Nedd4-family adaptors and their regulation of T cell function.

Nedd4 家族接头及其对 T 细胞功能的调节。

• 批准号: 8220754
• 负责人: Paula Maria Oliver
• 金额: $ 41.88万
• 依托单位: CHILDREN'S HOSP OF PHILADELPHIA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-02-04 至 2016-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8220754
• 关键词: Accounting; Affinity; Antigens; Asthma; Atopic Dermatitis; Autoantigens; Autoimmune Diseases; CD28 gene; CD3 Antigens; Cell physiology; Cells; Data; Defect; Disease; Failure; Family; Food; Food Hypersensitivity; Gastrointestinal tract structure; Genetic Polymorphism; Goals; Human; IL2RA gene; IgE; Immune system; In Vitro; Inflammation; Inflammatory; Inflammatory Bowel Diseases; Inflammatory Response; Interleukin-2; Interleukin-4; Laboratories; Ligands; Lung; Mediating; Mucosal Immunity; Mucous Membrane; Mus; Oral; Ovum; Pathway interactions; Patients; Peptides; Phenotype; Polymorphism Analysis; Production; Proliferating; Proteins; Rag1 Mouse; Receptor Signaling; Regulation; Signal Transduction; Single Nucleotide Polymorphism; Site; Skin; Surface; T-Cell Activation; T-Lymphocyte; Testing; Th2 Cells; Tissues; Work; commensal microbes; cytokine; design; feeding; in vivo; man; prevent; public health relevance; receptor; response; transcription factor; ubiquitin-protein ligase

DESCRIPTION (provided by applicant): Mucosal surfaces are continuously exposed to harmless foreign antigens such as those from food and commensal bacteria, referred to as environmental antigens. The immune system should be tolerant to these environmental antigens, much as it is to self-antigens. While some mechanisms that regulate tolerance to self and environmental antigens may be shared, others may be unique. We have identified an E3 ubiquitin ligase adaptor, known as Nedd4-family interacting protein 1 (Ndfip1), that regulates T cell tolerance to environmental antigens in mice and man. We recently showed that mice lacking Ndfip1 develop atopic inflammation in lung, skin and GI tract. Additionally, using single nucleotide polymorphism (SNP) analysis, we have identified polymorphisms within the locus that encodes Ndfip1 (located on human Chr5) that are more common in patients with asthma, atopic dermatitis (AD) and inflammatory bowel disease (IBD)2, thus supporting that Ndfip1 regulates atopic disease in both mice and man. Studying Ndfip1-/- mice, we have determined that T cells lacking Ndfip1 become activated and produce Th2 cytokines in response to environmental antigens. We hypothesize that Ndfip1 regulates T cell tolerance by promoting the differentiation of na¿ve T cells into iTregs and by inducing antigen-unresponsiveness. In this proposal, we will 1) determine the mechanism by which Ndfip1 promotes iTreg differentiation by determining whether Ndfip1 is required for iTreg conversion in vitro and in vivo and resolving whether the defect in iTreg conversion of Ndfip1-/- T cells is cell intrinsic or due to their production of IL-4. We will also establish whether Ndfip1 is required for Itch ubiquitylation of TIEG1. We will also 2) test whether Ndfip1-/- T cells require CD28-costimulation to become activated in vivo, use altered peptide ligands in vivo and in vitro to establish whether Ndfip1-/- T cells are activated by lower affinity antigens than WT cells, and determine whether Ndfip1 promotes antigen unresponsiveness in wild type na¿ve T cells by dampening TCR or IL-2R signaling. Finally, we will establish the mechanism(s) underlying these defects. We believe that Ndfip1-dependent pathways could be targeted therapeutically to treat atopic inflammatory diseases such as asthma as well as T cell mediated autoimmune disease. A long-term goal of the laboratory is to design pharmacological mimics of Ndfip1 that would promote its functions therapeutically. The studies we propose will help us toward this goal. PUBLIC HEALTH RELEVANCE: In this proposal, we will determine whether and how Ndfip1 regulates T cell tolerance to environmental antigens. Understanding how this works on a mechanistic level could have broad impact to food allergy and mucosal immunity and to atopic diseases in general. Thus, we believe that Ndfip1-dependent pathways could be targeted therapeutically to treat atopic inflammatory diseases such as asthma, food allergy and atopic dermatitis.

描述（由申请人提供）：粘膜表面持续暴露于无害的外来抗原，例如来自食物和共生细菌的抗原，称为环境抗原。免疫系统应该能够耐受这些环境抗原，就像耐受自身抗原一样。虽然调节对自身和环境抗原的耐受性的一些机制可能是共享的，但其他机制可能是独特的。我们已经鉴定出一种 E3 泛素连接酶接头，称为 Nedd4 家族相互作用蛋白 1 (Ndfip1)，它可以调节小鼠和人类 T 细胞对环境抗原的耐受性。我们最近发现，缺乏 Ndfip1 的小鼠会在肺部、皮肤和胃肠道中出现特应性炎症。此外，利用单核苷酸多态性 (SNP) 分析，我们发现了编码 Ndfip1（位于人类 Chr5 上）的基因座内的多态性，这些多态性在哮喘、特应性皮炎 (AD) 和炎症性肠病 (IBD) 患者中更为常见2，从而支持 Ndfip1 调节小鼠和人类的特应性疾病。通过研究 Ndfip1-/- 小鼠，我们确定缺乏 Ndfip1 的 T 细胞会被激活并产生 Th2 细胞因子以响应环境抗原。我们假设 Ndfip1 通过促进幼稚 T 细胞分化为 iTreg 并诱导抗原无反应来调节 T 细胞耐受。在本提案中，我们将1）通过确定Ndfip1在体外和体内的iTreg转化是否需要Ndfip1来确定Ndfip1促进iTreg分化的机制，并解决Ndfip1-/- T细胞的iTreg转化缺陷是细胞内在的还是由于其产生IL-4所致。我们还将确定 Ndfip1 是否是 TIEG1 的 Itch 泛素化所必需的。我们还将 2) 测试 Ndfip1-/- T 细胞是否需要 CD28 共刺激才能在体内激活，在体内和体外使用改变的肽配体来确定 Ndfip1-/- T 细胞是否被比 WT 细胞亲和力更低的抗原激活，并确定 Ndfip1 是否通过抑制 TCR 或 IL-2R 来促进野生型幼稚 T 细胞的抗原无反应 发信号。最后，我们将建立这些缺陷背后的机制。我们相信 Ndfip1 依赖性途径可以作为治疗靶点来治疗特应性炎症性疾病，例如哮喘以及 T 细胞介导的自身免疫性疾病。该实验室的长期目标是设计 Ndfip1 的药理学模拟物，以促进其治疗功能。我们提出的研究将帮助我们实现这一目标。 公共健康相关性：在本提案中，我们将确定 Ndfip1 是否以及如何调节 T 细胞对环境抗原的耐受性。了解其在机制层面上的工作原理可能会对食物过敏和粘膜免疫以及一般的特应性疾病产生广泛的影响。因此，我们相信 Ndfip1 依赖性途径可以作为治疗靶点来治疗特应性炎症性疾病，例如哮喘、食物过敏和特应性皮炎。