Microscopy

显微镜检查

• 批准号: 8311795
• 负责人: TERRY A POTTER
• 金额: $ 12.54万
• 依托单位: NATIONAL JEWISH HEALTH
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-08-01 至 2014-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8311795
• 关键词: Antigens; Apoptosis; Apoptotic; B-Lymphocytes; Cells; Fluorescence Microscopy; Fluorescence Resonance Energy Transfer; Fluorochrome; Goals; Image; Intracellular Membranes; Ions; Label; Life; Lymphocyte; Measurement; Membrane Proteins; Microscopy; Movement; Protein translocation; Proteins; Research Personnel; Research Proposals; Resources; T-Lymphocyte; Tissues; cell fixing; cellular imaging; digital imaging; programs; small hairpin RNA

The goal of this core is to provide the investigators involved in this program project, state of the art digital imaging fluorescence microscopy. This includes live cell imaging of B and T cells undergoing apoptosis; rises in intracellular Ca2+, analyzing the movement of proteins tagged with GFP or other fluorochromes; measurement of FRET; analysis of protein translocations in fixed cells; determination of the efficiency of shRNA constructs and multiparameter analysis in apoptotic cells. This core will be used by projects 2 and 3 and Core C and is essential for the successful completion of the proposed Aims described therein.

这个核心的目标是为参与该计划项目的调查人员提供最先进的 数字成像荧光显微镜。这包括对B细胞和T细胞进行活细胞成像 细胞凋亡；细胞内钙离子升高，分析标记有GFP或其他标记的蛋白质的运动 荧光染料.FRET的测量.固定细胞中蛋白质易位的分析.测定 凋亡细胞中shRNA构建的效率和多参数分析。 该核心将用于项目2和3以及核心C，对于成功完成 文件中所述的拟议目标。