X-Ray Chrystallography

X射线结晶学共享资源 X射线晶体学共享资源为KCC调查人员提供了获得结构 与他们的研究兴趣直接相关的信息。这包括确定从头开始的结构， 点突变、与包括抑制剂在内的小分子共结晶的结构后果 潜在的治疗方法，以及包括蛋白质、DNA和/或RNA在内的多组分系统。虽然结构 决心是一项高度专业化、劳动密集型的工作，从这些工作中获得的信息 调查经常涉及看似不同的生化数据，并总是改善未来 研究努力。 在改善KCC社区使用该设施方面取得了重大进展。例如，一个 液体搬运机器人现在可以用更少的材料和人力测试结晶的可行性， 使初步筛选试验具有成本效益。这鼓励了KCC的一些成员 考虑在他们的项目中使用结构生物学。生物化学和分子生物学系 认识到结构生物学对大学的重要性，并增聘了结构生物学 助理教授级别的生物学。随着用户的增加和新员工的增加，我们预计 一些新的结构即将问世，其中大多数与癌症的分子起源直接相关。

X-RAY CRYSTALLOGRAPHY SHARED RESOURCE The X-ray Crystallography shared resource provides KCC investigators the ability to obtain structural information directly relevant to their research interests. This includes the determination of de novo structures, structural consequences of point mutations, co-crystallization with small molecules including inhibitors and potential therapeutics, and multicomponent systems including proteins, DNA and/or RNA. Although structure determination is a highly specialized, labor-intensive endeavor, the information obtained from these investigations frequently addresses seemingly disparate biochemical data and invariably improves future research efforts. Significant progress has been made to improve access to the facility for the KCC community. For instance, a liquid handling robot now tests for crystallization feasibility with significantly less material and personal effort, making preliminary screening experiments cost effective. This has encouraged a number of KCC members to consider employing structural biology in their program. The Department of Biochemistry and Molecular Biology recognizes the significance of structural biology to the university, and has made an additional hire in structural biology at the assistant professor level. With the increase of users and the new hire, we anticipate that a number of new structures will be forthcoming, most with immediate relevance to molecular origin of cancer.