ANIMAL RESOURCES

动物资源

• 批准号: 8378389
• 负责人: William B. Stallcup
• 金额: $ 21.29万
• 依托单位: SANFORD BURNHAM PREBYS MEDICAL DISCOVERY INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-05-21 至 2015-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8378389
• 关键词: Accreditation; Anesthesia procedures; Animal Experimentation; Animal Model; Animal Welfare; Animals; Back; Blood; Breeding; Budgets; Cancer Center; Cancer Center Support Grant; Cancer Etiology; Capital; Caring; Charge; Chemical Agents; Chemicals; Collection; Development; Ear; Embryo; Ensure; Equipment; Funding; Future; Genetic; Genomics; Hematology; Housing; Human Resources; Image; Image Analysis; Immune; Injection of therapeutic agent; Institutes; Knock-out; Knockout Mice; Label; Luciferases; Maintenance; Malignant Neoplasms; Measurement; Mission; Molecular; Mus; Neoplasm Metastasis; Operative Surgical Procedures; Participant; Pharmaceutical Preparations; Phenotype; Procedures; Publications; Research Activity; Research Personnel; Resources; Sampling; Sanitation; Serum; Services; Stromal Neoplasm; Study models; Tail; Testing; Therapeutic; Tissue Banks; Training; Transgenic Mice; Transgenic Mouse Facility; Transgenic Organisms; Tumor Angiogenesis; Tumor Cell Line; Ultrasonography; Visual; Weaning; animal care; animal facility; animal resource; base; cost; experience; in vivo; instrumentation; intravital microscopy; member; mouse model; notch protein; programs; research facility; research study; square foot; standard of care; success; trafficking; tumor; tumor growth; tumor progression

Animal models are essential for understanding the molecular causes for cancers and assessing potential therapeutic approaches. The facility's mission is to maximize the scientific benefits of animal experimentation, while maintaining an emphasis on animal welfare. Both of these efforts have been successful. From the animal welfare standpoint. Animal Resources has been AAALAC accredited since 1988, with the most recent renewal completed in March 2009. Scientifically, the facility has been critical to the success of the Cancer Center, supporting a wide variety of studies, which resulted in over 200 publications in the past funding period. Animal Resources occupies 12,800 sq. ft. of space, almost 10,000 of which belong to the centralized breeding facility, which has been expanded and upgraded to all ventilated caging. For active experimentation, mice are transferred to a facility in the building next door (1,740 sq. ft.). The Resource currently operates at 76% of its current capacity of 9,400 cages, housing more than 480 genetically altered lines of mice. Services include: 1] husbandry 2] breeding colony maintenance (the $0.64/cage per diem rate includes husbandry, breeding, weaning, ear notching, tail sampling) 3] transgenic/knockout mouse facility, 4] in vivo imaging and analysis facility, and 5] technical support (e.g., blood collection, injections, drug administration, tumor measurements, anesthesia, surgeries, post-op care). Operating costs of Resource services are recovered largely on a charge-back basis from facility users, with capital equipment, equipment maintenance, and construction funds provided from Institute support. The current funding period has seen an increase in the use of vivarium resources to study cancer-related phenomena. The Facility offers several standardized models for studying tumor formation and metastasis, and will continue to expand this effort. The In Vivo imaging and Analysis Facility, initiated in the last funding period, supports bioluminescent and fluorescent imaging (Xenogen IVIS200), ultrasound imaging (Visual Sonics Vevo770), along with hematology and serum analysis. The Resource stocks a panel of well characterized standard and luciferase-labeled tumor cell lines, to expand investigators' ability to quantify and characterize tumor progression. Additional imaging instrumentation, such as intravital microscopy and microPET will be evaluated for future acquisition. In 2008, 96% of the Resource users were Cancer Center members, with a total of 40 Cancer Center investigators from all four Programs utilizing the Resource. $124,455 in CCSG support is requested in the first year, representing 4.6% of the total estimated annual operating budget.

动物模型对于了解癌症的分子原因和评估潜力至关重要 治疗方法。该设施的使命是最大限度地提高动物的科学效益 实验，同时保持对动物福利的重视。这两项努力都已 成功的。从动物福利的角度来看。动物资源已获得 AAALAC 认证 1988 年，最近一次更新于 2009 年 3 月完成。从科学角度来说，该设施对于 癌症中心的成功支持了各种各样的研究，这些研究产生了 200 多项 过去资助期间的出版物。动物资源中心占地 12,800 平方英尺，近 10,000 平方英尺。 属于集中养殖设施，现已扩建升级为全通风 笼养。为了进行主动实验，小鼠被转移到隔壁大楼（1,740 平方英尺）的设施中。 该资源目前已运营 9,400 个笼子，可容纳 480 多个笼子，目前利用率为 76% 基因改变的小鼠品系。 服务包括： 1] 饲养 2] 繁殖群体维护（每日 0.64 美元/笼的费率包括 畜牧业、繁殖、断奶、耳部切口、尾部取样）3] 转基因/基因敲除小鼠设施，4] 体内 成像和分析设施，以及 5] 技术支持（例如采血、注射、给药、 肿瘤测量、麻醉、手术、术后护理）。资源服务的运营成本为 大部分在退款的基础上从设施用户处收回，包括资本设备、设备 维护和建设资金由研究所提供支持。目前资助期限已见 增加使用动物园资源来研究癌症相关现象。该设施提供多种 研究肿瘤形成和转移的标准化模型，并将继续扩大这一努力。 体内成像和分析设施于上一个资助期启动，支持生物发光和 荧光成像 (Xenogen IVIS200)、超声成像 (Visual Sonics Vevo770) 以及 血液学和血清分析。该资源库存有一组特征明确的标准和 荧光素酶标记的肿瘤细胞系，以扩大研究人员量化和表征肿瘤的能力 进展。其他成像仪器，例如活体显微镜和 microPET 评估未来的收购。 2008 年，96% 的资源用户是癌症中心会员，其中 来自所有四个项目的总共 40 名癌症中心研究人员使用该资源。 CCSG 124,455 美元 第一年请求的支持占预计年度运营预算总额的 4.6%。