Acquisition of a 500 MHz NMR System for Metabolic Studies

获取用于代谢研究的 500 MHz NMR 系统

• 批准号: 8245367
• 负责人: ROBIN A DE GRAAF
• 金额: $ 50万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-05-08 至 2013-11-07
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8245367
• 关键词: Biochemical; Blood; Brain; Detection; Development; Funding; Future; Grant; Human; Label; Liquid substance; Magnetic Resonance; Measurement; Measures; Productivity; Research; Resolution; Rodent; Sampling; Support System; System; Time; United States National Institutes of Health; in vivo; innovation; instrument; medical schools; metabolic abnormality assessment; novel; programs

DESCRIPTION (provided by applicant): Through the NIH Shared Instrument Grant program funds are requested for a replacement of a 25-year old Bruker 500 MHz magnet and console with a new Agilent 500 MHz magnet and console. The current high- resolution, liquid-state NMR system is located in the basement of the Yale Magnetic Resonance Research Center, which is part of the premiere research building, The Anlyan Center, of the Yale School of Medicine. Frequent and repeated hardware failings, as well as stability and homogeneity issues of the old hardware makes a near future replacement mandatory in order to guarantee continued productivity on a large number of NIH-funded grants. The current 500 MHz system is the workhorse for a wide range of biochemical studies closely related to in vivo MR projects. These include, but are not limited to, the measurement of blood substrate levels and 13C fractional enrichments, the development of novel isotopically-labeled substrates and the detection of metabolite turnover in rodent brain extract samples. While many of the high-resolution applications can be labeled as routine, they are of critical importance in providing supporting information for many state-of-the-art in vivo metabolic studies, both in humans and rodents. Besides a new 500 MHz magnet and console, additional funds are requested for an auto-tunable RF probe with XYZ gradients and an automated sample changer. This combination is essential to achieve the highest productivity and system utilization by allowing hundreds of similar samples to be loaded, tuned, shimmed and measured automatically, during both night and weekend time slots. The current 500 MHz NMR system supports twelve active NIH grants, with three pending grants potentially using the system in the near future. A successful replacement of the current 500 MHz high-resolution NMR system with a new 500 MHz system will provide essential support to these NIH grants and will thereby guarantee continued productivity and innovation.

描述(由申请人提供)：通过NIH共享仪器赠款计划申请资金，用新的安捷伦500 MHz磁铁和控制台替换已有25年历史的Bruker 500 MHz磁铁和控制台。目前的高分辨率液态核磁共振系统位于耶鲁大学磁共振研究中心的地下室，该中心是耶鲁医学院首屈一指的研究建筑--安利安中心的一部分。频繁和反复的硬件故障，以及旧硬件的稳定性和同质性问题，使得在不久的将来必须更换，以保证NIH资助的大量赠款的持续生产力。目前的500 MHz系统是与活体磁共振项目密切相关的广泛生化研究的主力。这些包括但不限于，血液底物水平和13C组分浓缩的测量，新型同位素标记底物的开发，以及检测啮齿类动物脑提取液样品中代谢物的周转。虽然许多高分辨率的应用可以被贴上常规的标签，但它们在为许多最先进的体内代谢研究提供支持信息方面至关重要，无论是在人类还是啮齿动物身上。除了一个新的500 MHz磁铁和控制台外，还需要额外的资金来购买具有XYZ梯度的自动可调RF探头和自动样品转换器。这种组合对于实现最高的生产效率和系统利用率至关重要，因为它允许在夜间和周末时段自动加载、调整、填充和测量数百个类似的样本。目前的500 MHz核磁共振系统支持12个有效的NIH赠款，其中3个未决的赠款可能在不久的将来使用该系统。成功地用新的500 MHz系统取代目前的500 MHz高分辨率核磁共振系统将为NIH的这些赠款提供必要的支持，从而保证持续的生产力和创新。