METAGENOMIC ANALYSIS OF AN AQUATIC ENVIRONMENT AT THE LOWELL/EDEN ASBESTOS MINES

洛厄尔/伊登石棉矿水生环境的宏基因组分析

• 批准号: 8360434
• 负责人: ELIZABETH D DOLCI
• 金额: $ 3.09万
• 依托单位: UNIVERSITY OF VERMONT & ST AGRIC COLLEGE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8360434
• 关键词: Address; Asbestos; Beds; Communities; Complex; County; DNA; Ecosystem; Environment; Funding; Future; Genes; Genetic; Goals; Grant; Habitats; Health; Heavy Metals; Human; Investigation; Libraries; Metagenomics; Microbiology; Mining; National Center for Research Resources; Play; Principal Investigator; Proteins; Proteomics; Research; Research Infrastructure; Resources; Risk; Role; Site; Source; Stream; Stress; Toxin; United States National Institutes of Health; Vermont; Water; cDNA Library; cost; microbial community; microorganism

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The emerging fields of metagenomics and proteomics are significantly transforming our understanding of the roles microorganisms play in all ecosystems, from the human biome to toxin laden environments. Microbiology is no longer restricted to analysis of pure cultures of one species, isolated from natural habitats and interactions with other microflora. The long range goal of this investigation is to assess the microbial community of a complex, environmentally-stressed site within Lamoille County, Vermont. The Vermont Asbestos Group Mine site, located in Lowell and Eden is impacting both the environmental and human health of the region (Levy, 2008; Vermont Dept of Health, 2008). Water and stream bed quality are contaminated with heavy metals, elevated pH levels, and asbestos-laden mine tailings. an A health advisory indicates increased potential risk to humans of asbestos-related illnesses. The objectives of the project are to (a) construct a metagenomic library of community DNA from an impacted aquatic environment at the Mine site, (b) create a cDNA library of expressed genes of the microorganisms inhabiting the site, and (c) isolate total protein from the microbial community for future proteomic analysis. This integrative approach will enable us to address the dynamic roles microorganisms play in complex, human-impacted environments.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其他NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 宏基因组学和蛋白质组学的新兴领域正在显著改变我们对微生物在所有生态系统中所起作用的理解，从人类生物群系到充满毒素的环境。微生物学不再局限于分析一个物种的纯培养物，从自然栖息地隔离，并与其他微生物区系的相互作用。本次调查的长期目标是评估佛蒙特州拉莫伊尔县内一个复杂的、环境压力大的场地的微生物群落。位于洛厄尔和伊登的佛蒙特州阿斯彭集团矿场正在影响该地区的环境和人类健康（Levy，2008年;佛蒙特州卫生部，2008年）。水和河床质量受到重金属、pH值升高和含石棉尾矿的污染。a健康咨询表明，人类患石棉相关疾病的潜在风险增加。该项目的目标是：（a）从矿区受影响的水生环境中构建一个群落DNA宏基因组文库;（B）创建一个栖息在矿区的微生物表达基因cDNA文库;（c）从微生物群落中分离总蛋白质，用于未来的蛋白质组分析。这种综合方法将使我们能够解决微生物在复杂的、受人类影响的环境中发挥的动态作用。