Mechanisms by which M. tuberculosis 13KDa lectin modulates human immune responses

结核分枝杆菌13 kDa凝集素调控人类的机制 免疫反应 项目摘要 结核分枝杆菌新蛋白的研究可能是 改进结核病的诊断工具、治疗和免疫预防， 这是世界上最流行的传染病之一。我们已经确认了一只13 KDa的 结核分枝杆菌中的类似蓖麻毒素的凝集素，被发现能凝集红细胞和 巨噬细胞诱导细胞因子表达。此外，活动性肺结核患者的血清 患者体内有较高水平的抗该凝集素的抗体。在目前的提案中，我们的目标是 研究13 KDa Mtb凝集素在人体免疫应答中的作用。具体的 这项研究的目的是1)描述先天免疫反应的特征 13 KDa Mtb凝集素；2)人天然受体(S)的测定 APC参与13 KDa Mtb凝集素的识别；3)研究13 KDA Mtb凝集素在人感染/吞噬结核分枝杆菌过程中的作用 APC和4)检测针对13的特异性体液和细胞免疫反应 结核患者抗生素治疗前后KDA、Mtb、凝集素的变化我们的长期合作 目的是确定13 KDa的蓖麻毒素样凝集素是否在调节人类 结核的免疫反应侧重于它在控制APC功能中的可能活动。 这些研究可能对揭示免疫治疗的新靶点具有重要意义。 肺结核。

Mechanisms by which M. tuberculosis 13 kDa lectin modulates human immune responses Project Summary Study of novel proteins from Mycobacterium tuberculosis may be a critical step for improving diagnostic tools, therapies and immunoprophylaxis against tuberculosis (TB), one of the most prevalent infectious diseases wordwide. We have identified a 13 KDa ricin-like lectin from M. tuberculosis that was found to agglutinate red blood cells and induce cytokine expression by macrophages. Moreover, serum from active tuberculosis patients contained high levels of IgG against this lectin. In the present proposal, we aim to characterize the role of 13 KDa Mtb lectin on human immune responses. The specific aims of the study are to 1) characterize the innate immune response profile elicited by the 13 KDa Mtb lectin; 2) determine innate receptor(s) expressed on human APC involved in the recognition of 13 KDa Mtb lectin; 3) investigate the role of 13 KDa Mtb lectin in the infection/phagocytosis process of M. tuberculosis by human APC and 4) examine specific humoral and cellular immune responses against 13 KDa Mtb lectin in TB patients before and after antibiotic treatment. Our long-term goal is to determine whether 13 KDa Mtb ricin-like lectin plays a role in regulating human immune responses to TB focusing on it possible activity in controlling APC function. These studies could be important in revealing a new target for immunotherapy of tuberculosis.