Development of a Novel Aerosol Mass Spectrometric Technology for Rapid and Sensit

开发快速灵敏的新型气溶胶质谱技术

• 批准号: 8433145
• 负责人: Manjula Canagaratna
• 金额: $ 24.67万
• 依托单位: AERODYNE RESEARCH, INC
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-09-20 至 2014-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8433145
• 关键词: Accounting; Address; Aerosols; Air Pollutants; Air Pollution; Airborne Particulate Matter; Archives; Asthma; Biological; Biological Markers; Biological Monitoring; Blood; Blood Volume; Blood specimen; California; Chemicals; Child; Childhood Asthma; Chromatography; Complex; Coupling; Detection; Development; Epidemiologic Studies; Epidemiology; Goals; Head; Health; Human; Human Resources; Human body; Individual; Infant; Institution; Laboratories; Link; Liquid substance; Location; Mass Spectrum Analysis; Measurement; Measures; Metabolic; Metals; Methods; Multivariate Analysis; Names; Outcome; Pattern; Phase; Pilot Projects; Poison; Principal Investigator; Process; Qi; Research; Resolution; Saliva; Sampling; Serum; Small Business Innovation Research Grant; Source; Techniques; Technology; Telephone; Testing; Time; Toxic Environmental Substances; Universities; Urine; Vulnerable Populations; analytical method; commercialization; data mining; design; environmental toxicology; insight; instrument; interest; mass spectrometer; meetings; new technology; novel; particle; planetary Atmosphere; pollutant; professor; prototype; toxicant

DESCRIPTION (provided by applicant): Development of a Novel Aerosol Mass Spectrometric Technology for Rapid and Sensitive Biomonitoring of Environmental Toxicants Name, address, and telephone number of the Principal Investigator: Dr. Manjula Canagaratna, Aerodyne Research, Inc., 45 Manning Rd., Billerica, MA 01821-3976, 1-978-663-9500 ext. 285 Names of other key personnel: Prof. Qi Zhang, Prof. Fumio Matsumura Participating institutions: Aerodyne Research Inc.; University of California, Davis This SBIR project will develop a novel technology for biomonitoring of environmental toxicants that cause adverse health effects in humans. The new technology will couple micro-nebulization with high resolution aerosol mass spectrometry to allow sensitive detection and chemical characterization of specific toxicants as well as toxicant classes in microvolumes of blood or biofluid samples. A droplet-on- demand (DOD) interface will convert microvolumes of liquids into aerosol particles of tightly controlled sizes and quantitatively deliver them into an aerosol mass spectrometer (AMS) for detection and chemical characterization of toxicants. DOD is a proven means of generating particles and aerosol mass spectrometry has been used for over a decade for sensitive and quantitative analysis of particle phase air pollutants with very fast time resolution (seconds to minutes). The coupling of DOD with AMS will allow characterization of liquid-phase species in real time, either directly in biological samples or after liquid chromatographic (LC) separation. The combined DOD-AMS instrument will be capable of simultaneously detecting and quantifying a wide variety of relevant toxic chemicals including inorganic, metal, and organic species. A prototype DOD interface will be developed and characterized in the laboratory with test aerosols containing known toxicants, and with archived blood samples from epidemiological studies. Data mining techniques will be developed to identify organizing patterns within the mass spectral distributions, understand correlations between detected species and health outcomes, and examine the association of multiple analytes and analyte classes with systemic effects. For the purpose of demonstrating the usefulness of this technology we will conduct an epidemiological pilot study on previously collected serum samples from asthmatic and non-asthmatic groups of children. We will first, simultaneously detect many toxicants or their metabolic products in the blood samples, second, use data mining techniques to characterize the toxicants, third identify the types and/or classes of toxicants in the serum that are most significantly associated with asthma using epidemiological assessment techniques. PUBLIC HEALTH RELEVANCE: This project addresses the critical need for new biomonitoring technology for rapid, sensitive, and simultaneous measurements of multiple known and suspected toxicants that can be ultimately linked with systematic effects in epidemiological and exposure studies. The proposed technology will allow sensitive detection and characterization of chemical species in small volumes of blood or other biofluids.

描述（由申请人提供）：开发用于环境毒物快速灵敏生物监测的新型气溶胶质谱技术主要研究者姓名、地址和电话号码：Dr. Manjula Canagaratna，Aerodyne Research，Inc.， 45 Manning Rd.，马萨诸塞州比勒利卡，邮编：01821-3976，电话：1-978-663-9500，分机：285其他关键人员姓名：张琦教授、松村文雄教授参与机构：Aerodyne Research Inc.;加州大学戴维斯分校该SBIR项目将开发一种新的技术，用于对人类造成不良健康影响的环境毒物进行生物监测。这项新技术将微雾化与高分辨率气溶胶质谱联用，以实现对微量血液或生物流体样品中特定毒物以及毒物类别的灵敏检测和化学表征。按需液滴（DOD）接口将微体积的液体转换为严格控制尺寸的气溶胶颗粒，并将其定量输送到气溶胶质谱仪（AMS）中，用于检测和化学表征有毒物质。DOD是一种经过验证的产生颗粒的方法，并且气溶胶质谱法已经被用于十多年，用于以非常快的时间分辨率（秒到分钟）对颗粒相空气污染物进行灵敏和定量分析。DOD与AMS的耦合将允许在真实的时间内直接在生物样品中或在液相色谱（LC）分离之后表征液相物质。 结合DOD-AMS仪器将能够同时检测和量化各种相关的有毒化学品，包括无机，金属和有机物种。将在实验室开发一个原型国防部接口，并使用含有已知毒物的测试气溶胶和流行病学研究中存档的血液样本进行表征。将开发数据挖掘技术，以确定质谱分布中的组织模式，了解检测到的物种和健康结果之间的相关性，并检查多种分析物和分析物类别与全身效应的关联。为了证明这项技术的有效性，我们将对以前从哮喘和非哮喘儿童群体中收集的血清样本进行流行病学初步研究。我们将首先，同时检测血液样本中的多种毒物或其代谢产物，其次，使用数据挖掘技术来表征毒物，第三，使用流行病学评估技术来识别血清中与哮喘最显著相关的毒物的类型和/或类别。 公共卫生相关性：该项目解决了对新的生物监测技术的迫切需要，以便快速、灵敏和同时测量多种已知和可疑的有毒物质，这些有毒物质最终可能与流行病学和接触研究中的系统性影响有关。拟议的技术将允许对少量血液或其他生物流体中的化学物质进行灵敏的检测和表征。