, and subgingival survival. Thus far we have ascribed functions for two Aa autotransporter adhesin genes (aae and apiA) that are related to the specificity of Aa attachment to epithelium and have shown that these adhesins (as well as Leukotoxin) show species specificity for Old World (OW) primates and humans. This specificity makes the OW primate an ideal model for studying early events related to Aa infection with an eye toward development of preventive strategies. This R21 application consists of two Aims and is designed to compare oral colonization and persistence of two Aa strains (one from humans and one from Rhesus [Rh] monkeys) that are introduced into the oral cavity of OW primates. Each strain will be examined for its pattern, level of attachment, and colonization at different times over a 28-day period following introduction into the mouth of the monkey. Aim 1 will describe the topographical and quantitative level of Aa found in the mouths of Rh monkeys initially and then compare colonization and persistence of the two strains of Aa (one human and one monkey). Before placement, monkeys will receive scaling and prophylaxis plus chlorhexidine treatment. Animals will be fed Aa in a pancake and colonization and persistence will be analyzed 28 days after feeding. The Aa that colonizes and persists on teeth at a level equal to or greater than 1 x 102 /mL colonies of Aa will be selected for use in Aim 2. Aim 2 will assess the location, level and timing of Aa found on BECs, tongue and teeth comparing un-inoculated and wild type inoculated Aa to Aa with mutations in aae and apiA, a double knockout of aae/apiA, flp (the fibrillar outer protein) and ltx over a 28-day period. These experiments should reveal the importance of these genes in relation to Aa attachment to BECs (aae and apiA), to tooth colonization (flp) and to subgingival survival (ltx) in the oral cavity of OW primates. Establishing the utility of this model should allow us not only to dissect out attachment factors but also to unravel immune modulation factors from other Aa genes as they affect Aa pathogenesis in the future with an eye toward preventive strategies.
描述(由申请人提供):放线菌聚合杆菌(Aa)与年轻人的局部侵袭性牙周炎(LAP)密切相关。此外,Aa具有多种毒力性状,与LAP中发生的致病事件一致。我们的团队一直在研究与Aa诱导的疾病起始相关的基因,并以此编码负责Aa附着、定植、持久性和龈下存活的毒力特征。到目前为止,我们已经将两个Aa自转运体黏附素基因(aae和apiA)的功能与Aa附着于上皮的特异性相关,并表明这些黏附素(以及白质毒素)在旧大陆(OW)灵长类动物和人类中具有物种特异性。这种特异性使得OW灵长类动物成为研究与Aa感染相关的早期事件并着眼于制定预防策略的理想模型。本R21应用程序由两个目的组成,旨在比较两种Aa菌株(一种来自人类,一种来自恒河猴)的口腔定植和持久性,这两种Aa菌株被引入OW灵长类动物的口腔。在将每个菌株引入猴口中后的28天内,将在不同时间检查其模式、附着水平和定植。目的1将首先描述Rh猴口腔中Aa的地形和数量水平,然后比较两种Aa菌株(一种人和一种猴)的定植和持久性。在放置之前,猴子将接受洗牙和预防以及氯己定治疗。动物将在煎饼中喂食Aa,并在喂食后28天分析定植和持久性。在牙齿上定植并持续存在的Aa等于或大于1 × 102 /mL菌落的Aa将被选择用于Aim 2。目的2将评估BECs、舌头和牙齿上发现的Aa的位置、水平和时间,比较未接种和野生型接种的Aa与aae和apiA突变的Aa, aae/apiA、flp(纤原外蛋白)和ltx在28天内的双敲除。这些实验将揭示这些基因在Aa附着于BECs (aae和apiA)、牙齿定植(flp)和OW灵长类动物口腔龈下存活(ltx)方面的重要性。建立该模型的实用性,不仅可以让我们剖析附着因子,还可以揭示其他Aa基因的免疫调节因子,因为它们影响Aa的发病机制,并着眼于预防策略。
项目成果
期刊论文数量(4)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Colonization and Persistence of Labeled and "Foreign" Strains of Aggregatibacter actinomycetemcomitans Inoculated into the Mouths of Rhesus Monkeys.
