Cell Culture Core

细胞培养核心实验室的主要目标是维持足够的培养细胞供应 对于在本计划项目申请的各个项目中提出的各种研究， 以最高水平的质量控制提供此服务。此外，核心将继续发挥作用， 教育设施，培训计划项目实验室成员正确的细胞培养方法， 质量控制 在上一个资助期内，该核心实验室实现了提供项目的目标。 研究人员有足够数量的特征培养细胞用于他们的研究。绝大多数 该计划项目的公开报告包含了依赖于正确操作的实验 这个核心实验室。来自项目实验室的数十人接受了细胞培养培训 方法和设施已被用于细胞培养研究进行的个人在实验室的 当他们自己的实验室设施被占用时，项目负责人。尽管所有项目负责人都保持“卫星” 细胞培养设施，这些研究人员一直完全依赖于核心实验室的储备烧瓶， 细胞核心实验室人员已经建立了无数的牛主动脉内皮细胞的长期培养物， 细胞（EC）、人脐静脉EC、小鼠主动脉EC和人包皮成纤维细胞。许多已建立的细胞 还定期提供热线电话。 该设施的质量控制非常好， 多年来，尽管许多人分享设施。这一成功反映了这样一个事实，即那些使用 实验室的所有人都必须经过最严格的哺乳动物细胞培养培训。本次培训将 继续提供给所有进行细胞培养实验的计划项目科学家。

The principal objective of the Cell Culture Core Laboratory is to maintain a sufficient supply of cultured cells for the various studies that are proposed in the individual projects of this Program Project application and to provide this service with the highest level of quality control. In addition, the Core will continue to function as an educational facility in training members of the Program Project laboratories in proper cell culture methods and quality control. During the previous funding period this core laboratory has met its objective with respect to providing program investigators with sufficient numbers of characterized cultured cells for their studies. The great majority of the published reports from the program project contain experiments that were dependent on the proper operation of this core laboratory. Dozens of individuals from the project laboratories have been trained in cell culture methods and the facility has been used for cell culture studies performed by individuals in the laboratories of Project Leaders when their own lab facilities were occupied. Although all Project Leaders maintain "satellite" cell culture facilities, these investigators have been fully dependent on the core laboratory for stock flasks of cells. Core laboratory personnel have established countless long-term cultures of bovine aortic endothelial cells (EC), human umbilical vein EC, mouse aortic EC, and human foreskin fibroblasts. Many established cell lines have also been provided on a regular basis. Quality control in the facility has been excellent with minimal incidents of microbiologic contamination over the years in spite of the many individuals sharing the facility. This success reflects the fact that those using the facility are required to go through the most rigorous of mammalian cell culture training. This training will continue to be provided to all Program Project scientists performing cell culture experiments.