Small Animal Core: Models of Acute Lung Injury

小动物核心：急性肺损伤模型

• 批准号: 8214994
• 负责人: George Parsons Langan
• 金额: $ 30.06万
• 依托单位: UNIVERSITY OF ILLINOIS AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-02-01 至 2013-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8214994
• 关键词: Acute Lung Injury; Alveolus; Animal Model; Animals; Basic Science; Biological Markers; Biology; Blood Vessels; Canis familiaris; Caring; Cell model; Chicago; Clinical; Clinical Medicine; Collaborations; Data; Data Analyses; Disease; Edema; Ensure; Equipment; Evaluation; Event; Evolution; Functional disorder; Gases; Genetically Engineered Mouse; Housing; Human Resources; Hyperoxia; Image; Inflammation; Intervention; Investigation; Lung; Magnetic Resonance Imaging; Measurement; Mechanics; Mediator of activation protein; Microscopic; Microsurgery; Modeling; Molecular; Mus; Organ; Oxygen; Permeability; Physiological; Physiology; Process; Protocols documentation; Publishing; Rattus; Regulation; Research; Research Personnel; Research Project Grants; Resources; Techniques; Therapeutic; Therapeutic Agents; Tissues; Training; Transgenic Mice; Translations; Universities; Vascular Permeabilities; Ventilator; animal care; base; clinically relevant; experience; in vivo; insight; laboratory facility; lung injury; mouse model; novel; novel therapeutics; optical imaging; oxygen toxicity; programs; research study; tool

The Small Animal Core: Models of Acute Lung Injury (ALI) will provide two major core functions to support the PPG's research investigators and five research projects. The first function is providing murine models of ALI (LPS- and vertilator-induced) with the core ensuring reproducible studies with a complete range of expertise and tools to obtain critical information pertaining to the cellular and molecular basis of endothelial barrier permeability. The Core, using well published techniques and parameters of acute lung injury, will employ state-of-the-art evaluation of pulmonary pathophysiology, interventions, data analysis, and interpretation, in order to provide insight into the efficacy and mechanisms of clinically relevant management approaches and to facilitate the translation of basic research to the clinical arena. The Core will take advantage of the resources available at the University of Chicago Small Animal Imaging core (which includes MRI, CT Optical Imaging, etc. This Core provides quantitative measurements of permeability, inflammation, edema, vascular leak and biomarkers. A second core function is to care for the wild type and geneticallyengineered mice utilized in this Program and to generate novel transgenic mice where indicated. Core leaders have assembled talented and well published personnel and enjoy newly built laboratory facilities to satisfy a wide range of experimental physiological and imaging needs. Core C personnel have professional experience spanning the fields of physiology, microsurgery, biology, and animal care and has already provided convincing evidence of coherent interactions with investigators of all five research projects delivering key preliminary results related to our PPG projects.

小动物核心：急性肺损伤(ALI)模型将提供两大核心功能支持 PPG的研究调查人员和五个研究项目。第一个功能是提供小鼠模型 ALI(内毒素和立枯剂诱导)，其核心确保可重复性研究，具有完整的 获取与内皮细胞和分子基础有关的关键信息的专业知识和工具 屏障渗透性。核心，使用已公布的急性肺损伤的技术和参数，将 采用最先进的肺部病理生理学评估、干预、数据分析和 解释，以提供对临床相关治疗的有效性和机制的洞察 并促进基础研究向临床领域的转化。核心将采取 芝加哥大学小动物影像中心现有资源的优势(包括 MRI、CT光学成像等。该核心提供渗透性、炎症、 浮肿、血管渗漏和生物标志物。第二个核心功能是照顾野生的和基因工程的 在本计划中使用的小鼠，并在指定的情况下产生新的转基因小鼠。堆芯 领导者聚集了才华横溢的出版人员，并享受新建的实验室设施，以 满足广泛的实验生理和成像需求。核心C级人员拥有专业的 在生理学、显微外科、生物学和动物护理领域拥有丰富的经验，已经 提供了令人信服的证据，表明与所有五个研究项目的研究人员进行了连贯的互动 提供与我们的PPG项目相关的关键初步成果。