- DOI:10.13188/2377-987x.1000005
- 发表时间:2015-01-01
- 期刊:
- 影响因子:0
- 作者:Fine, Daniel H;Karched, Maribasappa;Godboley, Dipti
- 通讯作者:Godboley, Dipti
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
DANIEL H FINE其他文献
DANIEL H FINE的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('DANIEL H FINE', 18)}}的其他基金
Oral Colonization of Aggregatibacter in Primates
灵长类动物中聚集杆菌的口腔定植
- 批准号:
8107051 - 财政年份:2011
- 资助金额:
$ 15.3万 - 项目类别:
Oral Colonization of Aggregatibacter in Primates
灵长类动物中聚集杆菌的口腔定植
- 批准号:
8249833 - 财政年份:2011
- 资助金额:
$ 15.3万 - 项目类别:
ORAL DISTRIBUTION OF AGGREGATIBACTER ACTINOMYCETEMCOMITANS IN OLD WORLD MONKEYS
旧世界猴群中放线菌共生菌的口腔分布
- 批准号:
8358013 - 财政年份:2011
- 资助金额:
$ 15.3万 - 项目类别:
Localized Aggressive Periodontitis: Microbial & Host Markers for Susceptibility
局部侵袭性牙周炎:微生物
- 批准号:
7932560 - 财政年份:2009
- 资助金额:
$ 15.3万 - 项目类别:
Localized Aggressive Periodontitis: Microbial & Host Markers for Susceptibility
局部侵袭性牙周炎:微生物
- 批准号:
7871397 - 财政年份:2007
- 资助金额:
$ 15.3万 - 项目类别:
Localized Aggressive Periodontitis: Microbial & Host Markers for Susceptibility
局部侵袭性牙周炎:微生物
- 批准号:
7468418 - 财政年份:2007
- 资助金额:
$ 15.3万 - 项目类别:
Localized Aggressive Periodontitis: Microbial & Host Markers for Susceptibility
局部侵袭性牙周炎:微生物
- 批准号:
8092569 - 财政年份:2007
- 资助金额:
$ 15.3万 - 项目类别:
Localized Aggressive Periodontitis: Microbial & Host Markers for Susceptibility
局部侵袭性牙周炎:微生物
- 批准号:
7644510 - 财政年份:2007
- 资助金额:
$ 15.3万 - 项目类别:
Localized Aggressive Periodontitis: Microbial & Host Markers for Susceptibility
局部侵袭性牙周炎:微生物
- 批准号:
7321527 - 财政年份:2007
- 资助金额:
$ 15.3万 - 项目类别:
相似海外基金
CAREER: Quaternary paleontology of Jamaica: Colonization, extinction, and resilience in a biodiversity hotspot
职业:牙买加第四纪古生物学:生物多样性热点地区的殖民、灭绝和复原力
- 批准号:
2340910 - 财政年份:2024
- 资助金额:
$ 15.3万 - 项目类别:
Continuing Grant
Determining the dynamics of the colonization of the pelagic realm and evolution of the early Palaeozoic biological pump
确定远洋领域殖民的动态和早古生代生物泵的演化
- 批准号:
NE/X017745/1 - 财政年份:2024
- 资助金额:
$ 15.3万 - 项目类别:
Fellowship
CAREER: Enhancing probiotic yeast colonization for stable in situ biomanufacturing
职业:增强益生菌酵母定殖以实现稳定的原位生物制造
- 批准号:
2239428 - 财政年份:2023
- 资助金额:
$ 15.3万 - 项目类别:
Continuing Grant
Exploration of factors promoting colonization in transplanted enteric nerves and their potential clinical application in the treatment of Hirschsprung's disease
移植肠神经定植促进因子的探索及其在先天性巨结肠治疗中的潜在临床应用
- 批准号:
23K08061 - 财政年份:2023
- 资助金额:
$ 15.3万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Mechanisms of Candida auris Colonization in the Skin
耳念珠菌在皮肤中定植的机制
- 批准号:
10717197 - 财政年份:2023
- 资助金额:
$ 15.3万 - 项目类别:
Metabolic determinants of Staphylococcus aureus skin colonization
金黄色葡萄球菌皮肤定植的代谢决定因素
- 批准号:
10749745 - 财政年份:2023
- 资助金额:
$ 15.3万 - 项目类别:
EAGER: Colonization at the Fringe of Habitability
渴望:在宜居性边缘的殖民
- 批准号:
2305593 - 财政年份:2023
- 资助金额:
$ 15.3万 - 项目类别:
Standard Grant
Mechanisms of Klebsiella pneumoniae gastrointestinal colonization
肺炎克雷伯菌胃肠道定植机制
- 批准号:
10736879 - 财政年份:2023
- 资助金额:
$ 15.3万 - 项目类别:
Biasing immunological development with early life microbial colonization
生命早期微生物定植导致免疫发育偏向
- 批准号:
10730933 - 财政年份:2023
- 资助金额:
$ 15.3万 - 项目类别:
Molecular detection of intrauterine microbial colonization in women with adenomyosis and occurrence of chronic endometritis
子宫腺肌病女性宫内微生物定植及慢性子宫内膜炎发生的分子检测
- 批准号:
23K08893 - 财政年份:2023
- 资助金额:
$ 15.3万 - 项目类别:
Grant-in-Aid for Scientific Research (C)