Cell and Animal Model Core

在我们的研究项目中，主要从p53调节因子和效应物的体外模型中获得的见解导致了对体内验证和使用体内模型获得进一步了解的能力的迫切需要。在p53范式中被积极检测的表型不再仅仅是细胞死亡、生长停滞或衰老的问题。相反，p53在体内激活的作用可能在不同的细胞和组织类型中产生不同的结果，并且可能在发育阶段有所不同。细胞和动物模型核心（Core B）将获得和维持人类和小鼠细胞系，以及项目使用的足够的早期传代等比冷冻原代细胞。它将作为单克隆抗体和经过验证的RNAi试剂的存储库。核心还将获取和维护稳定使用的小鼠模型（转基因，敲除等）和来自这些小鼠的原代细胞，以分发给该计划的研究人员。我们的生产和合作项目的研究工作极大地受益于体内模型的组成部分，它保持了小规模的繁殖

Insights gained primarily from in vitro models of p53 regulators and effectors within our research program have led to a critical need for in vivo validation and the ability to gain further understanding using in vivo models. The phenotypes that are actively being examined in the p53 paradigm are no longer simply a matter of cell death, growth arrest or senescence. Instead, the role of p53 activation in vivo will likely have different consequences In different cell and tissue types and will likely differ with regard to developmental stages. The Cell and Animal Model Core (Core B) will derive and maintain human and mouse cell lines as well as adequate stocks of early passage aliquots of frozen primary cells utilized by the projects. It will serve as a repository for monoclonal antibodies and validated RNAi reagents. The Core will also acquire and maintain steadily used mouse models (transgenic, knockouts, etc.) and primary cells derived from these mice for distribution to the investigators in the Program. The research efforts of our productive and collaborative program greatly benefit from an in vivo models component, which maintains small breeding colonies of critically needed mouse strains for genetic and cell biologic investigations by our investigators. The high cost of animal studies in barrier facilities can be reduced substantially by the availability of shared technical support for mouse breeding and genetic analyses and for shared immunohistochemistry. The cost effectiveness is further emphasized by the shared use of the same genetically modified strains by several investigators and projects within the Program. Lastly, the core will provide expertise and guidance in the preparation of tissue and/or embryos for analyses of gene expression (protein and mRNA in situ).