Multiplexed Onchip Nanomagnetic Immunoassay for TOxin Reporting (MONITOR)

描述(由申请人提供)：用于毒素报告的多路芯片纳米磁性免疫分析(监测)微/纳米工程技术，如芯片上实验室技术和纳米颗粒传感器，正在成为集成的高灵敏度、高通量的生物医学诊断的下一次革命工具。这些工具将使临床医生能够以方便、准确和非侵入性的护理点方式频繁、轻松地监测毒素或疾病，以便未来的医学研究和治疗。环境中发现的多种形式的二恶英和其他毒素相对不溶于水，但可溶于许多有机溶剂，尤其是可溶于脂肪组织。人体内的二恶英水平可以从血液样本中测定出来。使用传统方法，要测定二恶英血清水平，其准确度小于5ppt-TEQ，需要大量的毫升数量级的样本。因此，Biopico System和加州大学欧文分校提出了一种用于毒素报告(MONITOR)的多路芯片上纳米磁性免疫分析(MONITOR)，以检测血液中积累的多种毒素。这个新颖的护理点平台将使用100倍的血液作为诊断介质，并将血清分离流体与纳米磁阻免疫传感器相结合，在紧凑的分析仪器中快速量化多种毒素。这种小型化将使血清的简单、多路、高灵敏度、高特异性、集成的自动化快速全分析成为可能，与传统的分析设备相比，手持设备具有巨大的潜力。我们革命性的监测技术将采用快速血清分离技术以及带有电子读数的纳米颗粒耦合夹心分析来报告血液中的几种毒素，如果成功，将改变目前人类生物监测的范式。 公共卫生相关性：Biopico系统和加州大学欧文分校提出了一种用于毒素报告(监测器)的多路芯片上纳米磁性免疫分析方法，以检测血液中积累的多种毒素。该监测系统结合了免疫分析、血清分离微流控、纳米颗粒磁阻传感和声空化泵等技术，用于人体生物监测。这一新颖的护理点平台将使用血液作为诊断介质，并将最先进的微流体与磁阻纳米传感器相结合，在紧凑的分析仪器中快速量化口腔癌症。

DESCRIPTION (provided by applicant): Multiplexed Onchip Nanomagnetic Immunoassay for Toxin Reporting (MONITOR) Micro/Nano engineering techniques such as lab-on-a-chip technologies and nanoparticulates sensors are emerging as the next revolution tools for integrated high sensitive high throughput biomedical diagnostics. Such tools will enable clinicians to monitor toxins or diseases frequently and easily for future medical research and therapy in a convenient, accurate, and noninvasive point-of-care fashion. The many forms of dioxins and other toxins that are found in the environment are relatively insoluble in water but soluble in many organic solvents and especially soluble in the fatty tissues. Dioxin levels in the human body can be determined from a blood sample. Using traditional methods, to determine dioxin blood serum levels with an accuracy of less than 5 ppt-TEQ, a large sample size of the order of ml is required. Therefore, Biopico System and University of California, Irvine proposes a Multiplexed Onchip Nanomagnetic Immunoassay for Toxin Reporting (MONITOR) to detect multiple toxins accumulated in blood. This novel point-of-care platform will use 100 fold less blood as the diagnostic medium and bridge serum separation fluidics with nanomagnetoresistive immuno sensors for the rapid quantization of a multiple toxins in a compact analytical instrument. This miniaturization will enable simple, multiplexed, high sensitivity, high specificit, integrated automated rapid total analysis of serum and great potential for handheld device compared to conventional analytical devices. Our revolutionary MONITOR technology will employ rapid serum separation technique along with nanoparticle coupled sandwich assay with electronic readout for reporting several toxins in blood and when successful, will change the current paradigm of biomonitoring in humans. PUBLIC HEALTH RELEVANCE: Biopico System and UC Irvine propose a Multiplexed Onchip Nanomagnetic Immunoassay for TOxin Reporting (MONITOR) to detect multiple toxins accumulated in blood. This MONITOR system combines immunoassay, serum separation microfluidics, nanoparticle magneto resistive sensing and acoustic cavitation pump for biomonitoring in humans. This novel point-of-care platform will use blood as the diagnostic medium and bridge state-of-the-art microfluidics with magneto resistive nanosensors for the rapid quantization of an oral cancer in a compact analytical instrument.