Tissue Culture and Virology Laboratory Core

组织培养和病毒学实验室核心

• 批准号: 7694230
• 负责人: NELL S LURAIN
• 金额: $ 20.59万
• 依托单位: RUSH UNIVERSITY MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-30 至 2014-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7694230
• 关键词: Address; Affect; Area; Award; Bacteria; Biological Assay; Blood; Cells; Cervical; Clinical; Cloning; Cryopreserved Cell; Cytomegalovirus; Development; Disease; Ensure; Environment; Estrogens; Female; Genital system; Goals; HIV; HIV-1; Herpes Simplex Infections; Hormonal; Image; Imaging technology; Individual; Infection; Instruction; Intervention; Investigation; Irrigation; Knowledge; Laboratories; Link; Measures; Modeling; Molecular; Mucosal Immunity; Mucous Membrane; Pathogenesis; Patients; Performance; Peripheral Blood Mononuclear Cell; Pilot Projects; Plasma; Predisposition; Principal Investigator; Process; Production; Protocols documentation; RNA; Reagent; Research Ethics Committees; Research Personnel; Sampling; Signal Transduction; Specimen; Structure; Techniques; Testing; Time; Tissue Model; Tissue Sample; Tissues; Vagina; Viral Load result; Virus; Woman; Work; base; cellular imaging; design; human tissue; meetings; member; pathogen; prevent; programs; rRNA Genes; repository; research study; response; sample collection; tissue culture; transmission process; virology

The goal of the Tissue Culture and Virology Laboratory Core is to provide experimental and reagent support for the four Projects focusing on HIV-1 pathogenesis in the female genital tract and for pilot projects awarded by the Administrative Core. There are three specific aims to address this goal: 1) to perform experiments using a cervical tissue explant model; 2) to produce HIV-1-related molecular and virological reagents and perform standardized virological assays; and 3) to manage and maintain a respository containing blood and cervicovaginal lavages specimens from HIV-1 exposed or infected women. Dr. Nell Lurain will serve as the Director of the Core to oversee all activities and to communicate results to the Project Investigators. Dr. James Bremer will serve as a co-investigator to facilitate the use of the specimen repository. Drs. Lurain and Bremer have collaborated with all of the Program Investigators for over five years on other projects and will meet monthly with them to ensure that the Core program fulfills their requirements in a timely manner. In particular, the Core offers a unique expertise in the design and performance of experiments using the cervical explant model, which is highly relevant to HIV-1 disease in the female genital tract. This model will be used to measure inhibition of HIV-1 by cervical secretions from women exposed to HIV-1 who fail to become HIV-1 seropositive (Project III)or from women who have changes in the bacterial flora of the genital tract (Project II). The model will be used to study hormonal effects on HIV-1 infection (Project IV), and the model will be used to study the effects of HIV-1 co-infection with other viruses such as human cytomegalovirus and herpes simplex type-2 (Project I). The strong working relationship that has already been established between the Core Director and the Project Investigators will allow the maximum efficiency in utilizing this important model. The Core staff has molecular and virological expertise, which will provide specific reagents needed by the Projects. The knowledge and proficiency of the Core staff will provide reliable results in support of each Project, leading to a better understanding of the mechanisms of HIV-1 transmission and development of new interventions to prevent HIV-1 infection. RELEVANCE (See instructions): The purpose of Core C is to provide assays and reagents required by the four projects, which all focus on different aspects of HIV-1 infection of the female genital tract. In particular, the Core can perform experiments in cervical tissue, which is a relevant human tissue for testing conditions that affect HIV-1 replication.

组织培养和病毒学实验室核心的目标是提供实验和试剂支持 四个重点关注女性生殖道中HIV-1发病机制的项目和试点项目 行政核心。有三个具体目标来解决这个目标：1）进行实验 使用宫颈组织外植体模型; 2）生产HIV-1相关的分子和病毒学试剂， 进行标准化的病毒学检测; 3）管理和维护血液储存库， 来自HIV-1暴露或感染妇女的宫颈阴道灌洗标本。Nell Lurain博士将担任 核心总监负责监督所有活动，并将结果传达给项目研究者。博士 James Bremer将担任共同研究员，以促进标本库的使用。卢雷恩医生和 布雷默与所有的计划调查人员合作了五年多的其他项目，并将 每月与他们会面，以确保核心计划及时满足他们的要求。在 特别是，核心提供了一个独特的专业知识，在设计和性能的实验，使用 宫颈移植模型，这是高度相关的HIV-1疾病的女性生殖道。这种模式的 用于测量暴露于HIV-1但未能 HIV-1血清反应呈阳性（项目III），或来自生殖器细菌植物群发生变化的妇女 （项目二）。该模型将用于研究激素对HIV-1感染的影响（项目IV）， 该模型将用于研究HIV-1与其他病毒（如人类）共感染的影响 巨细胞病毒和单纯疱疹2型（项目I）。强大的工作关系已经 在核心主任和项目调查员之间建立一个协调机制， 利用这一重要模式。核心工作人员拥有分子和病毒学专业知识， 项目所需的特定试剂。核心工作人员的知识和熟练程度将提供 支持每个项目的可靠结果，从而更好地了解HIV-1的机制 传播和发展新的干预措施，以防止艾滋病毒1感染。 相关性（参见说明）： 核心C的目的是提供四个项目所需的测定和试剂，这些项目都侧重于 女性生殖道HIV-1感染的不同方面。特别是，核心可以执行 在宫颈组织中进行的实验，宫颈组织是用于测试影响HIV-1的条件的相关人体组织 复制的