Mechanisms by which M. tuberculosis 13KDa lectin modulates human immune responses

结核分枝杆菌 13KDa 凝集素调节人体免疫反应的机制

基本信息

批准号：
8311544
负责人：
Andre Bafica
金额：
$ 5.31万
依托单位：
FEDERAL UNIVERSITY OF SANTA CATARINA
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-09-01 至 2013-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8311544
关键词：
Agonist Antibiotic Therapy Antigen-Presenting Cells Antitubercular Agents Bacteria Binding Biochemical Bioinformatics Biological Markers Blocking Antibodies Brazil C Type Lectin Receptors C-Type Lectins CD209 gene CD80 gene Cell Communication Cell Culture Techniques Cell Line Cell physiology Cells Cities Coculture Techniques Communicable Diseases Confocal Microscopy Cytokine Gene Cytometry Data Data Analyses Databases Dendritic Cells Development Diagnostic Disease Enzyme-Linked Immunosorbent Assay Erythrocytes Exposure to Family Flow Cytometry Gene Expression Genes Genus Mycobacterium Goals Green Fluorescent Proteins Health Host resistance Human Immune response Immune system Immunity Immunoglobulin G Immunologic Receptors Immunotherapy In Vitro Infection Inflammation Mediators Inflammatory Interferon Type II Interleukin-10 Interleukin-12 Interleukin-17 Interleukin-6 Knowledge Lectin Lung diseases MHC Class II Genes Macrophage-1 Antigen Measures Membrane Modeling Monoclonal Antibodies Mus Mycobacterium smegmatis Mycobacterium tuberculosis Outcome Outcome Study Pathogenesis Pathway interactions Patient Monitoring Patients Pattern Peripheral Blood Mononuclear Cell Phagocytosis Play Preparation Process Production Protein Binding Domain Proteins Publishing Reagent Recombinants Research Ricin Role Screening procedure Serum Surface System T-Cell Proliferation T-Lymphocyte TLR2 gene TLR3 gene TLR4 gene TLR7 gene TNF gene TNFRSF5 gene Testing Time Toll-like receptors Tuberculosis Virulence Work base chemotherapy cohort cytokine dectin 1 glucan phosphate immunoprophylaxis improved in vivo interest interleukin-23 laminaran macrophage monocyte mycobacterial neutralizing antibody novel pathogen protein protein interaction receptor receptor expression research study response stem sugar tool tuberculosis treatment uptake

项目摘要

Mechanisms by which M. tuberculosis 13 kDa lectin modulates human immune responses Project Summary Study of novel proteins from Mycobacterium tuberculosis may be a critical step for improving diagnostic tools, therapies and immunoprophylaxis against tuberculosis (TB), one of the most prevalent infectious diseases wordwide. We have identified a 13 KDa ricin-like lectin from M. tuberculosis that was found to agglutinate red blood cells and induce cytokine expression by macrophages. Moreover, serum from active tuberculosis patients contained high levels of IgG against this lectin. In the present proposal, we aim to characterize the role of 13 KDa Mtb lectin on human immune responses. The specific aims of the study are to 1) characterize the innate immune response profile elicited by the 13 KDa Mtb lectin; 2) determine innate receptor(s) expressed on human APC involved in the recognition of 13 KDa Mtb lectin; 3) investigate the role of 13 KDa Mtb lectin in the infection/phagocytosis process of M. tuberculosis by human APC and 4) examine specific humoral and cellular immune responses against 13 KDa Mtb lectin in TB patients before and after antibiotic treatment. Our long-term goal is to determine whether 13 KDa Mtb ricin-like lectin plays a role in regulating human immune responses to TB focusing on it possible activity in controlling APC function. These studies could be important in revealing a new target for immunotherapy of tuberculosis.

结核分枝杆菌13 kDa凝集素调节人类的机制免疫反应项目摘要研究结核分枝杆菌的新蛋白质可能是关键的步骤改善针对结核病（TB）的诊断工具，疗法和免疫预防，最普遍的传染病之一是Wordwide。我们已经确定了13 kDa 结核分枝杆菌的丽菌素样凝集素被发现凝集红细胞和通过巨噬细胞诱导细胞因子表达。此外，活性结核病的血清患者对此凝集素的IgG含量很高。在本提案中，我们的目标为了表征13 kDa MTB凝集素对人免疫反应的作用。具体该研究的目的是1）特征是引起先天免疫反应曲线由13 kDa mtb凝集素； 2）确定在人类上表达的先天受体 APC参与了13 kDa MTB凝集素的识别； 3）研究13的作用人类结核分枝杆菌的感染/吞噬作用过程中的KDA MTB凝集素 APC和4）检查针对13的特定体液和细胞免疫反应抗生素治疗前后结核病患者的KDA MTB凝集素。我们的长期目标是确定13 kDa mtb ricin样凝集素是否在调节人中起作用对结核病的免疫反应集中在控制APC功能时可能的活动。这些研究对于揭示新的免疫疗法的新靶标可能很重要结核。