Immunomodulatory Properties of BCG: Implications for MTCT
卡介苗的免疫调节特性:对 MTCT 的影响
基本信息
- 批准号:8294777
- 负责人:
- 金额:$ 13.05万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2008
- 资助国家:美国
- 起止时间:2008-06-05 至 2014-05-31
- 项目状态:已结题
- 来源:
- 关键词:AdultBiological AssayBirthBlood CellsBreast FeedingCCR5 geneCD4 Lymphocyte CountCXCR4 geneCellsChildChildhoodClinicalCollaborationsEvaluationExposure toFosteringFoundationsFutureGreen Fluorescent ProteinsHIVHIV InfectionsHIV SeropositivityHIV-1Human MilkImmuneImmune responseImmunityImmunologicsImmunologyIn VitroIncidenceInfantInfectionInternationalInvestigationLactationLatin AmericaLow incomeMeasuresMononuclearMothersNeonatalPatientsPerinatalPeripheral Blood Mononuclear CellPhasePostdoctoral FellowPredispositionProductionPropertyProphylactic treatmentRANTESRecombinantsResearchRiskSerumSmall Inducible Cytokine A3StigmataTestingTimeTuberculosis VaccinesUmbilical Cord BloodVaccinatedVaccinationViral Load resultWaterWomanantiretroviral therapybasechemokinechemokine receptorclinical effectcostfeedingimmune activationimmunogenicityin vivoneonatenovelpathogenreceptorreceptor expressionskillssocial stigmastemtransmission processvaccination against tuberculosisvaccination strategy
项目摘要
DESCRIPTION (provided by applicant): The purpose of this proposal is to test the hypothesis that neonatal BCG vaccination leads to activation of immune response that may increase the susceptibility to HIV infection in breast-fed infants of HIV-positive mothers. This investigation stems from the candidate's research as a post-doctoral fellow in heterologous ^ immunity and will allow her to develop expertise in patient-based immunology research. Furthermore, it provides a long-sought opportunity to develop skills in international research and foster an academic collaboration with Latin America, the candidate's region of origin. The specific aim of this proposal is to evaluate the effect of BCG on expression of co-receptors required for HIV entry (CCR5 and CXCR4) and on susceptibility of mononuclear cells to HIV infection. The initial phase of the study will be performed in vitro, using cord blood cells from healthy infants. Determination of co- receptors levels will be performed using FACS analysis and single-round HIV infection will be measured with a recombinant GFP-expressing HIV-1. The clinical phase of the study will proceed after optimization of all assays involved, and will involve healthy neonates. This phase is a longitudinal assessment of co-receptor expression and HIV susceptibility of mononuclear cells before and after BCG vaccination. Activation markers and HIV susceptibility will be measured in cord blood mononuclear cells (pre-BCG) and peripheral blood mononuclear cells obtained from the same infants 6 weeks'after BCG vaccination. A significant proportion of infants infected with HIV through maternal-to-child-transmission (MTCT) are infected in the post-partum period through exposure to breastmilk. Although exclusive replacement feeding eliminates the risk of HIV acquisition through lactation, this option is often not safe or feasible for women in lower income settings due to stigma, high cost of formula, or poor access to potable water. The results from this study will provide a conceptual immunologic basis for further exploring the clinical effect of BCG-induced immune activation on host susceptibility to HIV. The effect of BCG on co-receptors required for HIV entry may have implications regarding the timing of BCG vaccination in HIV-exposed breastfed neonates.
描述(由申请方提供):本提案的目的是检验以下假设:新生儿卡介苗接种导致免疫应答激活,可能增加HIV阳性母亲母乳喂养的婴儿对HIV感染的易感性。这项调查源于候选人作为异源免疫博士后研究员的研究,并将使她能够发展以患者为基础的免疫学研究的专业知识。此外,它提供了一个长期寻求的机会,以发展国际研究技能,并促进与拉丁美洲,候选人的原籍地区的学术合作。该提案的具体目的是评估BCG对HIV进入所需的辅助受体(CCR 5和CXCR 4)表达以及单核细胞对HIV感染的易感性的影响。研究的初始阶段将在体外进行,使用来自健康婴儿的脐带血细胞。将使用FACS分析进行共受体水平的测定,并使用表达重组GFP的HIV-1测量单轮HIV感染。本研究的临床阶段将在优化所有相关测定后进行,并将涉及健康新生儿。这一阶段是对卡介苗接种前后单核细胞的共受体表达和HIV易感性的纵向评估。将在BCG接种后6周从相同婴儿获得的脐带血单核细胞(pre-BCG)和外周血单核细胞中测量活化标志物和HIV易感性。在通过母婴传播感染艾滋病毒的婴儿中,很大一部分是在产后期间通过接触母乳而感染的。虽然完全替代喂养消除了通过哺乳感染艾滋病毒的风险,但由于耻辱、配方奶成本高或难以获得饮用水,这种选择对低收入环境中的妇女往往不安全或不可行。本研究结果将为进一步探讨BCG诱导的免疫激活对宿主对HIV易感性的临床作用提供概念性免疫学依据。BCG对HIV进入所需的辅助受体的作用可能对HIV暴露的母乳喂养的新生儿接种BCG疫苗的时机产生影响。
项目成果
期刊论文数量(8)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
A systematic review of HIV/AIDS survival and delayed diagnosis among Hispanics in the United States.
