Flow Cytometry Core: Core 2

流式细胞术核心摘要： 流式细胞仪核心实验室(FCCL)是在Cobre计划第一阶段结束时启动的。最常用的两台仪器是通过私人捐赠购买的，科布雷提供了资金来雇用操作员、维护设备和购买必要的消耗品。最初，FCCL为Cobre资助的调查人员提供服务，但很快发展成为KUMC研究社区基础设施的重要组成部分。在过去的一年里，该核心已经为堪萨斯城地区的72名调查人员提供了服务。随着FCCL的用户基础扩大，人员配备和仪器设备也增加了。我们增加了第二台流式细胞仪、Luminex、激光扫描显微镜、Calio和RoboSep。我们还增加了一个生物安全室，在那里我们可以FACS-纯化人类和非人类灵长类细胞。此外，我们增加了两名人员：一名经理和一名流式细胞仪技术人员。我们成立了内部流动咨询委员会(IFAC)和外部流动咨询委员会(EFAC)，以监督FCCL在我们向可持续商业模式迈进的过程中取得的进展。 调查人员使用FCCL的程序以指导和培训为中心。我们指导研究人员使用最好的技术来解决他们的科学问题。我们还就成功完成实验所需的最佳试剂组合向调查人员提供咨询。一旦用户准备好进行实验，我们就建议他们关于实验技术。然后，我们对每个用户进行有关设备操作的单独培训。一旦获得数据，我们就训练用户分析和解释数据。当研究人员完成FCCL的体验后，他们明白为什么所使用的技术对他们的生物学问题是最好的，以及如何准备样本、获取数据、分析数据和呈现数据。

Flow Cytometry Core Abstract: The flow cytometry core laboratory (FCCL) was initiated at the end of the phase I of the COBRE program. The two instruments that are used most often were purchased through a private donation and COBRE provided the funds to employ an operator, maintain the equipment, and purchase the necessary consumables. Initially, the FCCL served COBRE-funded investigators, but quickly grew to be an essential component of the infrastructure of the KUMC research community. The core has served 72 investigators in the Kansas City area during the past year. As the user base of the FCCL has grown, so has the staffing and instrumentation. We added a second flow cytometer, Luminex, laser scanning microscope, Celigo, and RoboSep. We also added a biosafety room in which we can FACS-purify human and non-human primate cells. In addition, we added two personnel: a manager and a flow cytometry technician. We established an internal flow advisory committee (IFAC) and an external flow advisory committee (EFAC) to monitor the progress of the FCCL as we progress toward a sustainable business model. Procedures for investigators to use the FCCL are centered around mentoring and training. We mentor investigators as to the best technology available to address their scientific questions. We also counsel investigators as to the best combination of reagents available to successfully complete the experiments. Once users are ready to perform the experiments, we advise them regarding experimental technique. Then, we train each user individually in the operation of the relevant equipment. Once data are obtained, we train the user to analyze and interpret the data. At the completion of the investigator's experience with the FCCL, they understand why the technology used is the best for their biological question, how to prepare the samples, acquire the data, analyze the data, and present the data.