EXPERIMENTAL PATHOLOGY CORE

这一实验病理学核心是P01的一个组成部分，因为它的专业知识描述了胰腺癌从开始到转移的形态、分子和代谢表型，以及通过有针对性的基因操作和对人类肿瘤标本的研究确定的各种状态。在小鼠模型中评估的基因改变将被翻译并在临床PDAC肿瘤中进行评估以进行验证。该核心将与项目调查人员密切合作，以实现三个具体目标：1)提供组织学服务和咨询专业知识，包括集中审查小鼠和人类胰腺肿瘤的病理评估；ii)为各种 评估和实施新的体外器官培养分析以及先进的成像和分析技术，这将极大地促进未来的这些研究目标。这些目标包括在基因工程小鼠模型、小鼠异种移植模型和IRB批准的人类患者组织样本分析上执行的各种专门技术。服务将与生物库核心、成像核心和创业板核心紧密结合。实验病理学核心成员在实现以服务为导向的目标以及开拓新的分子病理学研究工具方面拥有丰富的经验。核心将努力确保高质量的解释和发展工作，以促进P01的目标。

This Experimental Pathology Core is an integral component of the P01 for its expertise in characterizing the morphologic, molecular and metabolic phenotype of pancreatic cancer, from inception to metastasis, in their diverse states determined by targeted genetic manipulations and study of human tumor specimens. Genetic alterations, as evaluated in mouse models, will be translated and assessed in clinical PDAC tumors for validation purposes. This Core will work in close collaboration with the Project Investigators to achieve three specific aims: 1) to provide histology services and consultative expertise including centralized review in the pathologic evaluation of mouse and human pancreatic neoplasms; ii) to provide infrastructure and technical expertise for a variety of molecular pathologic assays; and iii) to evaluate and implement new ex vivo organotypic culture assays and advanced imaging and analysis technologies that will greatly facilitate these research goals in future. These Aims encompass a wide variety of specialized techniques performed on genetically engineered mouse models, mouse xenograft models, and IRB-approved analysis of human patient tissue specimens. Services will be provided in close Integration with the BioBank Core, Imaging Core and GEM Core. Members of the Experimental Pathology Core have extensive experience in achieving both service-oriented goals as well as pioneering new molecular pathology research tools. The Core will seek to ensure a high quality of interpretive and developmental work to facilitate the goals of the P01.