Proteomics

蛋白质组学

• 批准号: 8379559
• 负责人: Martin Arthur Moseley
• 金额: $ 4.55万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8379559
• 关键词: Asses; Biological Markers; Cells; Charge; Clinical; Code; Communities; Complex; Computer software; Core Facility; Coupled; Data; Data Security; Data Storage and Retrieval; Databases; Digestion; Drug Kinetics; Gel; Genome; Genomics; Gold; Grant; Hybrids; Institutes; Ions; Isoelectric Focusing; Isotopes; Label; Laboratories; Licensing; Liquid Chromatography; Liquid substance; Malignant Neoplasms; Mass Spectrum Analysis; Monitor; Peptides; Performance; Preparation; Process; Progest; Proteins; Proteomics; Research; Resolution; Resources; Robot; Running; Sampling; Science; Science Policy; System; Technology; Time; Water; base; computerized data processing; data acquisition; ion mobility; mass analyzer; mass spectrometer; medical schools; member; molecular shape; nanoscale; pressure; research study

The School of Medicine, the Duke Comprehensive Cancer Institute, and the Institute for Genome Science & Policy have collaborated to create a Proteomics Core Facility to provide protein characterization resources for Duke Comprehensive Cancer Institute members and the entire Duke Research Gommunity. The Proteomics Core Facility (http://www.genome.duke.edu/cores/proteomics) is located in a ~1,900 sq. ft. laboratory in the Levine Science Research Center, and provides capabilities for mass spectrometry based proteomics for protein identification and protein quantitation, including biomarker discovery and biomarker verification experiments.' For qualitative identificafions and biomarker discovery experiments ('omic-scale qualitative and quantitative analyses), the laboratory is equipped with four high resolution accurate mass LC/MS/MS systems, each using a dedicated ultra-high performance nanoscale liquid chromatography systems (Waters NanoAcquity). Three ofthe MS/MS systems are hybrid quadruple time-of-fiight tandem mass spectrometers (Waters Q-Tof Ultima, Q-Tof Premier, and Synapt High Definition Mass Spectrometer). The fourth system is a hybrid linear ion trap - orbitrap system (Thermo's LTQ-Orbitrap). These systems can be used for label-free, gel-free' differential expression experiments, and also for isotope coding proteomics (such as SILAC or ITRAQ). For biomarker verification experiments, targeted mass spec quantitative experiments are performed on an ultra-high performance nanoscale LC system coupled to a triple quadrupole tandem mass spectrometer (Waters Quattro Premier). For these experiments, data acquisition is accomplished using LC/MS/MS with Mulfiple Reacfion Monitoring, a technology whiqh is the 'Gold Standard' for clinical pharmacokinetics studies. In addition, the Synapt High Definition: Mass Spectrometer (Q-Tof with an ion-mobility mass analyzer) provides for unique experiments characterizing piepfides and proteins not only on the basis of mass and charge, but also molecular shape/size, using a unique ionmobility cell located between the quadrupole and fime-of-fiight mass analyzers.

医学院、杜克综合癌症研究所和基因组科学研究所 Policy 合作创建了蛋白质组学核心设施，以提供蛋白质表征资源 面向杜克综合癌症研究所成员和整个杜克研究社区。这 蛋白质组学核心设施 (http://www.genome.duke.edu/cores/proteomics) 位于约 1,900 平方英尺的场地内。 莱文科学研究中心的实验室，并提供基于质谱的能力 用于蛋白质鉴定和蛋白质定量的蛋白质组学，包括生物标志物发现和生物标志物 验证实验。用于定性鉴定和生物标志物发现实验（“组学规模 定性和定量分析），实验室配备四台高分辨率精确质量 LC/MS/MS 系统，每个系统均使用专用的超高性能纳米级液相色谱 系统（沃特世 NanoAcquity）。其中三个 MS/MS 系统是混合四重飞行时间串联 质谱仪（Waters Q-Tof Ultima、Q-Tof Premier 和 Synapt 高清晰度质谱仪）。 第四个系统是混合线性离子阱-orbitrap系统（Thermo的LTQ-Orbitrap）。这些系统可以 可用于无标记、无凝胶差异表达实验，也可用于同位素编码蛋白质组学 （例如 SILAC 或 ITRAQ）。对于生物标志物验证实验，目标​​质谱定量 实验是在超高性能纳米级 LC 系统上进行的，该系统耦合到三重 四极串联质谱仪（Waters Quattro Premier）。对于这些实验，数据采集 是使用 LC/MS/MS 和多重反应监测来完成的，这项技术被称为“黄金技术” 临床药代动力学研究的标准。此外，Synapt High Definition：质谱仪 （带有离子淌度质量分析仪的 Q-Tof）提供了表征 Piepfides 和 蛋白质不仅基于质量和电荷，还基于分子形状/大小，使用独特的离子淌度 池位于四极杆和飞行时间质量分析器之间。