CORE--Shared Resources Animal Facility

描述（由申请人提供）：动物资源机构的使命是使 伯纳姆研究所癌症中心的研究人员利用小动物进行生物医学研究，这一使命一直是并将继续是癌症中心成功的关键。该机构作为一个集中服务机构运作，其中大多数程序由经过培训的机构工作人员执行。这确保了一致的护理标准，最大限度地减少了通过设施的交通，并使癌症中心的研究人员不必雇用和培训自己的人员来完成这些任务。该设施提供三种类型的服务：小鼠遗传学，饲养和体内分析。Mouse Genetics创建新的转基因和基因敲除小鼠品系，Husbandry维护和管理现有的动物群体，In Vivo Analysis帮助描述用于实验研究的动物的表型。由体内分析服务支持的当前和计划中的癌症相关项目包括使用异种移植和转基因小鼠模型来研究肿瘤诱导和进展的机制，沿着测试新药减缓肿瘤生长和转移的功效。是 设想该中心的药物发现计划将导致新化合物的鉴定，这些化合物将需要在体内测试对肿瘤生长和转移的活性。为了帮助评估这些药物，体内分析服务将提供集中的肿瘤分析核心，以建立和评估转基因和异种移植模型中的肿瘤发病和进展。该核心的具体目标是：1）提供有关动物模型使用、药物制剂和药物给药的咨询和信息; 2）维持两个转基因小鼠群体TRAMP和MMTV-PyMT，用于前列腺癌和乳腺癌的相应研究。这些细胞系中的从头肿瘤发生密切模拟在人类病例中所见的; 3）维持裸鼠和MDA-MB-435乳腺癌和PC-3前列腺癌细胞系的原种，分别用于针对人类乳腺和前列腺肿瘤的药物有效性的异种移植研究; 4）启动肿瘤生长（通过繁殖或异种移植）， 量化对照和治疗动物中的肿瘤发病、生长和转移; 5）与信息学和数据管理资源合作，以维护数据档案，用于不同药物试验的比较。

DESCRIPTION (provided by applicant): The mission of the Animal Resources Facility is to enable Burnham Institute Cancer Center investigators to use small animals for biomedical research, a mission that has been and will continue to be critical to the success of the Cancer Center. The Facility operates as a centralized service in which most procedures are performed by trained Facility staff. This ensures a consistent standard of care, minimizes traffic through the Facility, and frees Cancer Center investigators from having to hire and train their own personnel for these tasks. The Facility provides three types of services: Mouse Genetics, Husbandry, and In Vivo Analysis. Mouse Genetics creates new lines of transgenic and gene knockout mice, Husbandry maintains and manages existing colonies of animals, and In Vivo Analysis helps to characterize the phenotypes of animals used for experimental studies. Current and projected cancer-related projects supported by the In Vivo Analysis service include the use of xenograft and transgenic mouse models to study mechanisms of tumor induction and progression, along with testing the efficacy of new agents for slowing tumor growth and metastasis. It is envisioned that the Center's Drug Discovery Initiative will lead to the identification of new compounds that will need to be tested in vivo for activity against tumor growth and metastasis. To aid in the evaluation of these drugs, the In Vivo Analysis service will offer a centralized Tumor Analysis Core to establish and evaluate tumor onset and progression in transgenic and xenograft models. Specific Aims for this core will be to: 1) Offer consultation and information about the use of animal models, drug formulation, and drug administration; 2) Maintain two transgenic mouse colonies, TRAMP and MMTV-PyMT, for the respective study of prostate and mammary cancer. De novo tumorigenesis in these lines closely mimics that seen in human cases; 3) Maintain nude mice and stocks of MDA-MB-435 breast cancer and PC-3 prostate cancer cell lines for xenograft studies of drug effectiveness against human mammary and prostate tumors, respectively; 4) Initiate tumor growth (by breeding or xenografting) and quantify tumor onset, growth, and metastasis in control and treated animals and ; 5) Collaborate with Informatics and Data Management Resources to maintain data archives for comparison of different drug trials.