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EXPERIMENTAL PATHOLOGY CORE

实验病理学核心

基本信息

批准号：
8052122
负责人：
GERALD C CHU
金额：
$ 8.38万
依托单位：
DANA-FARBER CANCER INST
依托单位国家：
美国
项目类别：
财政年份：
2011
资助国家：
美国
起止时间：
2011-01-01 至 2011-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8052122
关键词：
Activation Analysis Anatomy Antibodies Appearance Autopsy Biological Assay Biology Body part Cells Clinical Collaborations Development Disease Engineering Ensure Environment Evaluation Experimental Pathology Extinction (Psychology)Fluorochrome Freezing Future Gene Mutation Genetic Genetically Engineered Mouse Goals Growth Heterogeneity Histologic Histology Hour Human Image Image Analysis Imaging Techniques Immunohistochemistry In Situ Institutional Review Boards Investigation KRAS2 gene Laser Scanning Cytometry MAP Kinase Gene MEK inhibition MEKs Maintenance Malignant Neoplasms Malignant neoplasm of pancreas Metabolic Metabolic Pathway Metabolism Microscope Modeling Molecular Mus Mutation Neoplasm Metastasis Pancreas Pancreatic Ductal Adenocarcinoma Pancreatic Intraepithelial Neoplasia Paraffin Tissue Pathologic Pathway interactions Patients Pharmaceutical Preparations Pharmacological Treatment Phenotype Phospho-Specific Antibodies Phosphotransferases Quantum Dots Reagent Research Research Infrastructure Research Personnel Resources Sampling Services Signal Pathway Signal Transduction Small Interfering RNA Specimen Staining method Stains Stromal Invasion Supervision System Technical Expertise Techniques Technology Tissue Banking Tissue Banks Tissue Harvesting Tissue Microarray Tissue Sample Tissues Translating Tumor Tissue Validation Work Xenograft Model Xenograft procedure base biobank cell type experience fluorophore gene discovery genetic manipulation human tissue in vivo insight liquid crystal polymer mTOR Inhibitor member molecular imaging molecular pathology molecular/cellular imaging mouse model novel pancreatic neoplasm response subcutaneous tissue processing tool transcriptomics tumor tumor xenograft

项目摘要

This Experimental Pathology Core is an integral component of the P01 for its expertise in characterizing the morphologic, molecular and metabolic phenotype of pancreatic cancer, from inception to metastasis, in their diverse states determined by targeted genetic manipulations and study of human tumor specimens. Genetic alterations, as evaluated in mouse models, will be translated and assessed in clinical PDAC tumors for validation purposes. This Core will work in close collaboration with the Project Investigators to achieve three specific aims: 1) to provide histology services and consultative expertise including centralized review in the pathologic evaluation of mouse and human pancreatic neoplasms; ii) to provide infrastructure and technical expertise for a variety of molecular pathologic assays; and iii) to evaluate and implement new ex vivo organotypic culture assays and advanced imaging and analysis technologies that will greatly facilitate these research goals in future. These Aims encompass a wide variety of specialized techniques performed on genetically engineered mouse models, mouse xenograft models, and IRB-approved analysis of human patient tissue specimens. Services will be provided in close Integration with the BioBank Core, Imaging Core and GEM Core. Members of the Experimental Pathology Core have extensive experience in achieving both service-oriented goals as well as pioneering new molecular pathology research tools. The Core will seek to ensure a high quality of interpretive and developmental work to facilitate the goals of the P01.

该实验病理学核心是P01的一个组成部分，因为它在表征胰腺癌的形态学，分子和代谢表型方面具有专业知识，从开始到转移，通过靶向遗传操作和人类肿瘤标本的研究确定其不同状态。在小鼠模型中评价的遗传改变将在临床PDAC肿瘤中进行翻译和评估，以进行验证。该核心将与项目研究者密切合作，以实现三个具体目标：1）提供组织学服务和咨询专业知识，包括对小鼠和人类胰腺肿瘤病理学评价的集中审查; ii）为各种分子病理学检测;和iii）评估和实施新的离体器官型培养测定和先进的成像和分析技术，这将极大地促进未来的这些研究目标。这些目标包括在基因工程小鼠模型、小鼠异种移植模型和IRB批准的人类患者组织标本分析中进行的各种专业技术。服务将与BioBank Core、Imaging Core和GEM Core紧密集成。实验病理学核心的成员在实现以服务为导向的目标以及开拓新的分子病理学研究工具方面拥有丰富的经验。核心将努力确保高质量的解释和发展工作，以促进P01的目标。