CORE--Shared Resources Animal Facility

描述(由申请人提供)：动物资源基金的使命是使 伯纳姆研究所癌症中心的研究人员利用小动物进行生物医学研究，这项任务一直是，并将继续是癌症中心成功的关键。该设施作为一个中央服务机构运作，其中大多数程序由训练有素的设施工作人员执行。这确保了一致的护理标准，最大限度地减少了通过该设施的流量，并使癌症中心的调查人员不必为这些任务雇用和培训自己的人员。该机构提供三种类型的服务：小鼠遗传学、畜牧学和活体分析。小鼠遗传学创造了转基因和基因敲除小鼠的新品系，畜牧业维护和管理现有的动物群体，在活体分析中帮助确定用于实验研究的动物的表型。目前和计划中的癌症相关项目由体内分析服务支持，包括使用异种移植和转基因小鼠模型来研究肿瘤诱导和进展的机制，以及测试减缓肿瘤生长和转移的新药物的有效性。它是 设想该中心的药物发现倡议将导致识别新的化合物，这些化合物将需要在体内测试抗肿瘤生长和转移的活性。为了帮助评估这些药物，体内分析服务将提供一个集中的肿瘤分析核心，以建立和评估转基因和异种移植模型中的肿瘤发生和发展。这一核心的具体目标将是：1)提供关于动物模型、药物配方和给药的使用的咨询和信息；2)维持两个转基因小鼠群体，TRAMP和MMTV-PYMT，分别用于前列腺癌和乳腺癌的研究。这些细胞系中的新生肿瘤发生与在人类病例中看到的非常相似；3)保持裸鼠和储存的MDA-MB-435乳腺癌和PC-3前列腺癌细胞系，分别用于抗人类乳腺和前列腺癌的药物有效性的异种移植研究；4)启动肿瘤生长(通过繁殖或异种移植)和 量化对照和治疗动物的肿瘤发病、生长和转移；5)与信息学和数据管理资源合作，维护数据档案，用于不同药物试验的比较。

DESCRIPTION (provided by applicant): The mission of the Animal Resources Facility is to enable Burnham Institute Cancer Center investigators to use small animals for biomedical research, a mission that has been and will continue to be critical to the success of the Cancer Center. The Facility operates as a centralized service in which most procedures are performed by trained Facility staff. This ensures a consistent standard of care, minimizes traffic through the Facility, and frees Cancer Center investigators from having to hire and train their own personnel for these tasks. The Facility provides three types of services: Mouse Genetics, Husbandry, and In Vivo Analysis. Mouse Genetics creates new lines of transgenic and gene knockout mice, Husbandry maintains and manages existing colonies of animals, and In Vivo Analysis helps to characterize the phenotypes of animals used for experimental studies. Current and projected cancer-related projects supported by the In Vivo Analysis service include the use of xenograft and transgenic mouse models to study mechanisms of tumor induction and progression, along with testing the efficacy of new agents for slowing tumor growth and metastasis. It is envisioned that the Center's Drug Discovery Initiative will lead to the identification of new compounds that will need to be tested in vivo for activity against tumor growth and metastasis. To aid in the evaluation of these drugs, the In Vivo Analysis service will offer a centralized Tumor Analysis Core to establish and evaluate tumor onset and progression in transgenic and xenograft models. Specific Aims for this core will be to: 1) Offer consultation and information about the use of animal models, drug formulation, and drug administration; 2) Maintain two transgenic mouse colonies, TRAMP and MMTV-PyMT, for the respective study of prostate and mammary cancer. De novo tumorigenesis in these lines closely mimics that seen in human cases; 3) Maintain nude mice and stocks of MDA-MB-435 breast cancer and PC-3 prostate cancer cell lines for xenograft studies of drug effectiveness against human mammary and prostate tumors, respectively; 4) Initiate tumor growth (by breeding or xenografting) and quantify tumor onset, growth, and metastasis in control and treated animals and ; 5) Collaborate with Informatics and Data Management Resources to maintain data archives for comparison of different drug trials.