- DOI:10.1007/s10903-011-9497-y
- 发表时间:2012-02
- 期刊:
- 影响因子:1.9
- 作者:Chen, Nadine E.;Gallant, Joel E.;Page, Kathleen R.
- 通讯作者:Page, Kathleen R.
Mycobacterium tuberculosis complex enhances susceptibility of CD4 T cells to HIV through a TLR2-mediated pathway.
- DOI:10.1371/journal.pone.0041093
- 发表时间:2012
- 期刊:
- 影响因子:3.7
- 作者:Thayil SM;Ho YC;Bollinger RC;Blankson JN;Siliciano RF;Karakousis PC;Page KR
- 通讯作者:Page KR
TLR2-Modulating Lipoproteins of the Mycobacterium tuberculosis Complex Enhance the HIV Infectivity of CD4+ T Cells.
- DOI:10.1371/journal.pone.0147192
- 发表时间:2016
- 期刊:
- 影响因子:3.7
- 作者:Skerry C;Klinkenberg LG;Page KR;Karakousis PC
- 通讯作者:Karakousis PC
Faculty diversity programs in U.S. medical schools and characteristics associated with higher faculty diversity.
- DOI:10.1097/acm.0b013e31822c066d
- 发表时间:2011-10
- 期刊:
- 影响因子:0
- 作者:Page KR;Castillo-Page L;Wright SM
- 通讯作者:Wright SM
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Kathleen R Page其他文献
Ensuring the right to health for migrants and refugees
确保移民和难民的健康权
- DOI:
10.1016/s2352-3018(24)00275-3 - 发表时间:
2024-12-01 - 期刊:
- 影响因子:13.000
- 作者:
Andrea L Wirtz;Kathleen R Page;Paul B Spiegel - 通讯作者:
Paul B Spiegel
Kathleen R Page的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Kathleen R Page', 18)}}的其他基金
REDES: a peer network and mobile health (mHealth) enhanced CHW model to maximize COVID-19 vaccination among low income Latinos
REDES:对等网络和移动医疗 (mHealth) 增强的 CHW 模型,可最大限度地提高低收入拉丁美洲人的 COVID-19 疫苗接种率
- 批准号:
10419233 - 财政年份:2022
- 资助金额:
$ 13.05万 - 项目类别:
Mentoring in Community-engaged Research to Promote Health Equity for Latinos
指导社区参与的研究,以促进拉丁裔的健康公平
- 批准号:
10506457 - 财政年份:2022
- 资助金额:
$ 13.05万 - 项目类别:
REDES: a peer network and mobile health (mHealth) enhanced CHW model to maximize COVID-19 vaccination among low income Latinos
REDES:对等网络和移动医疗 (mHealth) 增强的 CHW 模型,可最大限度地提高低收入拉丁美洲人的 COVID-19 疫苗接种率
- 批准号:
10609761 - 财政年份:2022
- 资助金额:
$ 13.05万 - 项目类别:
Sin Duda: a community-driven approach to expand reach, access and uptake of COVID-19 home-based tests for at risk Latinos
Sin Duda:一种社区驱动的方法,旨在扩大针对高危拉丁美洲人的 COVID-19 家庭测试的覆盖范围、获取和采用率
- 批准号:
10445509 - 财政年份:2022
- 资助金额:
$ 13.05万 - 项目类别:
Mentoring in Community-engaged Research to Promote Health Equity for Latinos
指导社区参与的研究,以促进拉丁裔的健康公平
- 批准号:
10708867 - 财政年份:2022
- 资助金额:
$ 13.05万 - 项目类别:
Sin Duda: a community-driven approach to expand reach, access and uptake of COVID-19 home-based tests for at risk Latinos
Sin Duda:一种社区驱动的方法,旨在扩大针对高危拉丁美洲人的 COVID-19 家庭测试的覆盖范围、获取和采用率
- 批准号:
10544767 - 财政年份:2022
- 资助金额:
$ 13.05万 - 项目类别:
