Proteomics

医学院、杜克综合癌症研究所和基因组科学研究所 政策已经合作创建了一个蛋白质组学核心设施，以提供蛋白质表征资源 为杜克综合癌症研究所的成员和整个杜克研究所提供服务。的 蛋白质组学核心设施（http：//www.genome.duke.edu/cores/proteomics）位于约1，900平方米。英尺 Levine科学研究中心的实验室，并提供基于质谱的能力 用于蛋白质鉴定和蛋白质定量的蛋白质组学，包括生物标志物发现和生物标志物 验证实验用于定性鉴定和生物标志物发现实验（“组学规模 定性和定量分析），实验室配备了四个高分辨率的精确质量 LC/MS/MS系统，每个系统都使用专用的超高性能纳米级液相色谱 系统（沃茨NanoAcquity）。三个MS/MS系统是混合四重飞行时间串联 质谱仪（沃茨Q-Tof Ultima、Q-Tof Premier和Synapt高清晰度质谱仪）。 第四个系统是混合线性离子阱-轨道阱系统（Thermo的LTQ-Orbitrap）。这些系统可以 可用于无标记、无凝胶的差异表达实验，也可用于同位素编码蛋白质组学 (such如SILAC或ITRAQ）。对于生物标志物验证实验，靶向质谱定量 实验是在一个超高性能纳米LC系统耦合到三重 四极串联质谱仪（沃茨Quattro Premier）。对于这些实验，数据采集 使用LC/MS/MS与多重反应监测完成，这是一种“黄金”技术 临床药代动力学研究的标准。此外，Synapt高清晰度质谱仪 （具有离子迁移率质量分析仪的Q-Tof）提供了表征piepfides的独特实验， 蛋白质不仅基于质量和电荷，而且基于分子形状/大小，使用独特的离子迁移率 位于四极杆和飞行时间质量分析器之间的单元。

The School of Medicine, the Duke Comprehensive Cancer Institute, and the Institute for Genome Science & Policy have collaborated to create a Proteomics Core Facility to provide protein characterization resources for Duke Comprehensive Cancer Institute members and the entire Duke Research Gommunity. The Proteomics Core Facility (http://www.genome.duke.edu/cores/proteomics) is located in a ~1,900 sq. ft. laboratory in the Levine Science Research Center, and provides capabilities for mass spectrometry based proteomics for protein identification and protein quantitation, including biomarker discovery and biomarker verification experiments.' For qualitative identificafions and biomarker discovery experiments ('omic-scale qualitative and quantitative analyses), the laboratory is equipped with four high resolution accurate mass LC/MS/MS systems, each using a dedicated ultra-high performance nanoscale liquid chromatography systems (Waters NanoAcquity). Three ofthe MS/MS systems are hybrid quadruple time-of-fiight tandem mass spectrometers (Waters Q-Tof Ultima, Q-Tof Premier, and Synapt High Definition Mass Spectrometer). The fourth system is a hybrid linear ion trap - orbitrap system (Thermo's LTQ-Orbitrap). These systems can be used for label-free, gel-free' differential expression experiments, and also for isotope coding proteomics (such as SILAC or ITRAQ). For biomarker verification experiments, targeted mass spec quantitative experiments are performed on an ultra-high performance nanoscale LC system coupled to a triple quadrupole tandem mass spectrometer (Waters Quattro Premier). For these experiments, data acquisition is accomplished using LC/MS/MS with Mulfiple Reacfion Monitoring, a technology whiqh is the 'Gold Standard' for clinical pharmacokinetics studies. In addition, the Synapt High Definition: Mass Spectrometer (Q-Tof with an ion-mobility mass analyzer) provides for unique experiments characterizing piepfides and proteins not only on the basis of mass and charge, but also molecular shape/size, using a unique ionmobility cell located between the quadrupole and fime-of-fiight mass analyzers.