Polypeptide Design with Proteomic Scope via Microfluidic mRNA Display

描述(申请人提供)：在这个项目中，我们建议将mRNA展示(一种蛋白质设计/进化方法)与高效微流控分选相结合，创造一种新技术-微流控mRNA展示-目的是能够设计出可用作蛋白质捕获试剂的多肽和蛋白质。我们将开发和应用这一强大的新技术，以创建针对丙型肝炎病毒(HCV)蛋白质组的全面试剂集。在这一部分中，我们首先描述1)基于mRNA显示的多肽和蛋白质设计，2)基于珠粒的微磁分离，以及3)介绍将成为本工作目标的丙型肝炎病毒表达的蛋白质。接下来，我们将描述我们将如何整合这些技术，以实现高通量开发新的蛋白质捕获试剂的目标。

DESCRIPTION (provided by applicant): In this project, we propose to combine mRNA display (a protein design/evolution method) and high efficiency microfluidic sorting to create a new technology-microfluidic mRNA display-for the purpose of enabling design of peptides and proteins that can be used as protein capture reagents. We will develop and apply this powerful new technology toward creating a comprehensive reagent set aimed at the Hepatitis C virus (HCV) proteome. In this section, we begin by describing the existing state-of-the-art in 1) mRNA display-based peptide and protein design, 2) bead-based micromagnetic separations, and 3) give an introduction to the proteins expressed by the HCV that will be the targets of this work. This is followed by our description of how we will integrate these technologies to achieve our goal of high-throughput development of new protein capture reagents.