Improving the Diagnosis of Histoplasmosis in an Endemic Region
改善流行地区组织胞浆菌病的诊断
- 批准号:
8329749 - 财政年份:2011
- 资助金额:
$ 13.05万 - 项目类别:
Immunomodulatory Properties of BCG: Implications for MTCT
卡介苗的免疫调节特性:对 MTCT 的影响
- 批准号:
7419467 - 财政年份:2008
- 资助金额:
$ 13.05万 - 项目类别:
Immunomodulatory Properties of BCG: Implications for MTCT
卡介苗的免疫调节特性:对 MTCT 的影响
- 批准号:
8072020 - 财政年份:2008
- 资助金额:
$ 13.05万 - 项目类别:
Immunomodulatory Properties of BCG: Implications for MTCT
卡介苗的免疫调节特性:对 MTCT 的影响
- 批准号:
7631459 - 财政年份:2008
- 资助金额:
$ 13.05万 - 项目类别:
相似海外基金
Establishment of a new biological assay using Hydra nematocyst deployment
利用水螅刺丝囊部署建立新的生物测定方法
- 批准号:
520728-2017 - 财政年份:2017
- 资助金额:
$ 13.05万 - 项目类别:
University Undergraduate Student Research Awards
POINT-OF-CARE BIOLOGICAL ASSAY FOR DETERMINING TISSUE-SPECIFIC ABSORBED IONIZING RADIATION DOSE (BIODOSIMETER) AFTER RADIOLOGICAL AND NUCLEAR EVENTS.
用于确定放射和核事件后组织特异性吸收电离辐射剂量(生物剂量计)的护理点生物测定。
- 批准号:
10368760 - 财政年份:2017
- 资助金额:
$ 13.05万 - 项目类别:
POINT-OF-CARE BIOLOGICAL ASSAY FOR DETERMINING TISSUE-SPECIFIC ABSORBED IONIZING RADIATION DOSE (BIODOSIMETER) AFTER RADIOLOGICAL AND NUCLEAR EVENTS.
用于确定放射和核事件后组织特异性吸收电离辐射剂量(生物剂量计)的护理点生物测定。
- 批准号:
10669539 - 财政年份:2017
- 资助金额:
$ 13.05万 - 项目类别:
POINT-OF-CARE BIOLOGICAL ASSAY FOR DETERMINING TISSUE-SPECIFIC ABSORBED IONIZING RADIATION DOSE (BIODOSIMETER) AFTER RADIOLOGICAL AND NUCLEAR EVENTS.
用于确定放射和核事件后组织特异性吸收电离辐射剂量(生物剂量计)的护理点生物测定。
- 批准号:
9570142 - 财政年份:2017
- 资助金额:
$ 13.05万 - 项目类别:
POINT-OF-CARE BIOLOGICAL ASSAY FOR DETERMINING TISSUE-SPECIFIC ABSORBED IONIZING RADIATION DOSE (BIODOSIMETER) AFTER RADIOLOGICAL AND NUCLEAR EVENTS.
用于确定放射和核事件后组织特异性吸收电离辐射剂量(生物剂量计)的护理点生物测定。
- 批准号:
9915803 - 财政年份:2017
- 资助金额:
$ 13.05万 - 项目类别:
COVID-19 Supplemental work: POINT-OF-CARE BIOLOGICAL ASSAY FOR DETERMINING TISSUE-SPECIFIC ABSORBED IONIZING RADIATION DOSE (BIODOSIMETER).
COVID-19 补充工作:用于确定组织特异性吸收电离辐射剂量的护理点生物测定(生物剂量计)。
- 批准号:
10259999 - 财政年份:2017
- 资助金额:
$ 13.05万 - 项目类别:
Drug discovery based on a new biological assay system using Yeast knock-out strain collection
基于使用酵母敲除菌株收集的新生物测定系统的药物发现
- 批准号:
21580130 - 财政年份:2009
- 资助金额:
$ 13.05万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Machine learning for automatic gene annotation using high-throughput biological assay data
使用高通量生物测定数据进行自动基因注释的机器学习
- 批准号:
300985-2004 - 财政年份:2005
- 资助金额:
$ 13.05万 - 项目类别:
Postdoctoral Fellowships
Machine learning for automatic gene annotation using high-throughput biological assay data
使用高通量生物测定数据进行自动基因注释的机器学习
- 批准号:
300985-2004 - 财政年份:2004
- 资助金额:
$ 13.05万 - 项目类别:
Postdoctoral Fellowships