Cell Culture Core

细胞培养核心实验室的主要目标是维持充足的培养细胞供应 对于本计划项目申请的个别项目中提出的各种研究，以及 为这项服务提供最高水平的质量控制。此外，核心将继续作为一个 培训项目实验室成员正确的细胞培养方法和方法的教育设施 质量控制。 在前一次资助期间，该核心实验室达到了其目标，提供了 拥有足够数量的特化培养细胞进行研究的研究人员。绝大多数的美国人 该计划项目发布的报告包含依赖于正确操作的实验 这个核心实验室的。来自项目实验室的数十人接受了细胞培养方面的培训 方法和设备已用于在实验室中由个人进行的细胞培养研究 当他们自己的实验室设施被占用时，项目负责人。尽管所有项目负责人都在维护“卫星” 细胞培养设施，这些研究人员一直完全依赖于核心实验室的库存烧瓶 细胞。核心实验室人员已经建立了无数长期培养的牛主动脉内皮细胞 细胞、人脐静脉内皮细胞、小鼠主动脉内皮细胞和人包皮成纤维细胞。许多已建立的细胞 还定期提供线路。 该设施的质量控制一直很好，几乎没有发生微生物污染事件 尽管有许多人共享这个设施，但已经有好几年了。这一成功反映了这样一个事实，即使用 都需要经过最严格的哺乳动物细胞培养培训。这次培训将 继续提供给所有进行细胞培养实验的计划项目科学家。

The principal objective of the Cell Culture Core Laboratory is to maintain a sufficient supply of cultured cells for the various studies that are proposed in the individual projects of this Program Project application and to provide this service with the highest level of quality control. In addition, the Core will continue to function as an educational facility in training members of the Program Project laboratories in proper cell culture methods and quality control. During the previous funding period this core laboratory has met its objective with respect to providing program investigators with sufficient numbers of characterized cultured cells for their studies. The great majority of the published reports from the program project contain experiments that were dependent on the proper operation of this core laboratory. Dozens of individuals from the project laboratories have been trained in cell culture methods and the facility has been used for cell culture studies performed by individuals in the laboratories of Project Leaders when their own lab facilities were occupied. Although all Project Leaders maintain "satellite" cell culture facilities, these investigators have been fully dependent on the core laboratory for stock flasks of cells. Core laboratory personnel have established countless long-term cultures of bovine aortic endothelial cells (EC), human umbilical vein EC, mouse aortic EC, and human foreskin fibroblasts. Many established cell lines have also been provided on a regular basis. Quality control in the facility has been excellent with minimal incidents of microbiologic contamination over the years in spite of the many individuals sharing the facility. This success reflects the fact that those using the facility are required to go through the most rigorous of mammalian cell culture training. This training will continue to be provided to all Program Project scientists performing cell culture experiments.