ORAL DISTRIBUTION OF AGGREGATIBACTER ACTINOMYCETEMCOMITANS IN OLD WORLD MONKEYS

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 Aggregatibacter放线菌(Aggregatibacter Actinmycetcomcomitans，AA)是一种革兰氏阴性嗜酸口腔细菌，是儿童和青少年局限性侵袭性牙周炎(LAP)的病原菌。它已被证明在体外只在人类和旧大陆猴子的口腔上皮细胞中定植。这种附着是由细菌表面蛋白AAE和ApIA介导的，它们是附着在口腔上皮细胞膜上的配体上的受体。此外，口腔上皮似乎是人类再生障碍性贫血的主要储存库。本研究的目的是确定AA在灵长类动物口腔中最早的定植模式，并确定Apia和AAE在定植模式中的作用。 用口腔拭子和牙菌斑刮片对猴子进行筛查，以确定AA细菌的存在和用新的PCR分析鉴定的细菌菌株。此外，在用消毒液对口腔进行消毒后，一小部分猴子被接种AA细菌。接种各种菌株，包括基因敲除和抗药性菌株，将有助于确定AA细菌的宿主范围，并使用非人类灵长类动物模型来治疗人类疾病。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Aggregatibacter actinomycetemcomitans (Aa) is a gram negative capnophilic oral bacteria and the putative cause of Localized Aggressive Periodontitis (LAP) in preteens and teenagers. It has been shown in vitro to colonize buccal epithelial cells of humans and old world monkeys exclusively. This attachment is mediated by bacterial surface proteins, aae and apiA which are receptors which attach to ligands on the buccal epithelial cell membrane. Further, the buccal epithelium appears to be a primary reservoir of Aa in humans. The purpose of this study is to determine the earliest patterns of oral colonization of Aa in the primate mouth and determine the roles of apiA and aae in the pattern of colonization. Monkeys are screened with oral swabs and dental plaque scraping for presence of Aa bacteria and the strain of bacteria identified with novel PCR assays. Additionally, a smaller group of monkeys is inoculated with Aa bacteria following disinfection of the oral cavity with antiseptic solution. Inoculation of various strains including knockout and antibiotic resistant strains will help to determine the host range for Aa bacteria and the use of a nonhuman primate model fo the human disease.