Nanopatch System for Next Generation Ion Channel Recordings

用于下一代离子通道记录的 Nanopatch 系统

• 批准号: 8332307
• 负责人: Geoffrey Barrall
• 金额: $ 35.79万
• 依托单位: ELECTRONIC BIOSCIENCES, INC.
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-15 至 2014-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8332307
• 关键词: Cardiac; Disease; Dose; Drug Delivery Systems; Electronics; Frequencies; Individual; Ion Channel; Kinetics; Knowledge; Lipid Bilayers; Measurement; Medical; Membrane; Methods; Nerve; Noise; Performance; Phase; Physiological; Protocols documentation; Quartz; Regulation; Research Personnel; Resolution; Science; Small Business Innovation Research Grant; System; Tea; Temperature; Time; Wit; design; improved; nanopore; nanoscale; next generation; patch clamp; pressure; programs; prototype

DESCRIPTION (provided by applicant): This Phase II SBIR program aims to further develop Electronic Bio Sciences' (EBS) Nanopatch system for ion channel measurements. The proposed system will allow high sensitivity measurements previously only attainable with the patch clamp method to be performed on a planar lipid bilayer (PLB) system. The system relies on a nanometer scale bilayer on the quartz nanopore membrane platform and EBS designed electronics to reduce the noise typically associated with a traditional PLB system. The aims of this program are to improve the low frequency noise performance to rival that of patch clamping, adapt established protocols and develop new methods to increase the types of ion channels that can be incorporated into the Nanopatch system and demonstrate the capabilities of the system using an intracellular channel with low conductance and fast kinetics. Four prototype Nanopatch systems will also be produced. This improved Nanopatch system will have several distinct advantages over currently available ion channel measurement apparatus including a low noise, high bandwidth platform, robust and reproducible bilayer formation, fine temperature and pressure control and automated bilayer formation and ion channel incorporation. The proposed Nanopatch system will enable a large range of low conductance channels and channels with fast kinetics to be evaluated in a PLB at previously unattainable resolution and sensitivity.

描述（由申请人提供）：该 II 期 SBIR 计划旨在进一步开发用于离子通道测量的电子生物科学 (EBS) Nanopatch 系统。所提出的系统将允许在平面脂质双层（PLB）系统上进行以前只能通过膜片钳方法才能实现的高灵敏度测量。该系统依靠石英纳米孔膜平台上的纳米级双层和 EBS 设计的电子器件来降低通常与传统 PLB 系统相关的噪声。该计划的目的是提高低频噪声性能，以与膜片钳相媲美，调整现有方案并开发新方法来增加可纳入 Nanopatch 系统的离子通道类型，并展示该系统使用低电导和快速动力学的细胞内通道的功能。还将生产四个 Nanopatch 原型系统。与目前可用的离子通道测量装置相比，这种改进的 Nanopatch 系统将具有几个明显的优势，包括低噪声、高带宽平台、稳健且可重复的双层形成、精细的温度和压力控制以及自动双层形成和离子通道合并。所提出的 Nanopatch 系统将使大范围的低电导通道和具有快速动力学的通道能够在 PLB 中以以前无法达到的分辨率和灵敏度进行评估。