1.19Hormonal controls of zebrafish post-embryonic melanocyte development

1.19斑马鱼胚胎后黑素细胞发育的激素控制

基本信息

  • 批准号:
    8208130
  • 负责人:
  • 金额:
    $ 5.22万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2010
  • 资助国家:
    美国
  • 起止时间:
    2010-01-27 至 2013-01-26
  • 项目状态:
    已结题

项目摘要

DESCRIPTION (provided by applicant): The long-term objective of this work is to identify genetic, cellular, and endocrine mechanisms required for post-embryonic development in vertebrates. This proposal focuses on metamorphosis of the zebrafish, which occurs between 12-28 days post-fertilization, and is analogous to human fetal and neonatal development. Among the many changes that occur during zebrafish metamorphosis is the transformation of an embryonic/early larval pigment pattern into that of the adult, a process that depends on the recruitment of latent stem cells and is likely under hormonal control. As thyroid hormone (TH) is critical for amphibian metamorphosis as well as mammalian post-embryonic development. Aim 1 will test roles for TH signaling in zebrafish somatic and pigment pattern metamorphosis. This will be accomplished using transgenic lines to block TH signals at specific times either throughout the fish or in specific cell lineages. To examine how global endocrine mediators effect local cellular processes of specification and morphogenesis, Aim 2 will test for interactions between TH signals and Wnt signals that are known to be critical for pigment cell 'development as well as stem cell self-renewal. These experiments will employ transgenic lines and other approaches to inhibit or stimulate these pathways and assay pathway activity and phenotypic outcomes. Finally, there may be many additional mediators of post-embryonic development, and to identify these, aim 3 will constitute a forward genetic screen for mutants that fail to undergo metamorphosis either entirely or have defects in the metamorphosis of specific traits and cell types. This screen will employ gene-breaking vectors and transposon-mediated insertional mutagenesis, allowing the efficient identification and analysis of affected loci. Together these efforts will provide significant new insights into the mechanisms of vertebrate post-embryonic development. *Vertebrates undergo extensive changes during postembryonic stages and many of these changes depend on the recruitment of differentiated progeny from self-renewing stem cell populations. Elucidating the genes, cell behaviors and hormonal mechanisms required for stem and progenitor cell renewal and differentiation is therefore important for understanding both human health and many disease syndromes and cancers.
描述(由申请人提供):这项工作的长期目标是确定脊椎动物胚胎后发育所需的遗传、细胞和内分泌机制。这项建议的重点是斑马鱼的变态,发生在受精后12-28天之间,类似于人类胎儿和新生儿的发育。在斑马鱼变态过程中发生的许多变化中,有一个是胚胎/早期幼虫的色素模式转变为成年人的色素模式,这一过程取决于潜伏干细胞的招募,并且可能受到激素的控制。甲状腺激素(TH)是两栖动物变态和哺乳动物胚后发育的关键激素。目的1探讨TH信号在斑马鱼体细胞和色素模式变态中的作用。这将通过使用转基因系在特定时间阻断整个鱼或特定细胞谱系中的TH信号来实现。为了研究全局内分泌介质如何影响局部细胞的特化和形态发生过程,Aim 2将测试TH信号和Wnt信号之间的相互作用,这些信号对色素细胞的发育以及干细胞的自我更新至关重要。这些实验将采用转基因系和其他方法来抑制或刺激这些途径,并测定途径活性和表型结果。最后,可能有许多额外的介质胚胎后的发展,并确定这些,目标3将构成一个正向遗传筛选突变体,不能完全经历变态或有缺陷的变态的特定性状和细胞类型。该筛选将采用基因断裂载体和转座子介导的插入诱变,从而有效地鉴定和分析受影响的基因座。这些努力将为脊椎动物胚胎后发育的机制提供重要的新见解。* 脊椎动物在胚后阶段经历广泛的变化,其中许多变化取决于从自我更新干细胞群体中招募分化的后代。因此,阐明干细胞和祖细胞更新和分化所需的基因、细胞行为和激素机制对于理解人类健康和许多疾病综合征和癌症都很重要。

项目成果

期刊论文数量(1)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Metamorphosis in teleosts.
{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

Sarah Kelly McMenamin其他文献

Sarah Kelly McMenamin的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('Sarah Kelly McMenamin', 18)}}的其他基金

