Multiplexed Onchip Nanomagnetic Immunoassay for TOxin Reporting (MONITOR)

用于毒素报告的多重片上纳米磁性免疫分析 (MONITOR)

• 批准号: 8433149
• 负责人: John Collins
• 金额: $ 25万
• 依托单位: BIOPICO
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-09-20 至 2014-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8433149
• 关键词: Acoustics; Adipose tissue; Age; Aging; Anthracenes; Antibodies; Aroclor; Aroclors; Avidin; Benzo(a)pyrene; Binding; Biochemistry; Biological; Biological Assay; Biological Markers; Biological Monitoring; Biotin; Blood; Blood specimen; Buffers; California; Chemicals; Computer software; Coupled; Deposition; Detection; Devices; Diagnostic; Dioxins; Disease; Electronics; Environment; Enzyme-Linked Immunosorbent Assay; Evaluation; Film; Future; Goals; Head; Human; Human body; Immunoassay; Individual; Liquid substance; Magnetism; Measures; Medical Research; Methods; Microfluidics; Miniaturization; Monitor; Organic solvent product; Performance; Phase; Plasma; Polychlorinated Biphenyls; Process; Protocols documentation; Pump; Reaction; Reaction Time; Reagent; Reporting; Research; Sample Size; Sampling; Sensitivity and Specificity; Serum; Solutions; Specificity; Stream; Streptavidin; Surface; Suspension substance; Suspensions; System; Techniques; Technology; Testing; Toxin; Universities; Water; Whole Blood; aged; base; cost; data acquisition; design; diphenyl; innovative technologies; instrument; instrumentation; interest; iron oxide; magnetic field; malignant mouth neoplasm; micro-total analysis system; nanoengineering; nanolitre; nanomagnetic; nanoparticle; nanoparticulate; nanosensors; novel; operation; pentabromodiphenyl ether; point of care; programs; research study; sensor; tool

DESCRIPTION (provided by applicant): Multiplexed Onchip Nanomagnetic Immunoassay for Toxin Reporting (MONITOR) Micro/Nano engineering techniques such as lab-on-a-chip technologies and nanoparticulates sensors are emerging as the next revolution tools for integrated high sensitive high throughput biomedical diagnostics. Such tools will enable clinicians to monitor toxins or diseases frequently and easily for future medical research and therapy in a convenient, accurate, and noninvasive point-of-care fashion. The many forms of dioxins and other toxins that are found in the environment are relatively insoluble in water but soluble in many organic solvents and especially soluble in the fatty tissues. Dioxin levels in the human body can be determined from a blood sample. Using traditional methods, to determine dioxin blood serum levels with an accuracy of less than 5 ppt-TEQ, a large sample size of the order of ml is required. Therefore, Biopico System and University of California, Irvine proposes a Multiplexed Onchip Nanomagnetic Immunoassay for Toxin Reporting (MONITOR) to detect multiple toxins accumulated in blood. This novel point-of-care platform will use 100 fold less blood as the diagnostic medium and bridge serum separation fluidics with nanomagnetoresistive immuno sensors for the rapid quantization of a multiple toxins in a compact analytical instrument. This miniaturization will enable simple, multiplexed, high sensitivity, high specificit, integrated automated rapid total analysis of serum and great potential for handheld device compared to conventional analytical devices. Our revolutionary MONITOR technology will employ rapid serum separation technique along with nanoparticle coupled sandwich assay with electronic readout for reporting several toxins in blood and when successful, will change the current paradigm of biomonitoring in humans. PUBLIC HEALTH RELEVANCE: Biopico System and UC Irvine propose a Multiplexed Onchip Nanomagnetic Immunoassay for TOxin Reporting (MONITOR) to detect multiple toxins accumulated in blood. This MONITOR system combines immunoassay, serum separation microfluidics, nanoparticle magneto resistive sensing and acoustic cavitation pump for biomonitoring in humans. This novel point-of-care platform will use blood as the diagnostic medium and bridge state-of-the-art microfluidics with magneto resistive nanosensors for the rapid quantization of an oral cancer in a compact analytical instrument.

描述（由申请人提供）：用于毒素报告的多路芯片纳米磁免疫测定（MONITOR）微/纳米工程技术，如芯片实验室技术和纳米颗粒传感器正在成为集成高灵敏度高通量生物医学诊断的下一个革命工具。这些工具将使临床医生能够以方便、准确和无创的护理方式，频繁、轻松地监测毒素或疾病，以用于未来的医学研究和治疗。在环境中发现的许多形式的二恶英和其他毒素相对不溶于水，但可溶于许多有机溶剂，特别是可溶于脂肪组织。人体内的二恶英含量可以通过血液样本来测定。使用传统方法，测定血清二恶英水平的准确度低于5pt - teq，需要ml数量级的大样本量。因此，Biopico System和加州大学欧文分校提出了一种用于毒素报告的多路芯片纳米磁免疫测定法（MONITOR），以检测血液中积累的多种毒素。这种新型的即时护理平台将使用少100倍的血液作为诊断介质，并将血清分离流体与纳米磁阻免疫传感器连接起来，在紧凑的分析仪器中快速定量检测多种毒素。这种小型化将使简单、多路、高灵敏度、高特异性、集成自动化快速血清全分析成为可能，与传统分析设备相比，手持设备具有巨大的潜力。我们革命性的MONITOR技术将采用快速血清分离技术，以及纳米颗粒耦合夹心试验和电子读数来报告血液中的几种毒素，如果成功，将改变目前人类生物监测的范式。