Transplantation of MHC Homozygous Vascular Progenitors in Primates

灵长类 MHC 纯合血管祖细胞移植

基本信息

  • 批准号:
    10181017
  • 负责人:
  • 金额:
    $ 115.49万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2016
  • 资助国家:
    美国
  • 起止时间:
    2016-09-20 至 2023-05-31
  • 项目状态:
    已结题

项目摘要

Project Summary/Abstract for, “Transplantation of MHC homozygous vascular progenitors in primates.” For tissue engineered arteries, the use of patient specific iPS cells would be severely limited by time constraints and cost. Banking iPS cells from rare individuals homozygous for HLA alleles has been proposed as a strategy to allow economies of scale, while still reducing rejection of iPS cell-derived transplanted tissues. Only a few hundred such cell lines would provide matches for the majority of the U.S. population, and the Waisman Clinical Biomanufacturing facility here on the University of Wisconsin has already produced cGMP HLA homozygous iPS cell lines. However, the immunological value of such an approach remains untested in an animal model with an immune system similar to the human immune system. Here we will use a unique population of MHC defined cynomolgus monkeys to test the immune response to MHC homozygous cynomolgus iPS cell-derived vascular cells transplanted to MHC haploidentical recipients. Using the MHC defined cynomolgus monkeys, we will use a limb ischemia model to determine the ability of iPS cell-derived arterial endothelial cells to contribute to collateral circulation when transplanted by themselves, in combination with iPS cell-derived smooth muscle cells, or when combined into a fully tissue engineered artery. A central premise of this proposal is that properly specified early arterial endothelial cells will robustly recruit, expand, and mature endogenous or co-transplanted smooth muscle cell progenitors to increase arteriogenesis in vivo, and that these arterial endothelial cells will be critical to producing tissue engineered arteries ex vivo that remain functional long after transplantation. The final goal of this proposal is to produce cGMP vascular progenitors from HLA homozygous human iPS cell lines for the pre-clinical animal studies required to file an IND for critical limb ischemia. With extensive human and primate pluripotent stem cell expertise, a strong bioengineering department, a National Primate Research Center with an MHC typing facility, and a GMP cell manufacturing facility, the environment at the University of Wisconsin is uniquely suited for completing the goals of this proposal.
“灵长类动物MHC纯合血管祖细胞移植”项目摘要/摘要

项目成果

期刊论文数量(0)
专著数量(0)
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Igor I. Slukvin其他文献

Assessment of safety and immunogenicity of MHC homozygous iPSC-derived CD34sup+/sup hematopoietic progenitors in an NHP model
  • DOI:
    10.1182/bloodadvances.2022006984
  • 发表时间:
    2022-09-27
  • 期刊:
  • 影响因子:
    7.100
  • 作者:
    Saritha S. D'Souza;Akhilesh Kumar;John Maufort;Jason T. Weinfurter;Matthew Raymond;Nick S. Strelchenko;Elizabeth Perrin;Jennifer Coonen;Andres Mejia;Heather A. Simmons;Bruce E. Torbett;Matthew Reynolds;James A. Thomson;Igor I. Slukvin
  • 通讯作者:
    Igor I. Slukvin
CELLULES ÉRYTHROÏDES PRODUISANT DE L'HÉMOGLOBINE ADULTE DE TYPE β GÉNÉRÉE À PARTIR DE CELLULES SOUCHES EMBRYONNAIRES
β 型成人血红蛋白生成细胞和胚胎细胞部分
  • DOI:
  • 发表时间:
    2007
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Igor I. Slukvin;James A. Thomson;M. Vodyanyk;Maryna E. Gumenyuk
  • 通讯作者:
    Maryna E. Gumenyuk
Methode d'elaboration de cellules dendritiques a partir de cellules souches embryonnaires
细胞树突和胚胎细胞部分的详细阐述方法
  • DOI:
  • 发表时间:
    2006
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Igor I. Slukvin;James A. Thomson;M. Vodyanyk;Maryna E. Gumenyuk
  • 通讯作者:
    Maryna E. Gumenyuk
Small-diameter artery grafts engineered from pluripotent stem cells maintain 100% patency in an allogeneic rhesus macaque model
由多能干细胞工程化的小直径动脉移植物在同种异体恒河猴模型中保持 100%的通畅率。
  • DOI:
    10.1016/j.xcrm.2025.102002
  • 发表时间:
    2025-03-18
  • 期刊:
  • 影响因子:
    10.600
  • 作者:
    Jue Zhang;Diana Marcela Tabima;David Vereide;Weifeng Zeng;Nicholas J. Albano;Sarah Lyon;Peter J. Nicksic;Ellen C. Shaffrey;Robert E. George;Mitchell D. Probasco;Elizabeth S. Perrin;Yiyang Xu;Matthew E. Brown;Ron Stewart;Naomi C. Chesler;Lih-Sheng Turng;Samuel O. Poore;Igor I. Slukvin;James A. Thomson;John P. Maufort
  • 通讯作者:
    John P. Maufort

