Molecular Diagnostic Core

分子诊断核心

• 批准号: 8293258
• 负责人: PETER A. ZIMMERMAN
• 金额: $ 14.57万
• 依托单位: CASE WESTERN RESERVE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-01 至 2017-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8293258
• 关键词: Address; Advanced Development; Alleles; American; Anopheles Genus; Anticoagulants; Antigens; Archives; Artemisinins; Asia; Biocompatible Materials; Biological; Biological Assay; Biostatistics Core; Blood; Blood specimen; Cataloging; Catalogs; Cellular biology; Clinical; Collaborations; Combined Modality Therapy; Complex; Cryopreservation; Culicidae; DNA; DNA Sequence; Data; Data Analyses; Databases; Dengue Virus; Detection; Development; Diagnosis; Diagnostic; Diagnostic Specificity; Ecology; Edetic Acid; Employee; Ensure; Entomology; Enzyme-Linked Immunosorbent Assay; Epidemiology; Erythrocytes; Evaluation; Family member; FarGo; Filaria bancrofti; Genetic; Genetic Markers; Genetic Polymorphism; Genome; Genotype; Health; Human; Human Parvovirus B19; Hygiene; Immunology; Individual; Infection; Insecticides; Institutes; Institution; Instruction; Intervention; Journals; Laboratories; Lead; Length; Leptospira interrogans; Leptospirosis; Leukocytes; Ligase; Location; Malaria; Measures; Medical Research; Methodology; Methods; Microsatellite Repeats; Microscopy; Microspheres; Molecular; Molecular Diagnostic Techniques; Monitor; Nucleic Acids; Papua New Guinea; Parasites; Participant; Pathogenesis; Performance; Permethrin; Pharmaceutical Preparations; Plasma; Plasmodium; Plasmodium falciparum; Polymorphic Microsatellite Marker; Population; Population Genetics; Population Sizes; Preparation; Procedures; Process; Public Health; Publishing; Queensland; RNA; Reaction; Research; Research Design; Research Personnel; Research Project Grants; Research Proposals; Resistance; Resources; Role; Sampling; Schedule; Series; Serum; Siblings; Single Nucleotide Polymorphism; Site; Solomon Islands; Source; Speed; Sporozoites; Staging; Structure; Students; Techniques; Technology; Technology Transfer; Thick; Training; Tropical Medicine; Tube; Universities; Update; Vanuatu; Vector-transmitted infectious disease; Whole Blood; Work; artemisinine; assay development; base; computerized data processing; cost; cost effective; disorder control; epidemiology study; experience; genome sequencing; human study; improved; member; nucleic acid purification; pathogen; programs; research study; sample collection; transmission process; vector; vector mosquito

PROJECT SUMMARY (See instructions): The Molecular Diagnostics Core will support the three major research projects and interact directly with the Database and Biostatistics Core in the organization of an archive of biological material that will be collected during the course ofthe research projects. Core activities include the application of existing methods for identifying parasite and mosquito species and strains by conventional microscopy, current molecular diagnostic methods, and by newly emerging cell biology and DNA sequencing technology. The Specific Aims are to: 1: Create an ICEMR Archive of human blood samples and mosquitoes for characterizing malaria species and strains, and for evaluating immunological parameters of infection by Plasmodium species and clinical malaria 2: Develop PCR-based assays to diagnose Plasmodium species Infections of human study participants and mosquitoes 3: Develop PCR-based assays to diagnose Infection by Plasmodium strains in human study participants and mosquitoes 4: In collaboration with the Transmission Project, develop PCR-based assays to perform mosquito speciation, process mosquitoes for blood meal source and monitor permethrin resistance 5: Develop microscopy- and PCR-based assays to diagnose Plasmodium gametocyte stages in human study participants 6: Evaluate and develop strategies based on advanced DNA sequencing strategies to analyze complexity of Plasmodium species infections and Anopheles mosquito diversity using polymorphisms distributed across the parasite and vector species genomes 7: Build capacity and transfer technology to partner institution laboratories in malaria endemic sites The Molecular Diagnostics Core will be directed by Prof. Zimmerman who has worked with PNGIMR (Mueller), the Swiss Tropical Institute (Felger) and UQ (Cooper and Beebe) on malaria epidemiology and mosquito ecology for >10 years. He will be assisted by I Felger, now at STI and previously an employee of PNGIMR. Capacity building and training in the malaria endemic site will be assured by the continuing high commitment of this team to expanding cost effective technologies to the region covered by the ICEMR.

项目摘要（请参阅说明）：分子诊断核心将支持三项主要研究 项目并直接与数据库和生物统计学核心进行互动 在研究项目过程中将收集的生物材料。核心活动包括 现有方法用于识别寄生虫和蚊子物种以及通过常规菌株的应用 显微镜，电流分子诊断方法以及新出现的细胞生物学和DNA测序 技术。具体目的是： 1：创建人类血液样本和蚊子的ICEMR档案，以表征疟疾物种 和菌株，并用于评估疟原虫和临床感染的免疫学参数 疟疾 2：开发基于PCR的测定法以诊断人类研究参与者的疟原虫感染和 蚊子 3：开发基于PCR的测定法，以诊断人类研究参与者的疟原虫菌株和 蚊子 4：与传输项目合作，开发基于PCR的测定法以执行蚊子 物种形成，血液粉源的过程蚊子和监测氯菊酯耐药性 5：开发基于显微镜和基于PCR的测定法以诊断人类的疟原虫阶段 研究参与者 6：根据先进的DNA测序策略评估和制定策略，以分析 疟原虫感染和蚊子蚊子多样性分布在 寄生虫和载体物种基因组 7：建立能力和转移技术向疟疾特有地点的合作伙伴机构实验室 分子诊断核心将由Zimmerman教授指导，他曾与PNGIMR合作 （Mueller），瑞士热带研究所（Felger）和UQ（Cooper and Beebe）关于疟疾流行病学和 蚊子生态学> 10年。他将得到I Felger的协助，现在在STI，以前是 pngimr。疟疾特有现场的能力建设和培训将由持续高 该团队致力于将ICEMR涵盖的地区扩展成本效益的技术扩展。