Mechanisms and Modulation of Accelerated Atherosclerosis in Clonal Hematopoiesis
克隆造血加速动脉粥样硬化的机制和调节
基本信息
- 批准号:10590675
- 负责人:
- 金额:$ 65.04万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2022
- 资助国家:美国
- 起止时间:2022-04-01 至 2026-02-28
- 项目状态:未结题
- 来源:
- 关键词:AblationAccelerationAgeAgingAntibodiesArterial Fatty StreakAtherosclerosisBiologyBloodBone MarrowBone Marrow CellsBone Marrow Stem CellCardiovascular systemCellsChimera organismChimeric ProteinsClinicalClinical DataDNMT3aDataEndotheliumEtiologyEventEvolutionFemaleGenotypeGonadal Steroid HormonesHematopoiesisHost DefenseHumanIL-6 inhibitorIL6ST geneIgG1In SituIndividualInfectionInflammasomeInflammationInflammatoryInterleukin 6 ReceptorInterleukin-1Interleukin-1 betaInterleukin-6InterleukinsInterruptionLesionLeukocytesLoxP-flanked alleleMacrophageMediatorMessenger RNAMonitorMononuclearMusMutationMyelogenousMyeloid CellsOncologyOrganOutcomePTPRC genePathway interactionsPhagocytesPopulationProceduresProteinsRecurrenceResearchRiskRisk FactorsRoleScienceSex DifferencesSignal TransductionSmooth Muscle MyocytesSomatic MutationSpleenTestingWorkatherogenesisatherothrombosiscardiovascular risk factorclinical investigationcytokineexperienceexperimental studygenetic variantimprovedinfection riskinterleukin-6 receptor alphamalemutantnovelperipheral bloodpreservationresponsesingle-cell RNA sequencingtargeted treatmenttranslational potential
项目摘要
Project Summary/Abstract
With age, humans can accumulate leukocyte clones in blood that arise from somatic mutations in bone marrow
stem cells that enhance expansion: clonal hematopoiesis of indeterminate potential (CHIP). Mutations in
DNMT3A and TET2 account for the plurality of these clones. CHIP confers highly elevated cardiovascular (CV)
risk, independent of traditional risk factors. We have found accelerated atherogenesis and the involvement of
IL-1 and IL-6 in mice with myeloid loss of Tet2 or Dnmt3a function and that genetically reduced IL-6 signaling
abrogates the elevated CV risk in humans with DNMT3A or TET2 CHIP. Analyses of our CANTOS trial showed
greater efficacy of IL-1β inhibition in humans with DNMT3A or TET2 CHIP. These results point the way to a
genotype-directed allocation of therapy, an approach that has transformed oncology but remains aspirational in
atherosclerosis. The roles of classical vs. trans IL-6 signaling in atherothrombosis requires further study due to
conflicting evidence. Specific aim 1 will test the hypothesis that atherosclerotic mice that mimic CHIP due to
myeloid deficiency in Dnmt3a treated with an antibody that interrupts global IL-6 signaling by neutralizing IL-6
receptor α (IL-6r, CD126) have decreased atherosclerosis and inflammation within the lesions, blood and other
organs as gauged in part by single-cell RNA sequencing (scRNA-seq). We further hypothesize that IL-6r
inhibition will limit expansion of the mutant clone. Specific aim 2 will probe the role of classical vs. trans IL-6
signaling in myeloid cells in accelerated atherogenesis in CHIP using Il6rflox/flox/Lyz2-Cre mice bone marrow
chimeric LDLR-/- mice (to block leukocyte classical signaling), and administration of a gp130-IgG1-Fc chimeric
protein (to block trans signaling) using similar procedures and endpoints. The results will illuminate the
controversy and unsettled science regarding the contributions of classical and trans IL-6 signaling to
atherogenesis. Specific aim 3. Our preliminary experiments show that female myeloidTet2-/-Ldlr-/- mice have
greater acceleration of atherogenesis than Tet2+/+ Ldlr-/- male mice and, unlike their male counterparts, show
reduced atherogenesis with IL-1β neutralization. We will localize where this sex difference operates in the
inflammasome–IL-1β–IL-6 pathway, and test the hypothesis that female Dnmt3a-/-Ldlr-/- mice have greater
response to IL-6r inhibition than males. We will probe mechanisms by gonadal ablation experiments and by
analysis of scRNA-seq data, which in preliminary data shows IL-1β expression in resident macrophages cells
from atheroma from Tet2-/- females but not males. This work will deepen understanding of the mechanisms of
accelerated atherosclerosis in CHIP. Our new pilot clinical data show that an anti-IL-6 antibody can mute
inflammation in humans. Thus, the work proposed here will provide an indispensable step toward validating
and furnishing the fundamental basis of an immediately translatable targeted therapeutic strategy based on
CHIP genotype.
项目总结/文摘
项目成果
期刊论文数量(0)
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科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Peter Libby其他文献
Peter Libby的其他文献
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{{ truncateString('Peter Libby', 18)}}的其他基金
Role of eosinophil cationic proteins in cardiac hypertrophy
嗜酸性粒细胞阳离子蛋白在心脏肥大中的作用
- 批准号:
10735136 - 财政年份:2023
- 资助金额:
$ 65.04万 - 项目类别:
Mechanisms and Modulation of Accelerated Atherosclerosis in Clonal Hematopoiesis
克隆造血加速动脉粥样硬化的机制和调节
- 批准号:
10418315 - 财政年份:2022
- 资助金额:
$ 65.04万 - 项目类别:
Novel mechanisms of the thrombotic complications of atherosclerosis
动脉粥样硬化血栓并发症的新机制
- 批准号:
9977702 - 财政年份:2019
- 资助金额:
$ 65.04万 - 项目类别:
Novel mechanisms of the thrombotic complications of atherosclerosis
动脉粥样硬化血栓并发症的新机制
- 批准号:
10191002 - 财政年份:2019
- 资助金额:
$ 65.04万 - 项目类别:
Novel mechanisms of the thrombotic complications of atherosclerosis
动脉粥样硬化血栓并发症的新机制
- 批准号:
10428552 - 财政年份:2019
- 资助金额:
$ 65.04万 - 项目类别:
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