Immune responses to Norovirus after natural infection in Vietnamese children and

描述(由申请人提供)：此FIRCA-BB申请旨在通过与俄亥俄州辛辛那提辛辛那提儿童医院医学中心(CCHMC，酱，Pi)的著名NV研究实验室合作，发展越南国家卫生与流行病学研究所(NIHE)的LMIC实验室(Nguyen Van Trang，co-Pi)进行诺沃克病毒(NV)研究的能力。我们将对越南儿童NV相关性胃肠炎进行现场监测，重点关注病毒/宿主相互作用、免疫学和NV进化这三个当前急需解决的问题。我们将确定组织血型抗原(HBGA)状态与急性胃肠炎住院儿童对新城疫的易感性的相关性；在临床和社区环境中评估儿童自然感染新城疫后的免疫反应；并通过越南儿童GII.4变异的基因和抗原特征，研究宿主群体免疫在新城疫进化中的作用。为了提高LMIC实验室的研究能力，我们将特别注重完善监测网络、培训实验室人员以及转让或改造新技术，包括根据LMIC实验室的需要开发新技术。我们的目标是使LMIC实验室成为国家参考实验室，使Trang博士成为越南NV和轮状病毒研究的全国领先者。 公共卫生相关性：FIRCA-BB应用程序将对越南儿童中诺如病毒(NV)相关性胃肠炎进行现场监测，重点关注目前NV研究中的三个紧迫主题。我们将确定组织血型抗原(HBGA)状态与急性胃肠炎住院儿童对新城疫的易感性的相关性；在临床和社区环境中评估儿童自然感染新城疫后的免疫反应；并通过越南儿童GII.4变异的基因和抗原特征，研究宿主群体免疫在新城疫进化中的作用。作为进一步的目标，我们还将重点发展我们的合作者在越南的实验室(阮文庄，共同派)在现场监测和实验室诊断新城疫和相关疾病方面的能力。我们的目标是使该实验室成为国家参考实验室，并使Trang博士成为越南NV和轮状病毒研究的全国领先者。

DESCRIPTION (provided by applicant): This FIRCA-BB application intends to having a capacity development of a LMIC laboratory (Nguyen Van Trang, co-PI) at the National Institute of Hygiene and Epidemiology (NIHE) in Vietnam on norovirus (NV) research through collaboration with a well established NV research laboratory at the Cincinnati Children's Hospital Medical Center (CCHMC, Jiang, PI) in Cincinnati, Ohio. We will perform field surveillance of NV associated gastroenteritis in Vietnamese children focusing on three currently urgent topics on the virus/host interaction, immunology and evolution of NVs. We will determine the correlation of histo-blood group antigen (HBGA) status with susceptibility to NVs in children hospitalized with acute gastroenteritis; access immune responses to NVs following natural infection of children in clinical and community settings; and study the role of host herd immunity in NV evolution by genetic and antigenic characterization of GII.4 variants in the Vietnamese children. To improve the research capacity of the LMIC laboratory, we will particularly focus on the improvement of the surveillance network, training of laboratory personnel, and transferring or adapting of new technology, including developing new ones based on needs of the LMIC laboratory. Our goal is to have the LMIC laboratory becoming a national reference laboratory and Dr. Trang the national leader in the NV and rotavirus researches in Vietnam. PUBLIC HEALTH RELEVANCE: This FIRCA-BB application will perform field surveillance of noroviruses (NV) associated gastroenteritis in the Vietnamese children focusing on three currently urgent topics on NV research. We will determine the correlation of histo-blood group antigen (HBGA) status with susceptibility to NVs in children hospitalized with acute gastroenteritis; access immune responses to NVs following natural infection of children in clinical and community settings; and study the role of host herd immunity in NV evolution by genetic and antigenic characterization of GII.4 variants in the Vietnamese children. As a further goal we also will focus on the capacity development of our collaborator's laboratory (Nguyen Van Trang, co-PI) in Vietnam on the field surveillance and laboratory diagnosis of NVsand associated illness. Our goal is to have the laboratory become a national reference laboratory and Dr. Trang the national leader in the NV and rotavirus researches in Vietnam.