Polypeptide Design with Proteomic Scope via Microfluidic mRNA Display

项目描述（由申请人提供）：在本项目中，我们提出将mRNA展示（一种蛋白质设计/进化方法）与高效微流控分选相结合，创造一种新技术——微流控mRNA展示——以设计可作为蛋白质捕获试剂的肽和蛋白质。我们将开发和应用这项强大的新技术，以创建一套针对丙型肝炎病毒（HCV）蛋白质组的综合试剂。在本节中，我们首先描述现有的最新技术：1)基于mRNA显示的肽和蛋白质设计，2)基于微磁珠的分离，以及3)介绍HCV表达的蛋白质，这些蛋白质将成为本工作的目标。接下来，我们将描述如何整合这些技术，以实现我们高通量开发新蛋白质捕获试剂的目标。

DESCRIPTION (provided by applicant): In this project, we propose to combine mRNA display (a protein design/evolution method) and high efficiency microfluidic sorting to create a new technology-microfluidic mRNA display-for the purpose of enabling design of peptides and proteins that can be used as protein capture reagents. We will develop and apply this powerful new technology toward creating a comprehensive reagent set aimed at the Hepatitis C virus (HCV) proteome. In this section, we begin by describing the existing state-of-the-art in 1) mRNA display-based peptide and protein design, 2) bead-based micromagnetic separations, and 3) give an introduction to the proteins expressed by the HCV that will be the targets of this work. This is followed by our description of how we will integrate these technologies to achieve our goal of high-throughput development of new protein capture reagents.