Proteomics & Metabolomics Core

蛋白质组学

• 批准号: 10323310
• 负责人: Martin Arthur Moseley
• 金额: $ 9.19万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-01-01 至 2024-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10323310
• 关键词: Academy; Acetylation; Amino Acids; Amplifiers; Basic Science; Belief; Bile Acids; Biogenic Amines; Biological Assay; Biological Markers; Blood Platelets; Cancer Center Support Grant; Cell Line; Ceramides; Cholesterol Esters; Clinical Research; Collaborations; Computational Biology; Computer software; Consultations; Custom; Data; Data Analyses; Data Files; Development; Diglycerides; Dissociation; Doctor of Philosophy; Eicosanoids; Electrons; Ensure; Equipment; Experimental Designs; Fingerprint; Funding; Generations; Genomics; Guidelines; Hydroxycholesterols; Institutes; Journals; Label; Laboratories; Lecithin; Leukocytes; Lipids; Liquid Chromatography; Lysophosphatidylcholines; Malignant Neoplasms; Management Information Systems; Mass Spectrum Analysis; Methods; Monoclonal Antibodies; Online Systems; Output; Performance; Phosphorylation; Plasma; Post-Translational Protein Processing; Postdoctoral Fellow; Preparation; Protein Analysis; Protein Footprinting; Proteins; Proteomics; Proteomics Shared Resource; Protocols documentation; Publications; Reporting; Research; Research Institute; Research Personnel; Resolution; Resource Sharing; Resources; Sampling; Scientist; Secure; Serum; Services; Sphingomyelins; Statistical Data Interpretation; Synovial Fluid; System; Technology; Time; Tissues; Translations; Triglycerides; Ubiquitination; Urine; Vendor; Volatile Fatty Acids; Water; Work; acylcarnitine; base; biomarker discovery; clinical practice; cost; cost effective; crosslink; data integrity; data quality; data repository; design; differential expression; experience; experimental study; falls; industry partner; innovation; instrument; instrumentation; long chain fatty acid; mass spectrometer; meetings; member; metabolomics; professor; programs; protein complex; repository; research study; square foot; tumor

ABSTRACT – PROTEOMICS AND METABOLOMICS SHARED RESOURCE The DCI Proteomics and Metabolomics Shared Resource (DPM) provides the wide variety of mass spectrometry-based untargeted (‘omic) and targeted qualitative and quantitative capabilities for proteomics and for targeted metabolomics which are needed to support basic science studies. The lab also has the scale needed for support of clinical research studies, including biomarker discovery and biomarker verification experiments. The DPM is equipped with seven high-resolution mass spectrometers, three triple-quadrupole mass spectrometers, and eleven Ultra Performance Liquid Chromatography (UPLC) systems. This equipment includes four state-of-the-art mass spectrometers purchased within the past 18 months – a Fusion Lumos Tribrid with Electron Transform Dissociation (Thermo), Fusion Lumos Tribrid with Electron Transform Dissociation and UV Photodissociation (Thermo), a Q Exactive HF-X (Thermo) and a TQ-XS (Waters). The DPM has a full-time staff of eleven scientists, including six PhDs (four Research Professors, one scientific staff member and one Post- Doc). This group has over 195 years’ combined experience in mass spectrometry and more than 379 authored or co-authored publications (counting publications with two DMP members as co-authors as two author- publications). The resource is located in a new (2017) ~8,100 sq. ft. laboratory in the Chesterfield Building in downtown Durham, custom built for mass spectrometry-based proteomics and metabolomics. By working closely with vendors to obtain extensive discounts, prudent use of DPM Betterments Funds towards the new equipment, and the DCI CCSG support, the DPM has maintained cost-effective (revenue neutral) support for the DCI at state-of-the-art levels. The DPM’s objective is to generate high-quality data by providing full service to its customers. This includes initial consultations to create optimal experimental designs, use of rigorous QC protocols and standards from sample preparation through sample analyses, and generation of detailed reports to ensure clarity on experimental approach and results. Use of a Laboratory Information Management System (LIMS) ensures sample integrity (unique Project Numbers and Sample IS numbers automatically assigned by the LIMS), and the use of a true ‘fingerprinted’ data repository (“GCB Express Data Repository”) ensures data integrity. In 2018, the DPM provided services to 95 DCI members (41% of unique users), who accounted for 17% of total usage and represented 7 of the 8 DCI Research Programs. Use of this shared resource by DCI Members contributed to 65 publications over the project period, 19 of which were in high impact journals (IF>9), demonstrating the value of services offered by the resource. During this time, the DPM received support from the DCI CCSG and Institutional Support from the SoM in the form of new lab costs (design and build of new facility in the Chesterfield building) and new instrumentation (three state-of-the-art mass spectrometers). R:\2019 Competing Renewal\Shared Resources\Proteomics and Metabolomics\FINAL Documents\P&M Abstract Final Review 1.14.19.docx

摘要-蛋白质组学和代谢组学共享资源 DCI Proteomics and Metabolomics Shared Resource（DCI蛋白质组学和代谢组学共享资源）提供了各种质量 基于光谱的非靶向（'omic）和靶向的蛋白质组学定性和定量能力， 用于支持基础科学研究所需的靶向代谢组学。实验室也有所需的规模 用于支持临床研究，包括生物标志物发现和生物标志物验证实验。 该仪器配备了七个高分辨率质谱仪，三个三重四极杆质谱仪， 光谱仪和11个超高效液相色谱（UPLC）系统。该设备包括 在过去18个月内购买了四台最先进的质谱仪-Fusion Lumos Tribrid， 电子转换解离（Thermo）、Fusion Lumos Tribrid与电子转换解离和UV 光解（Thermo）、Q Exactive HF-X（Thermo）和TQ-XS（沃茨）。该委员会有一个全职工作人员 11名科学家，包括6名博士（4名研究教授，1名科学工作人员和1名博士后）， Doc）。该小组在质谱分析方面拥有超过195年的综合经验， 或共同撰写的出版物（将两名共同作者成员作为共同作者的出版物计算为两名作者- 出版物）。该资源位于一个新的（2017年）~ 8，100平方米。英尺位于英国伦敦的切斯特菲尔德大楼 位于达勒姆市中心，专为基于质谱的蛋白质组学和代谢组学而定制。密切合作 与供应商合作，以获得广泛的折扣，谨慎地使用新设备的增值基金， 和中央情报局局长CCSG支持，中央情报局一直保持对中央情报局局长的成本效益（收入中立）支持， 最先进的水平数据库的目标是通过向其提供全面服务来生成高质量的数据。 客户这包括初步咨询，以创建最佳的实验设计，使用严格的QC 从样品制备到样品分析的方案和标准，以及详细报告的生成 确保实验方法和结果的清晰度。实验室信息管理系统的使用 （LIMS）确保样品完整性（唯一的项目编号和样品IS编号由 LIMS），并使用真正的“指纹”数据存储库（“GCB Express数据存储库”）确保数据 完整 2018年，该公司为95个DCI成员（占唯一用户的41%）提供服务，占全球17%。 总使用量，代表了8个DCI研究项目中的7个。DCI成员使用此共享资源 在项目期间发表了65篇论文，其中19篇发表在高影响力期刊上（IF>9）， 展示资源提供的服务的价值。在此期间，该组织得到了来自 DCI CCSG和SoM以新实验室成本的形式提供的机构支持（设计和建造新的 在切斯特菲尔德大楼的设施）和新的仪器（三个国家的最先进的质谱仪）。 R：\2019竞争更新\共享资源\蛋白质组学和代谢组学\最终文件\P&M 摘要最终评审1.14.19.docx