CENTRAL RESEARCH SERVICES CORE UNLV

UNLV 中央研究服务核心

• 批准号: 8360607
• 负责人: RONALD K GARY
• 金额: $ 20.15万
• 依托单位: UNIVERSITY OF NEVADA RENO
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8360607
• 关键词: Biological Sciences; Biomedical Research; Charge; Commit; Detection; Devices; Discipline; Electrophoresis; Ensure; Equipment; Flow Cytometry; Fluorescence; Funding; Gel; Genomics; Grant; Healthcare; Image; Laboratories; Learning; Molecular; National Center for Research Resources; Occupations; Principal Investigator; Process; Property; Proteins; Proteomics; Reader; Research; Research Infrastructure; Research Personnel; Resources; Reverse Transcriptase Polymerase Chain Reaction; Sampling; Scanning; Scientist; Services; Solutions; Source; System; Techniques; Time; Training; United States National Institutes of Health; cost; design; gene function; instrument; instrumentation; meetings; member; operation; two-dimensional

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The UNLV Central Research Services Core is designed to aid researchers in studying the properties and functions of genes and their products. This facility provides investigators with access to essential multi-user equipment and high quality research services. The laboratory also serves as a training platform for scientists at all levels wishing to learn genomic and proteomic techniques. The laboratory staff maintain the facility to ensure safe and reliable operation of all instruments. The Core staff are committed to helping scientists from all disciplines in life science to take advantage of the available research services, and they provide individualized assistance to meet non-routine needs. Available services include microarray, quantitative real-time PCR, gel and blot imaging (via storage phosphor, fluorescence scanning, and chemiluminescence detection), protein electrophoresis (one- and two-dimensional), and flow cytometry. Specific instrumentation within the Central Research Services Core includes a BioRad iCycler RT-PCR machine, a Becton & Dickinson FACSCalibur flow cytometer, a GE Healthcare variable-mode imager (Typhoon), a Bio-Rad Experion Automated Electrophoresis System, a Molecular Devices Spectramax 386plus plate reader, a NanoDrop ND-1000 Spectrophotometer, a Agilent Bioanalyzer, a Genomics Solutions Hyb4 station, a Molecular Devices GenePix 4000B scanner, a Affymetrix GeneChip Instrumentation System, and a GeneMachines Omnigrid 300 printer. Depending on the job, instruments are used directly by customers (with hourly usage charges) or customer samples are processed by Core technical staff members with labor rates included in the re-charge calculation..

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 UNLV中央研究服务核心旨在帮助研究人员研究基因及其产物的性质和功能。该设施为研究人员提供了基本的多用户设备和高质量的研究服务。 该实验室还为希望学习基因组学和蛋白质组学技术的各级科学家提供培训平台。 实验室工作人员维护设施，以确保所有仪器的安全可靠运行。 核心工作人员致力于帮助来自生命科学各个学科的科学家利用现有的研究服务，他们提供个性化的援助，以满足非常规需求。 可用的服务包括微阵列、定量实时PCR、凝胶和印迹成像（通过存储磷光体、荧光扫描和化学发光检测）、蛋白质电泳（一维和二维）和流式细胞术。 中央研究服务核心内的特定仪器包括BioRad iCycler RT-PCR机器、Becton & Dickinson FACSCalibur流式细胞仪、GE Healthcare可变模式成像仪（Typhoon）、Bio-Rad Experion自动电泳系统、Molecular Devices Spectramax 386 plus板读取器、NanoDrop ND-1000分光光度计、Agilent生物分析仪、Genomics Solutions Hyb 4站、Molecular Devices GenePix 4000 B扫描仪、Affyphidae GeneChip仪器系统和GeneMachines Omnigrid 300打印机。 根据工作的不同，仪器由客户直接使用（按小时收费），或者客户样品由核心技术人员处理，人工费包含在再收费计算中。