Multi-purpose Superresolution Microscope for Core Facility

用于核心设施的多用途超分辨率显微镜

• 批准号: 8246831
• 负责人: GOPAL THINAKARAN
• 金额: $ 51.06万
• 依托单位: UNIVERSITY OF CHICAGO
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-06-15 至 2013-06-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8246831
• 关键词: Biological Sciences; Books; Cells; Chicago; Confocal Microscopy; Core Facility; Electron Microscope; Funding; Growth; Hybrids; Institution; Lasers; Life; Lighting; Microscope; Microscopy; Noise; Optics; Research Personnel; Resolution; Resources; Scheme; Spottings; Structure; System; Technology; Time; United States National Institutes of Health; Universities; detector; improved; instrument; lens; light microscopy; novel; physical science; tool

DESCRIPTION (provided by applicant): Microscopes have improved over the years to permit ever greater sensitivity and precision, yet the focal spot diffraction limit of optical lenses has ot been broken until very recently. Stimulated Emission Depletion (STED) technology is a physical structuring of the illumination focus to produce effective illumination points 3-4 times smaller than the diffraction limit. Just as confocal microscopy provides 2-3 times the resolution of standard microscopy, STED "superresolution" outperforms confocal microscopy by at least this margin and closer approaches the resolution of the electron microscope. The Integrated Light Microscopy Facility (ILMF) at The University of Chicago serves more than 160 NIH-funded researchers in the Biological Sciences Division, as well as interdisciplinary researchers from the Physical Sciences Division and neighboring Institutions. During the past decade the ILMF has witnessed an explosive growth in the use of confocal microscopy. Particularly popular is the most advanced instrument in the ILMF: a Leica SP5 multiphoton confocal microscope, which is typically booked to capacity and must be reserved far in advance. The Leica SP5 II STED-CW microscope requested in this proposal is an enhanced version of the SP5 that includes greater sensitivity, and, most significantly, provides real-time superresolution capability. The instrument includes the (newer) continuous-wave depletion laser scheme that is better suited for live-cell probes. The system includes the latest technology, a hybrid detector that offers APD-like sensitivity with lower noise and wider dynamic range. The researchers at the University of Chicago require the resolution enhancement as well as the increased capacity, and have undertaken a lease to obtain these tools while replacing a failing, older SP2 confocal microscope in a Core serving 162 PIs and over 750 users, an action that demonstrates strong Institutional Commitment for this proposal. The seamlessly integrated STED capability is a major technical advance for researchers at The University of Chicago, and is a novel regional resource also available to researchers from other Institutions. PUBLIC HEALTH RELEVANCE: This request is to buyout an interim lease that the University of Chicago has undertaken to provide continued basic and newly-advanced high-end research microscopy, replacing a failing older system. The requested superresolution microscope has undergone extensive in-house testing, is housed in an established Core Facility, and provides novel capabilities that will significantly enhance the research programs of a large NIHfunded user community.

描述（由申请人提供）：显微镜多年来已经改进，以允许更高的灵敏度和精度，但光学透镜的焦点衍射极限直到最近才被突破。受激发射损耗（STED）技术是照明焦点的物理结构，以产生比衍射极限小3-4倍的有效照明点。正如共焦显微镜提供2-3倍的标准显微镜的分辨率，STED“超分辨率”优于共焦显微镜至少这个利润率和更接近的分辨率的电子显微镜。 芝加哥大学的集成光学显微镜设施（ILMF）为生物科学部的160多名NIH资助的研究人员以及物理科学部和邻近机构的跨学科研究人员提供服务。在过去的十年中，ILMF见证了共聚焦显微镜的爆炸性增长。特别受欢迎的是ILMF中最先进的仪器：Leica SP 5多光子共聚焦显微镜，通常被预订到容量，必须提前预订。本提案中要求的Leica SP 5 II STED-CW显微镜是SP 5的增强版，具有更高的灵敏度，最重要的是，提供实时超分辨率能力。仪器 包括（更新的）连续波损耗激光方案，其更适合于活细胞探针。该系统采用了最新技术，即混合探测器，可提供类似APD的灵敏度，同时具有更低的噪声和更宽的动态范围。芝加哥大学的研究人员需要提高分辨率和增加容量，并已承诺租赁这些工具，同时更换为162名PI和750多名用户服务的核心中的故障，较旧的SP2共聚焦显微镜，这一行动表明了对该提案的强烈机构承诺。无缝集成的STED能力是芝加哥大学研究人员的一项重大技术进步，也是其他机构研究人员可用的一种新的区域资源。 公共卫生相关性：这一请求是买断芝加哥大学已承诺提供持续的基本和新先进的高端研究显微镜的临时租赁，取代失败的旧系统。所要求的超分辨率显微镜已经过广泛的内部测试，被安置在一个既定的核心设施，并提供新的功能，将显着提高一个大型NIH资助的用户社区的研究计划。