ES Cell-Specific Genes and Reprogramming of Human Somatic Cells

ES细胞特异性基因和人类体细胞重编程

• 批准号: 8381277
• 负责人: Igor I. Slukvin
• 金额: $ 38.7万
• 依托单位: MORGRIDGE INSTITUTE FOR RESEARCH, INC.
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-08-01 至 2014-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8381277
• 关键词: Adult; Back; Bioinformatics; Cell Nucleus; Cell Transplantation; Cells; Clinical; Cloning; Data; Development; Epigenetic Process; Event; Gene Combinations; Gene Expression; Genes; Genetic; Genome; Hematopoietic; Human; Individual; Knock-in Mouse; Lead; Mesenchymal; Modeling; Modification; Myelogenous; Myeloid Cells; Oocytes; Patients; Phenotype; Process; Somatic Cell; Staging; System; Testing; Tissues; Trans-Activators; Undifferentiated; base; comparative; egg; embryonic stem cell; human embryonic stem cell; insight; novel; novel strategies; nuclear transfer; overexpression; pluripotency; regenerative therapy; research study; self-renewal

cells. We will perform microarray, epigenotyping, and bioinformatic analysis of these clones, fully reprogrammed "human ES cell"-like cells, wild type human ES cells, and mesenchymal and myeloid cells to offer insights into reprogramming to a pluripotent state and into cell fate changes to other lineages. This project will result in a significant improvement in our understanding of how the developmental status of differentiated cells can be altered. Reprogramming the genome from a somatic cell to a pluripotent state would be useful to generate patient-specific ES cells, thus overcoming the problem of tissue rejection resulting from genetic mismatches between donor and recipient. Perhaps more importantly, understanding how to reprogram differentiated cells to entirely new fates may ultimately lead to novel regenerative therapies that act on patients' endogenous cells in the absence of cell transplantation. This project represents an important early step in that direction.

细胞我们将对这些克隆进行微阵列、表观基因分型和生物信息学分析， 重编程的“人ES细胞”样细胞、野生型人ES细胞以及间充质和骨髓细胞 细胞提供洞察重编程到多能状态和细胞命运的变化， 血统 该项目将导致我们对发展如何理解的重大改进 可以改变分化细胞的状态。将基因组从体细胞重编程为 多能状态将有助于产生患者特异性ES细胞，从而克服该问题 捐赠者和接受者基因不匹配导致的组织排斥。也许更 重要的是，了解如何将分化的细胞重新编程为全新的命运， 从而产生了新的再生疗法， 移植该项目是朝着这一方向迈出的重要的早期步骤。