Flow Cytometry Core: Core 2

流式细胞术核心摘要： 流式细胞术核心实验室（FCCL）在COBRE项目I期结束时启动。最常用的两种仪器是通过私人捐赠购买的，COBRE提供了雇用操作员、维护设备和购买必要消耗品的资金。最初，FCCL服务于COBRE-funded研究人员，但很快就成长为KUMC研究社区基础设施的重要组成部分。在过去的一年里，该中心为堪萨斯城地区的72名调查人员提供了服务。随着FCCL用户群的增长，人员配备和仪器也在增加。我们增加了第二台流式细胞仪Luminex、激光扫描显微镜Celigo和RoboSep。我们还增加了一个生物安全室，在那里我们可以用流式细胞仪纯化人类和非人类灵长类动物的细胞。此外，我们增加了两名人员：一名经理和一名流式细胞术技术员。我们成立了内部流动咨询委员会（IFAC）和外部流动咨询委员会（EFAC），以监督FCCL的进展，因为我们正在向可持续的商业模式迈进。 调查人员使用FCCL的程序以指导和培训为中心。我们指导研究人员使用最好的技术来解决他们的科学问题。我们还为研究人员提供咨询，以确定成功完成实验的最佳试剂组合。一旦用户准备好进行实验，我们就实验技术向他们提供建议。然后，我们对每个用户单独进行相关设备的操作培训。一旦获得数据，我们就训练用户分析和解释数据。在完成研究者的经验与FCCL，他们明白为什么所使用的技术是最好的为他们的生物问题，如何准备样本，获取数据，分析数据，并提出数据。

Flow Cytometry Core Abstract: The flow cytometry core laboratory (FCCL) was initiated at the end of the phase I of the COBRE program. The two instruments that are used most often were purchased through a private donation and COBRE provided the funds to employ an operator, maintain the equipment, and purchase the necessary consumables. Initially, the FCCL served COBRE-funded investigators, but quickly grew to be an essential component of the infrastructure of the KUMC research community. The core has served 72 investigators in the Kansas City area during the past year. As the user base of the FCCL has grown, so has the staffing and instrumentation. We added a second flow cytometer, Luminex, laser scanning microscope, Celigo, and RoboSep. We also added a biosafety room in which we can FACS-purify human and non-human primate cells. In addition, we added two personnel: a manager and a flow cytometry technician. We established an internal flow advisory committee (IFAC) and an external flow advisory committee (EFAC) to monitor the progress of the FCCL as we progress toward a sustainable business model. Procedures for investigators to use the FCCL are centered around mentoring and training. We mentor investigators as to the best technology available to address their scientific questions. We also counsel investigators as to the best combination of reagents available to successfully complete the experiments. Once users are ready to perform the experiments, we advise them regarding experimental technique. Then, we train each user individually in the operation of the relevant equipment. Once data are obtained, we train the user to analyze and interpret the data. At the completion of the investigator's experience with the FCCL, they understand why the technology used is the best for their biological question, how to prepare the samples, acquire the data, analyze the data, and present the data.