REDOX BIOMARKERS

氧化还原生物标志物

• 批准号: 8250452
• 负责人: Peter Libby
• 金额: $ 10.71万
• 依托单位: BRIGHAM AND WOMEN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-01 至 2015-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8250452
• 关键词: Adipose tissue; Animal Model; Aortic Aneurysm; Area; Arterial Fatty Streak; Atherosclerosis; Biochemical; Biological Assay; Biological Markers; Biological Preservation; Blood Vessels; Blood specimen; Clinical; Collection; Consultations; Cryoultramicrotomy; Data; Disease; Endothelial Cells; Ensure; Equipment; Eugenia; Experimental Designs; Fatty Acids; Foundations; Freezing; Functional disorder; Human; Human Resources; Hypochlorous Acid; Immunofluorescence Immunologic; Immunofluorescence Microscopy; Individual; Inflammation; Life; Lipoproteins; Low-Density Lipoproteins; Manuals; Measurement; Measures; Microscopic; Modeling; Mus; Nonesterified Fatty Acids; Obesity; Oxidation-Reduction; Oxidative Stress; Oxidative Stress Pathway; Peroxidases; Plasma; Preparation; Process; Proteins; Publications; Publishing; Quality Control; Research Personnel; Resources; Sampling; Serum; Services; Specimen; Stress; Supervision; Techniques; Testing; Time; Tissue Banking; Tissue Banks; Tissue Sample; Tissues; Vascular Diseases; Weight; Work; cytokine; design; experience; human tissue; in vivo; macrophage; molecular imaging; novel; operation; programs; research study; vascular inflammation

Core D will provide support for histochemical and immunofluorescence microscopy of vascular and adipose tissues prepared from the various mouse preparations being generated in this Program. Morphological analyses will focus on analyzing tissue markers of vascular inflammation and redox stress using histochemical and immunofluorescence approaches, as well as serum, measurements of cytokines, lipoproteins, and fatty acids. Specific experiments planned with support from Core D are presented in the individual project descriptions. Core D also will provide centralized collection, processing, and quantitative analysis of biomarkers of oxidative stress and inflammation of tissue specimens for the individual projects. This facility will primarily use histopathological, microscopic, and biochemical approaches. The quantification and characterization of this vascular disease will build on our extensive experience in analysis of both human tissue specimens, particularly atheromata, and Murine models of atherosclerosis, aortic aneurysms, and obesity. During its 15 year life, this core of our program project has built a tissue bank of more than 800 human atherosclerotic specimens preserved for a variety of analytic techniques, and quality controlled. Retrospective quality control of specimens was routinely performed by Eugenia Shvartz under supervision of Dr. Sukhova, according to a formal manual of operations. Two to four samples per year (1994 - 1999) will be used for quality immunostaining for macrophages, SMC, and endothelial cells. Recent test revealed perfect preservation and quality of cryosections obtained from 2000 -2006. Protein extracted from tissue samples snap frozen in 2000, 2001, 2004, 2005, and 2007 showed similar yield, 5.5 -11pg/mg of dry weight independently of storage time (- 80¿C). This core will also measure several plasma components integral to the experimental design of the majority of projects. Over its life, this core has supported more than one hundred of publications.

核心D将为本计划中生成的各种小鼠制备物制备的血管和脂肪组织的组织化学和免疫荧光显微镜检查提供支持。形态学分析将侧重于使用组织化学和免疫荧光方法分析血管炎症和氧化还原应激的组织标志物，以及血清、细胞因子、脂蛋白和脂肪酸的测量。在核心D的支持下计划进行的具体实验见各个项目说明。 核心D还将为各个项目提供组织标本的氧化应激和炎症生物标志物的集中收集、处理和定量分析。该机构将主要使用组织病理学、显微镜和生物化学方法。这种血管疾病的定量和表征将建立在我们在分析人体组织标本（特别是动脉粥样硬化）和动脉粥样硬化、主动脉瘤和肥胖的小鼠模型方面的丰富经验基础上。在其15年的生命周期中，我们项目的核心已经建立了一个超过800个人类动脉粥样硬化标本的组织库，这些标本被保存用于各种分析技术和质量控制。根据一份报告，在Sukhova博士的监督下，Eugenia Shvartz定期对标本进行回顾性质量控制。 正式的操作手册。每年（1994 - 1999年）将使用两至四个样本进行质量评估 巨噬细胞、SMC和内皮细胞的免疫染色。最近的测试显示，2000 - 2006年获得的冷冻切片保存完好，质量良好。从2000年、2001年、2004年、2005年和2007年速冻的组织样品中提取的蛋白质显示出相似的产量，5.5 - 11 pg/mg干重，与储存时间（-80 ℃）无关。该核心还将测量几个等离子体成分，这些成分是大多数项目实验设计的组成部分。在它的生命中，这个核心已经支持了一百多个出版物。