Illuminating the mechanisms that generate pattern and shape during growth and regeneration of the zebrafish fin
阐明斑马鱼鳍生长和再生过程中产生图案和形状的机制
  • 批准号:
    10698173
  • 财政年份:
    2022
  • 资助金额:
    $ 5.22万
  • 项目类别:
Mechanisms of vertebrate post-embryonic developmental progression
脊椎动物胚胎后发育进程的机制
  • 批准号:
    9440774
  • 财政年份:
    2016
  • 资助金额:
    $ 5.22万
  • 项目类别:
Mechanisms of vertebrate post-embryonic developmental progression
脊椎动物胚胎后发育进程的机制
  • 批准号:
    9313275
  • 财政年份:
    2016
  • 资助金额:
    $ 5.22万
  • 项目类别:
Mechanisms of vertebrate post-embryonic developmental progression
脊椎动物胚胎后发育进程的机制
  • 批准号:
    9303491
  • 财政年份:
    2016
  • 资助金额:
    $ 5.22万
  • 项目类别:
Mechanisms of vertebrate post-embryonic developmental progression
脊椎动物胚胎后发育进程的机制
  • 批准号:
    8733740
  • 财政年份:
    2013
  • 资助金额:
    $ 5.22万
  • 项目类别:
Mechanisms of vertebrate post-embryonic developmental progression
脊椎动物胚胎后发育进程的机制
  • 批准号:
    8487482
  • 财政年份:
    2013
  • 资助金额:
    $ 5.22万
  • 项目类别:
1.19Hormonal controls of zebrafish post-embryonic melanocyte development
1.19斑马鱼胚胎后黑素细胞发育的激素控制
  • 批准号:
    7804866
  • 财政年份:
    2010
  • 资助金额:
    $ 5.22万
  • 项目类别:
1.19Hormonal controls of zebrafish post-embryonic melanocyte development
1.19斑马鱼胚胎后黑素细胞发育的激素控制
  • 批准号:
    8035282
  • 财政年份:
    2010
  • 资助金额:
    $ 5.22万
  • 项目类别:

相似海外基金

How Does Particle Material Properties Insoluble and Partially Soluble Affect Sensory Perception Of Fat based Products
不溶性和部分可溶的颗粒材料特性如何影响脂肪基产品的感官知觉
  • 批准号:
    BB/Z514391/1
  • 财政年份:
    2024
  • 资助金额:
    $ 5.22万
  • 项目类别:
    Training Grant
BRC-BIO: Establishing Astrangia poculata as a study system to understand how multi-partner symbiotic interactions affect pathogen response in cnidarians
BRC-BIO:建立 Astrangia poculata 作为研究系统,以了解多伙伴共生相互作用如何影响刺胞动物的病原体反应
  • 批准号:
    2312555
  • 财政年份:
    2024
  • 资助金额:
    $ 5.22万
  • 项目类别:
    Standard Grant
RII Track-4:NSF: From the Ground Up to the Air Above Coastal Dunes: How Groundwater and Evaporation Affect the Mechanism of Wind Erosion
RII Track-4:NSF:从地面到沿海沙丘上方的空气:地下水和蒸发如何影响风蚀机制
  • 批准号:
    2327346
  • 财政年份:
    2024
  • 资助金额:
    $ 5.22万
  • 项目类别:
    Standard Grant
Graduating in Austerity: Do Welfare Cuts Affect the Career Path of University Students?
紧缩毕业:福利削减会影响大学生的职业道路吗?
  • 批准号:
    ES/Z502595/1
  • 财政年份:
    2024
  • 资助金额:
    $ 5.22万
  • 项目类别:
    Fellowship
感性個人差指標 Affect-X の構築とビスポークAIサービスの基盤確立
建立个人敏感度指数 Affect-X 并为定制人工智能服务奠定基础
  • 批准号:
    23K24936
  • 财政年份:
    2024
  • 资助金额:
    $ 5.22万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Insecure lives and the policy disconnect: How multiple insecurities affect Levelling Up and what joined-up policy can do to help
不安全的生活和政策脱节:多种不安全因素如何影响升级以及联合政策可以提供哪些帮助
  • 批准号:
    ES/Z000149/1
  • 财政年份:
    2024
  • 资助金额:
    $ 5.22万
  • 项目类别:
    Research Grant
How does metal binding affect the function of proteins targeted by a devastating pathogen of cereal crops?
金属结合如何影响谷类作物毁灭性病原体靶向的蛋白质的功能?
  • 批准号:
    2901648
  • 财政年份:
    2024
  • 资助金额:
    $ 5.22万
  • 项目类别:
    Studentship
Investigating how double-negative T cells affect anti-leukemic and GvHD-inducing activities of conventional T cells
研究双阴性 T 细胞如何影响传统 T 细胞的抗白血病和 GvHD 诱导活性
  • 批准号:
    488039
  • 财政年份:
    2023
  • 资助金额:
    $ 5.22万
  • 项目类别:
    Operating Grants
New Tendencies of French Film Theory: Representation, Body, Affect
法国电影理论新动向:再现、身体、情感
  • 批准号:
    23K00129
  • 财政年份:
    2023
  • 资助金额:
    $ 5.22万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
The Protruding Void: Mystical Affect in Samuel Beckett's Prose
突出的虚空:塞缪尔·贝克特散文中的神秘影响
  • 批准号:
    2883985
  • 财政年份:
    2023
  • 资助金额:
    $ 5.22万
  • 项目类别:
    Studentship
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了