MHC Typing of Macaques Used in AIDS Research

描述(申请人提供)：我们计划继续为北美和欧洲科学界提供猕猴MHC分型的服务。这将包括对编码MHC I类和II类分子的等位基因进行PCR-SSP测试，这些分子结合来自SIV的多肽。此外，我们将继续为希望在自己的实验室建立MHC打字系统的个人提供培训。最后，我们将继续开发一组具有良好特性的细胞系，这对分析猕猴体内的MHC将是无价的。到目前为止，我们已经为大约50名研究人员使用了超过11.4万种测试，输入了超过1.5万只猕猴。这些排版支持出版了75份以上的手稿。因此，这项申请的两个具体目标是：1.为研究印度恒河猴的研究人员提供I类和II类基因座的MHC分型。扩展我们的猕猴I类和II类等位基因的分子分型菜单。 公共卫生相关性(由申请人提供)：猕猴体内的SIV感染是研究艾滋病毒感染者的最佳动物模型。此外，接种疫苗的猕猴面临的SIV挑战对HIV疫苗的开发至关重要。了解猕猴的MHC分子是发病机制和疫苗研究的核心。

DESCRIPTION (provided by applicant): We plan to continue to offer services to both the North American and European scientific communities for MHC typing of macaques. This will include PCR-SSP tests for alleles encoding MHC class I and II molecules that bind peptides derived from SIV. Additionally, we will continue to offer training for individuals who wish to set up MHC typing in their own laboratories. Finally, we will continue to develop a panel of well-characterized cell lines that will be invaluable for the analysis of the MHC in the macaque. To date, we have typed more than 15,000 macaques for approximately 50 investigators using over 114,000 tests. These typings have supported the publication of over 75 manuscripts. The two specific aims of this application are, therefore, to: I. Offer MHC typing of the class I and II loci for investigators working with Indian rhesus macaques. II. Expand our menu for the molecular typing of the macaque's class I and II alleles. PUBLIC HEALTH RELEVANCE (provided by applicant): SIV infection in macaques is the best animal model to study HIV-infected humans. Furthermore, SIV challenge of vaccinated macaques is critical to HIV vaccine development. Understanding the MHC molecules of the macaque is central to both pathogenesis and vaccine studies.