Tissue Culture and Virology Laboratory Core

组织培养和病毒学实验室核心的目标是提供实验和试剂支持 四个侧重于艾滋病毒-1在女性生殖道的致病机制的项目和获奖的试点项目 由管理核心执行。有三个具体的目标来解决这个目标：1)进行实验 使用宫颈组织外植体模型；2)生产与HIV-1相关的分子和病毒学试剂 执行标准化病毒学分析；以及3)管理和维护包含血液和 HIV-1暴露或感染妇女的宫颈阴道灌洗标本。内尔·卢兰博士将担任 监督所有活动，并将结果传达给项目调查员。Dr。 詹姆斯·布雷默将担任联合调查员，以促进标本储存库的使用。卢兰博士和 布雷默在其他项目上与所有项目调查人员合作了五年多，并将 每月与他们会面，确保核心计划及时满足他们的要求。在……里面 特别是，Core在设计和执行实验方面提供了独特的专业知识，使用 宫颈移植模型，这与女性生殖道的HIV-1疾病高度相关。这款车型将 用来测量暴露于HIV-1的妇女的宫颈分泌物对HIV-1的抑制作用 艾滋病毒-1血清阳性(项目III)或生殖器细菌群发生变化的妇女 区域(项目II)。该模型将用于研究荷尔蒙对HIV-1感染的影响(项目IV)，以及 模型将用于研究HIV-1与人类等其他病毒混合感染的影响 巨细胞病毒和2型单纯疱疹(项目I)。已经建立起来的牢固的工作关系 在核心主任和项目调查员之间建立合作伙伴关系将使效率达到最高 在利用这一重要模式时。核心员工拥有分子和病毒学专业知识，这将提供 项目所需的特定试剂。核心员工的知识和熟练程度将提供 支持每个项目的可靠结果，有助于更好地了解艾滋病毒-1的机制 传播和发展预防艾滋病毒-1感染的新干预措施。 相关性(请参阅说明)： 核心C的目的是提供四个项目所需的分析和试剂，这四个项目都专注于 女性生殖道感染HIV-1的不同方面。特别是，核心可以执行 在宫颈组织中进行实验，这是一种相关的人体组织，用于测试影响HIV-1的条件 复制。

The goal of the Tissue Culture and Virology Laboratory Core is to provide experimental and reagent support for the four Projects focusing on HIV-1 pathogenesis in the female genital tract and for pilot projects awarded by the Administrative Core. There are three specific aims to address this goal: 1) to perform experiments using a cervical tissue explant model; 2) to produce HIV-1-related molecular and virological reagents and perform standardized virological assays; and 3) to manage and maintain a respository containing blood and cervicovaginal lavages specimens from HIV-1 exposed or infected women. Dr. Nell Lurain will serve as the Director of the Core to oversee all activities and to communicate results to the Project Investigators. Dr. James Bremer will serve as a co-investigator to facilitate the use of the specimen repository. Drs. Lurain and Bremer have collaborated with all of the Program Investigators for over five years on other projects and will meet monthly with them to ensure that the Core program fulfills their requirements in a timely manner. In particular, the Core offers a unique expertise in the design and performance of experiments using the cervical explant model, which is highly relevant to HIV-1 disease in the female genital tract. This model will be used to measure inhibition of HIV-1 by cervical secretions from women exposed to HIV-1 who fail to become HIV-1 seropositive (Project III)or from women who have changes in the bacterial flora of the genital tract (Project II). The model will be used to study hormonal effects on HIV-1 infection (Project IV), and the model will be used to study the effects of HIV-1 co-infection with other viruses such as human cytomegalovirus and herpes simplex type-2 (Project I). The strong working relationship that has already been established between the Core Director and the Project Investigators will allow the maximum efficiency in utilizing this important model. The Core staff has molecular and virological expertise, which will provide specific reagents needed by the Projects. The knowledge and proficiency of the Core staff will provide reliable results in support of each Project, leading to a better understanding of the mechanisms of HIV-1 transmission and development of new interventions to prevent HIV-1 infection. RELEVANCE (See instructions): The purpose of Core C is to provide assays and reagents required by the four projects, which all focus on different aspects of HIV-1 infection of the female genital tract. In particular, the Core can perform experiments in cervical tissue, which is a relevant human tissue for testing conditions that affect HIV-1 replication.