COBRE: UID: PROJ 2: SPATIAL STRUCTURE AND ADAPTIVE EVOLUTION OF VIRUSES

COBRE：UID：项目 2：病毒的空间结构和自适应进化

• 批准号: 8359576
• 负责人: STEPHEN M. KRONE
• 金额: $ 21.66万
• 依托单位: UNIVERSITY OF IDAHO
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-02-01 至 2012-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8359576
• 关键词: Address; Affect; Agar; Applications Grants; Automobile Driving; Bacteriophages; Behavior; Biological; Biological Models; Centers of Research Excellence; Clinical; Communities; Data; Environment; Evolution; Experimental Models; Funding; Grant; Heterogeneity; Human; Investigation; Joints; Microbial Biofilms; Molecular; National Center for Research Resources; Population; Principal Investigator; Process; Research; Research Infrastructure; Resources; Role; Shapes; Source; Structure; Surface; System; Time; United States National Institutes of Health; Virus; cost; environmental change; mathematical model; microbial; microbial community; pathogen; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Most microbial communities in natural and clinical settings are spatially structured, appearing as surface-associated populations such as biofilms. This structure profoundly affects the ecological and evolutionary forces that shape the community. This is particularly important in the battle against ever-evolving human pathogens that have ecological and evolutionary time scales much shorter than human time scales. Despite this, very little is known about the specific effects of spatial heterogeneity on the adaptive evolution of microbial pathogens. Thus, a joint experimental and theoretical investigation is proposed to uncover fundamental details about the role of spatial structure in driving evolutionary and ecological dynamics of viruses. A simple experimental model system will provide a framework for addressing these issues. This experimental system will be used to study the molecular adaptive evolution of bacteriophages infecting their bacterial hosts on agar plates under changing environmental conditions. In conjunction with these experiments, new spatially explicit mathematical models (stochastic cellular automata) will be developed and fit to empirical observations with the aims of predicting the behavior of the microbial systems, uncovering the underlying biological mechanisms that are most important in the adaptive evolution of viruses, and generating new hypotheses. This project will focus on the impact of spatial structure on adaptive evolution to a single change in environment and will generate preliminary data for an independent R01 grant application that addresses adaptive evolution in the presence of fluctuating environmental conditions.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 自然和临床环境中的大多数微生物群落是空间结构化的，表现为表面相关的种群，如生物膜。这种结构深刻地影响了塑造社区的生态和进化力量。这在与不断进化的人类病原体的斗争中尤为重要，这些病原体的生态和进化时间尺度比人类的时间尺度短得多。尽管如此，很少有人知道空间异质性对微生物病原体适应性进化的具体影响。因此，提出了一个联合的实验和理论研究，以揭示空间结构在驱动病毒进化和生态动力学中的作用的基本细节。一个简单的实验模型系统将为解决这些问题提供一个框架。该实验系统将用于研究噬菌体在变化的环境条件下感染琼脂平板上的细菌宿主的分子适应性进化。与这些实验相结合，将开发新的空间明确的数学模型（随机细胞自动机），并将其与经验观察相适应，目的是预测微生物系统的行为，揭示病毒适应性进化中最重要的潜在生物学机制，并产生新的假设。该项目将重点关注空间结构对适应性进化的影响，以适应环境中的单一变化，并将为独立的R 01赠款申请产生初步数据，该申请涉及在波动的环境条件下的适应性进化。