ORAL DISTRIBUTION OF AGGREGATIBACTER ACTINOMYCETEMCOMITANS IN OLD WORLD MONKEYS

该子项目是利用资源的许多研究子项目之一 由NIH/NCRR资助的中心赠款提供。次级项目的主要支助 子项目的主要研究者可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 伴放线菌聚集杆菌（Aggregatibacteractinomycetemcomitans，Aa）是一种革兰氏阴性的嗜二氧化碳口腔细菌，被认为是引起青春期前和青少年局部侵袭性牙周炎（LocalizedAggregressivePeriodontitis，OAP）的致病菌。 它在体外已被证明专属于人类和旧世界猴的颊上皮细胞。 这种附着是由细菌表面蛋白aae和apiA介导的，它们是附着到颊上皮细胞膜上的配体的受体。 此外，口腔上皮似乎是人类中Aa的主要储存库。 本研究的目的是确定Aa在灵长类动物口腔中最早的口腔定殖模式，并确定apiA和aae在定殖模式中的作用。 用口腔拭子和牙菌斑刮片筛选猴是否存在Aa细菌，并用新型PCR测定法鉴定细菌菌株。 此外，在用防腐剂溶液对口腔进行消毒后，用Aa细菌接种一小组猴子。 包括基因敲除和抗生素抗性菌株在内的各种菌株的接种将有助于确定Aa细菌的宿主范围和人类疾病的非人灵长类动物模型的使用。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Aggregatibacter actinomycetemcomitans (Aa) is a gram negative capnophilic oral bacteria and the putative cause of Localized Aggressive Periodontitis (LAP) in preteens and teenagers. It has been shown in vitro to colonize buccal epithelial cells of humans and old world monkeys exclusively. This attachment is mediated by bacterial surface proteins, aae and apiA which are receptors which attach to ligands on the buccal epithelial cell membrane. Further, the buccal epithelium appears to be a primary reservoir of Aa in humans. The purpose of this study is to determine the earliest patterns of oral colonization of Aa in the primate mouth and determine the roles of apiA and aae in the pattern of colonization. Monkeys are screened with oral swabs and dental plaque scraping for presence of Aa bacteria and the strain of bacteria identified with novel PCR assays. Additionally, a smaller group of monkeys is inoculated with Aa bacteria following disinfection of the oral cavity with antiseptic solution. Inoculation of various strains including knockout and antibiotic resistant strains will help to determine the host range for Aa bacteria and the use of a nonhuman primate model fo the human disease.