Igor I. Slukvin的其他文献

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{{ truncateString('Igor I. Slukvin', 18)}}的其他基金

Molecular Determinants of Hemogenic Endothelium
造血内皮的分子决定因素
  • 批准号:
    10187643
  • 财政年份:
    2018
  • 资助金额:
    $ 115.49万
  • 项目类别:
Molecular Determinants of Hemogenic Endothelium
造血内皮的分子决定因素
  • 批准号:
    9975885
  • 财政年份:
    2018
  • 资助金额:
    $ 115.49万
  • 项目类别:
Nonhuman Primate Model for Preclinical Evaluation of Haplotype-Based iPSC Banking for HLA-matched Blood Products
用于 HLA 匹配血液制品基于单倍型 iPSC 库的临床前评估的非人灵长类动物模型
  • 批准号:
    9153287
  • 财政年份:
    2016
  • 资助金额:
    $ 115.49万
  • 项目类别:
Nonhuman Primate Model for Preclinical Evaluation of Haplotype-Based iPSC Banking for HLA-matched Blood Products
用于 HLA 匹配血液制品基于单倍型 iPSC 库的临床前评估的非人灵长类动物模型
  • 批准号:
    9276794
  • 财政年份:
    2016
  • 资助金额:
    $ 115.49万
  • 项目类别:
Transplantation of MHC Homozygous Vascular Progenitors in Primates
灵长类 MHC 纯合血管祖细胞移植
  • 批准号:
    10416029
  • 财政年份:
    2016
  • 资助金额:
    $ 115.49万
  • 项目类别:
iPSC-based blood regenerative therapies for AIDS
基于 iPSC 的艾滋病血液再生疗法
  • 批准号:
    9057122
  • 财政年份:
    2013
  • 资助金额:
    $ 115.49万
  • 项目类别:
iPSC-based blood regenerative therapies for AIDS
基于 iPSC 的艾滋病血液再生疗法
  • 批准号:
    8708198
  • 财政年份:
    2013
  • 资助金额:
    $ 115.49万
  • 项目类别:
iPSC-based blood regenerative therapies for AIDS
基于 iPSC 的艾滋病血液再生疗法
  • 批准号:
    8603133
  • 财政年份:
    2013
  • 资助金额:
    $ 115.49万
  • 项目类别:
iPSC-based blood regenerative therapies for AIDS
基于 iPSC 的艾滋病血液再生疗法
  • 批准号:
    9268021
  • 财政年份:
    2013
  • 资助金额:
    $ 115.49万
  • 项目类别:
ES Cell-Specific Genes and Reprogramming of Human Somatic Cells
ES细胞特异性基因和人类体细胞重编程
  • 批准号:
    8381277
  • 财政年份:
    2012
  • 资助金额:
    $ 115.49万
  • 项目类别:

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