Biomolecule Production

作为新英格兰地区生物防御和新发传染病卓越中心的一部分 (NERCE/BEID)，生物分子生产核心实验室为生产介质到 向调查人员提供大量纯化的细菌、哺乳动物和病毒生物分子 生物防御研究。这个核心设施是一个最先进的实验室，旨在生产两种重组 用于结构分析和疫苗配方的蛋白质和天然碳水化合物分子。这 包括但不限于下列类别的调查人员：利用 蛋白质组学和小分子筛选需要生产足够数量的蛋白质的核心实验室 确定蛋白质以完成拟议的筛选；那些开发含有细菌的疫苗的蛋白质 需要大量抗原进行体外生物检测和分析的碳水化合物成分 动物研究；以及那些已经使用重组系统小规模表达目标蛋白的人 需要更多的重组蛋白来扩大他们的研究。调查人员提供数据 展示生产和纯化方法的有效性以及菌株或重组质粒 如适用，请做好准备工作。实验室扩大了这些方案的生产和提纯 将目标生物分子的镁的100‘S连同概述方法的报告一起交付给研究人员 使用。自2004年生物分子生产核心成立以来，我们已经与19名研究人员合作 来自8家研究机构和1家公司生产了154多种不同的生物分子。我们有 进行了339次发酵生产生物分子，产生了52公斤的细菌细胞。 已经完成了277次提纯，我们已经向调查人员交付了34公斤的细胞浆， 纯化碳水化合物453 mg，各种纯化重组蛋白3.1g。

As part of the New England Regional Center of Excellence for Biodefense and Emerging Infectious Diseases (NERCE/BEID), the Biomolecule Production Core Laboratory provides services for production of medium to large scale amounts of purified bacterial, mammalian, and viral biomolecules to investigators conducting biodefense research. This core facility is a state-of-the-art laboratory designed to produce both recombinant proteins and native carbohydrate molecules for use in structural analyses and vaccine formulation. This includes, but is not restricted to, investigators in the following categories: those making use of the Proteomics and Small Molecule Screening Core Laboratories who need to produce sufficient quantities of identified proteins to accomplish proposed screens; those developing vaccines containing bacterial carbohydrate components who need large quantities of antigen for analysis in vitro biologic assays and animal studies; and those already expressing target proteins in small scale using recombinant systems who need larger amounts of recombinant protein to expand their research. Investigators provide data demonstrating efficacy of production and purification methods as well as strains or recombinant plasmid preparations where applicable. The laboratory scales-up these protocols for production and purification of 100's of mg of the target biomolecule that is delivered to the investigator with a report outlining the methods used. Since the Biomolecule Production Core opened in 2004, we have collaborated with 19 investigators from 8 research institutions and one company in the production of over 154 different biomolecules. We have conducted 339 fermentation runs for the production of biomolecules, generating 52 Kg of bacterial cells. More than 277 purifications have been completed and we have delivered to investigators 34 Kg of cell paste, 453 mg of purified carbohydrate, and 3.1 g of various purified recombinant protein.