REDOX BIOMARKERS

氧化还原生物标志物

• 批准号: 7975792
• 负责人: Peter Libby
• 金额: $ 10.69万
• 依托单位: BRIGHAM AND WOMEN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-04-21 至 2015-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7975792
• 关键词: Adipose tissue; Animal Model; Aortic Aneurysm; Area; Arterial Fatty Streak; Atherosclerosis; Biochemical; Biological Assay; Biological Markers; Biological Preservation; Blood Vessels; Blood specimen; Clinical; Collection; Consultations; Core Facility; Cryoultramicrotomy; Data; Disease; Endothelial Cells; Ensure; Equipment; Eugenia; Experimental Designs; Fatty Acids; Foundations; Freezing; Human; Human Resources; Hypochlorous Acid; Immunofluorescence Immunologic; Immunofluorescence Microscopy; Individual; Inflammation; Life; Lipoproteins; Low-Density Lipoproteins; Manuals; Measurement; Measures; Microscopic; Modeling; Mus; Nonesterified Fatty Acids; Obesity; Oxidation-Reduction; Oxidative Stress; Oxidative Stress Pathway; Peroxidases; Plasma; Preparation; Process; Proteins; Publications; Publishing; Quality Control; Research Personnel; Resources; Sampling; Serum; Services; Specimen; Stress; Supervision; Techniques; Testing; Time; Tissue Banking; Tissue Banks; Tissue Sample; Tissues; Vascular Diseases; Weight; Work; cytokine; design; experience; human tissue; in vivo; macrophage; molecular imaging; novel; operation; programs; research study; vascular inflammation

Core D will provide support for histochemical and immunofluorescence microscopy of vascular and adipose tissues prepared from the various mouse preparations being generated in this Program. Morphological analyses will focus on analyzing tissue markers of vascular inflammation and redox stress using histochemical and immunofluorescence approaches, as well as serum, measurements of cytokines, lipoproteins, and fatty acids. Specific experiments planned with support from Core D are presented in the individual project descriptions. Core D also will provide centralized collection, processing, and quantitative analysis of biomarkers of oxidative stress and inflammation of tissue specimens for the individual projects. This facility will primarily use histopathological, microscopic, and biochemical approaches. The quantification and characterization of this vascular disease will build on our extensive experience in analysis of both human tissue specimens, particularly atheromata, and Murine models of atherosclerosis, aortic aneurysms, and obesity. During its 15 year life, this core of our program project has built a tissue bank of more than 800 human atherosclerotic specimens preserved for a variety of analytic techniques, and quality controlled. Retrospective quality control of specimens was routinely performed by Eugenia Shvartz under supervision of Dr. Sukhova, according to a formal manual of operations. Two to four samples per year (1994 - 1999) will be used for quality immunostaining for macrophages, SMC, and endothelial cells. Recent test revealed perfect preservation and quality of cryosections obtained from 2000 -2006. Protein extracted from tissue samples snap frozen in 2000, 2001, 2004, 2005, and 2007 showed similar yield, 5.5 -11pg/mg of dry weight independently of storage time (- 80¿C). This core will also measure several plasma components integral to the experimental design of the majority of projects. Over its life, this core has supported more than one hundred of publications.

核心D将提供支持该程序中生成的各种小鼠制剂制备的血管和脂肪时序的组织化学和免疫荧光显微镜的支持。形态学分析将使用组织化学和免疫荧光方法以及血清，细胞因子，脂蛋白和脂肪酸的测量，将重点放在分析的血管注射和氧化还原胁迫的组织标记上。在单个项目描述中介绍了在核心D支持下计划的具体实验。 核心D还将对单个项目的氧化应激和组织样本注射的生物标志物进行集中收集，加工和定量分析。该设施将主要使用组织病理学，微观和生化方法。这种血管疾病的定量和表征将基于我们在分析人类组织标本的广泛经验，尤其是动脉粥样硬化，动脉粥样硬化，主动脉动脉瘤和肥胖。在其15年的生命中，我们计划项目的核心已建造了一个组织库，该组织库由800多个人类动脉粥样硬化标本保存，用于各种分析技术和质量控制。根据Sukhova博士的监督，Eugenia Shvartz通常执行标本的回顾性质量控制。 正式手册。每年两到四个样本（1994-1999）将用于质量 巨噬细胞，SMC和内皮细胞的免疫染色。最近的测试表明，从2000年至2006年获得的冷冻切片的完美制备和质量。从组织样品中提取的蛋白质在2000、2001、2004、2005和2007年冻结，表现出相似的产率，即5.5 -11pg/mg干重的干重与存储时间无关（-80？C）。该核心还将测量大多数项目实验设计不可或缺的几个等离子体成分。在其一生中，这个核心支持了一百多个出版物。