Multi-purpose Superresolution Microscope for Core Facility

用于核心设施的多用途超分辨率显微镜

• 批准号: 8246831
• 负责人: GOPAL THINAKARAN
• 金额: $ 51.06万
• 依托单位: UNIVERSITY OF CHICAGO
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-06-15 至 2013-06-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8246831
• 关键词: Biological Sciences; Books; Cells; Chicago; Confocal Microscopy; Core Facility; Electron Microscope; Funding; Growth; Hybrids; Institution; Lasers; Life; Lighting; Microscope; Microscopy; Noise; Optics; Research Personnel; Resolution; Resources; Scheme; Spottings; Structure; System; Technology; Time; United States National Institutes of Health; Universities; detector; improved; instrument; lens; light microscopy; novel; physical science; tool

DESCRIPTION (provided by applicant): Microscopes have improved over the years to permit ever greater sensitivity and precision, yet the focal spot diffraction limit of optical lenses has ot been broken until very recently. Stimulated Emission Depletion (STED) technology is a physical structuring of the illumination focus to produce effective illumination points 3-4 times smaller than the diffraction limit. Just as confocal microscopy provides 2-3 times the resolution of standard microscopy, STED "superresolution" outperforms confocal microscopy by at least this margin and closer approaches the resolution of the electron microscope. The Integrated Light Microscopy Facility (ILMF) at The University of Chicago serves more than 160 NIH-funded researchers in the Biological Sciences Division, as well as interdisciplinary researchers from the Physical Sciences Division and neighboring Institutions. During the past decade the ILMF has witnessed an explosive growth in the use of confocal microscopy. Particularly popular is the most advanced instrument in the ILMF: a Leica SP5 multiphoton confocal microscope, which is typically booked to capacity and must be reserved far in advance. The Leica SP5 II STED-CW microscope requested in this proposal is an enhanced version of the SP5 that includes greater sensitivity, and, most significantly, provides real-time superresolution capability. The instrument includes the (newer) continuous-wave depletion laser scheme that is better suited for live-cell probes. The system includes the latest technology, a hybrid detector that offers APD-like sensitivity with lower noise and wider dynamic range. The researchers at the University of Chicago require the resolution enhancement as well as the increased capacity, and have undertaken a lease to obtain these tools while replacing a failing, older SP2 confocal microscope in a Core serving 162 PIs and over 750 users, an action that demonstrates strong Institutional Commitment for this proposal. The seamlessly integrated STED capability is a major technical advance for researchers at The University of Chicago, and is a novel regional resource also available to researchers from other Institutions. PUBLIC HEALTH RELEVANCE: This request is to buyout an interim lease that the University of Chicago has undertaken to provide continued basic and newly-advanced high-end research microscopy, replacing a failing older system. The requested superresolution microscope has undergone extensive in-house testing, is housed in an established Core Facility, and provides novel capabilities that will significantly enhance the research programs of a large NIHfunded user community.

描述(申请人提供)：显微镜多年来一直在改进，以实现更高的灵敏度和精确度，但光学透镜的焦点衍射限制直到最近才被打破。受激发射耗尽(STED)技术是对照明焦点进行物理构造，以产生比衍射极限小3-4倍的有效照明点。就像共焦显微镜提供的分辨率是标准显微镜的2-3倍一样，STED的“超分辨率”至少比共焦显微镜高出这个界限，更接近电子显微镜的分辨率。芝加哥大学的集成光学显微镜设备(ILMF)为NIH资助的生物科学部160多名研究人员以及来自物理科学部和邻近机构的跨学科研究人员提供服务。在过去的十年中，ILMF见证了共焦显微镜使用的爆炸性增长。尤其受欢迎的是ILMF中最先进的仪器：徕卡SP5多光子共焦显微镜，它通常会被预订满，必须提前很长时间预订。本提案中要求的徕卡SP5 II STED-CW显微镜是SP5的增强版，包括更高的灵敏度，最重要的是，提供实时超分辨率能力。这台仪器 包括更适合活细胞探针的(较新的)连续波耗尽激光方案。该系统包括最新技术，一种混合探测器，它提供类似雪崩的灵敏度、更低的噪声和更宽的动态范围。芝加哥大学的研究人员要求提高分辨率以及增加容量，并承诺租用这些工具，同时在服务于162个PI和750多个用户的核心中更换故障的旧SP2共焦显微镜，这一行动表明了机构对这一提议的坚定承诺。无缝集成的STED能力是芝加哥大学研究人员的一项重大技术进步，也是一种新的地区性资源，也可供其他机构的研究人员使用。 与公共健康相关：这项请求是为了买断芝加哥大学承诺提供持续的基础和最新先进的高端研究显微镜的临时租约，以取代失败的旧系统。所要求的超分辨率显微镜经过了广泛的内部测试，安装在已建立的核心设施中，并提供了新的功能，将显著增强由NIH资助的大型用户社区的研究计划。