Polypeptide Design with Proteomic Scope via Microfluidic mRNA Display

通过微流控 mRNA 显示进行蛋白质组学多肽设计

• 批准号: 8131054
• 负责人: RICHARD W ROBERTS
• 金额: $ 48.35万
• 依托单位: UNIVERSITY OF SOUTHERN CALIFORNIA
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-30 至 2014-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8131054
• 关键词: Development; Evolution; Goals; Hepatitis C virus; Messenger RNA; Methods; Microfluidics; Peptides; Proteins; Proteome; Proteomics; Reagent; Sorting - Cell Movement; Technology; Virus; Work; base; design; new technology; polypeptide

DESCRIPTION (provided by applicant): In this project, we propose to combine mRNA display (a protein design/evolution method) and high efficiency microfluidic sorting to create a new technology-microfluidic mRNA display-for the purpose of enabling design of peptides and proteins that can be used as protein capture reagents. We will develop and apply this powerful new technology toward creating a comprehensive reagent set aimed at the Hepatitis C virus (HCV) proteome. In this section, we begin by describing the existing state-of-the-art in 1) mRNA display-based peptide and protein design, 2) bead-based micromagnetic separations, and 3) give an introduction to the proteins expressed by the HCV that will be the targets of this work. This is followed by our description of how we will integrate these technologies to achieve our goal of high-throughput development of new protein capture reagents.

描述（由申请人提供）：在该项目中，我们建议将mRNA展示（一种蛋白质设计/进化方法）和高效微流控分选相结合，创建一种新技术——微流控mRNA展示——以设计可用作蛋白质捕获试剂的肽和蛋白质。我们将开发并应用这项强大的新技术，以创建针对丙型肝炎病毒（HCV）蛋白质组的综合试剂组。在本节中，我们首先描述 1) 基于 mRNA 展示的肽和蛋白质设计、2) 基于珠子的微磁分离和 3) 介绍 HCV 表达的蛋白质（这将是本工作的目标）中现有的最先进技术。接下来是我们将如何整合这些技术来实现新蛋白质捕获试剂高通量开发的目标的描述。