Tissue Culture and Virology Laboratory Core

组织培养和病毒学实验室核心

• 批准号: 8319580
• 负责人: NELL S LURAIN
• 金额: $ 20.19万
• 依托单位: RUSH UNIVERSITY MEDICAL CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8319580
• 关键词: Address; Affect; Area; Award; Bacteria; Biological Assay; Blood; Cells; Cervical; Clinical; Cloning; Cryopreserved Cell; Cytomegalovirus; Development; Disease; Ensure; Environment; Estrogens; Female; Genital system; Goals; HIV; HIV-1; Herpes Simplex Infections; Hormonal; Image; Imaging technology; Individual; Infection; Institutional Review Boards; Instruction; Intervention; Investigation; Irrigation; Knowledge; Laboratories; Link; Measures; Modeling; Molecular; Mucosal Immunity; Mucous Membrane; Pathogenesis; Patients; Performance; Peripheral Blood Mononuclear Cell; Pilot Projects; Plasma; Predisposition; Principal Investigator; Process; Production; Protocols documentation; RNA; Reagent; Research Personnel; Sampling; Signal Transduction; Specimen; Structure; Techniques; Testing; Time; Tissue Model; Tissue Sample; Tissues; Vagina; Viral Load result; Virus; Woman; Work; base; cellular imaging; design; human tissue; meetings; member; pathogen; prevent; programs; rRNA Genes; repository; research study; response; sample collection; tissue culture; transmission process; virology

The goal of the Tissue Culture and Virology Laboratory Core is to provide experimental and reagent support for the four Projects focusing on HIV-1 pathogenesis in the female genital tract and for pilot projects awarded by the Administrative Core. There are three specific aims to address this goal: 1) to perform experiments using a cervical tissue explant model; 2) to produce HIV-1-related molecular and virological reagents and perform standardized virological assays; and 3) to manage and maintain a respository containing blood and cervicovaginal lavages specimens from HIV-1 exposed or infected women. Dr. Nell Lurain will serve as the Director of the Core to oversee all activities and to communicate results to the Project Investigators. Dr. James Bremer will serve as a co-investigator to facilitate the use of the specimen repository. Drs. Lurain and Bremer have collaborated with all of the Program Investigators for over five years on other projects and will meet monthly with them to ensure that the Core program fulfills their requirements in a timely manner. In particular, the Core offers a unique expertise in the design and performance of experiments using the cervical explant model, which is highly relevant to HIV-1 disease in the female genital tract. This model will be used to measure inhibition of HIV-1 by cervical secretions from women exposed to HIV-1 who fail to become HIV-1 seropositive (Project III)or from women who have changes in the bacterial flora of the genital tract (Project II). The model will be used to study hormonal effects on HIV-1 infection (Project IV), and the model will be used to study the effects of HIV-1 co-infection with other viruses such as human cytomegalovirus and herpes simplex type-2 (Project I). The strong working relationship that has already been established between the Core Director and the Project Investigators will allow the maximum efficiency in utilizing this important model. The Core staff has molecular and virological expertise, which will provide specific reagents needed by the Projects. The knowledge and proficiency of the Core staff will provide reliable results in support of each Project, leading to a better understanding of the mechanisms of HIV-1 transmission and development of new interventions to prevent HIV-1 infection. RELEVANCE (See instructions): The purpose of Core C is to provide assays and reagents required by the four projects, which all focus on different aspects of HIV-1 infection of the female genital tract. In particular, the Core can perform experiments in cervical tissue, which is a relevant human tissue for testing conditions that affect HIV-1 replication.

组织培养和病毒学实验室核心的目标是提供实验和试剂支持 授予关注女性生殖道 HIV-1 发病机制的四个项目和试点项目 由行政核心。实现这一目标有三个具体目标：1）进行实验 使用宫颈组织外植体模型； 2) 生产HIV-1相关分子和病毒学试剂以及 进行标准化病毒学检测； 3) 管理和维护含有血液和血液的储存库 来自 HIV-1 暴露或感染妇女的宫颈阴道灌洗标本。 Nell Lurain 博士将担任 核心主任负责监督所有活动并将结果传达给项目调查员。博士。 詹姆斯·布雷默（James Bremer）将作为联合研究员，促进样本库的使用。博士。卢兰和 布雷默已与所有项目研究人员在其他项目上合作了五年多，并将 每月与他们会面，以确保核心计划及时满足他们的要求。在 特别是，核心在使用以下方法进行实验设计和执行方面提供了独特的专业知识： 宫颈外植体模型，与女性生殖道的 HIV-1 疾病高度相关。该模型将 可用于测量暴露于 HIV-1 且未能检测到的女性的宫颈分泌物对 HIV-1 的抑制作用 HIV-1 血清反应呈阳性（项目 III）或来自生殖器细菌菌群发生变化的女性 道（项目 II）。该模型将用于研究激素对 HIV-1 感染的影响（项目 IV），以及 该模型将用于研究 HIV-1 与其他病毒（例如人类病毒）同时感染的影响 巨细胞病毒和 2 型单纯疱疹病毒（项目 I）。已经建立的牢固的工作关系 核心总监和项目调查员之间建立的机制将实现最大效率 在利用这个重要模型时。核心人员拥有分子和病毒学专业知识，这将提供 项目所需的特定试剂。核心人员的知识和熟练程度将提供 支持每个项目的可靠结果，从而更好地了解 HIV-1 的机制 传播和开发新的干预措施来预防 HIV-1 感染。 相关性（参见说明）： Core C 的目的是提供四个项目所需的检测方法和试剂，这些项目都专注于 女性生殖道 HIV-1 感染的不同方面。特别是，核心可以执行 宫颈组织中的实验，宫颈组织是用于测试影响 HIV-1 的条件的相关人体组织 复制。