PATIENT SAMPLE REPOSITORY AND SPECIALIZED FLOW CYTOMETRY

这个共享资源支持两个功能：患者样本库和专门的流式细胞仪。核心的储存库功能包括获取适当的患者样本、分离和冷冻保存单核细胞、分离和存储血浆或血清、维护注释患者样本的数据库以及向程序调查人员分发样本。我们将确保所有样本都被取消身份识别，并分配有编码的唯一识别号码，以保护患者隐私。我们还将核实我们内部监管政策的遵守情况，这些政策规定，只有签署了IRB批准的知情同意文件的患者才能获取和研究样本。核心的流式细胞术功能包括对异质样本的多参数分析和高速细胞分选，以获得定义的祖细胞群体的高度纯化的群体。 这一核心的具体目标如下： 1.确保维护病人的隐私，并核实所有样本的采集和研究都已获得知情同意。 2.确保获得项目研究人员执行的临床研究方案中指定的适当样本。 3.从患者样本中分离和冷冻单个核细胞和血浆或血清成分，以供计划研究人员后续研究。 4.维持一个载有病理、细胞遗传学和分子资料的注解资料库，以储存从髓系白血病病人取得的临床样本。 5.确保以及时和公平的方式将样本分发给实验室调查人员，以进行本申请中提议的实验。 6.提供专业的多参数分析和高速细胞分选，以获得高纯度的种群，用于功能和遗传分析

This shared resource supports 2 functions: patient sample repository and specialized flow cytometry. The repository functions of the core include the acquisition of appropriate patient samples, isolation and cryopreservation of mononuclear cells, separation and storage of plasma or serum, maintenance of a database annotating patient samples and distribution of samples to program investigators. We will safeguard patient privacy by ensuring that all samples are de-identified and are assigned coded unique identifying numbers. We will also verify compliance with our internal regulatory policies which stipulate that samples may be obtained and studied only from patients who have signed an IRB approved informed consent document. The flow cytometry functions of the core include multiparameter analysis of heterogeneous samples and high-speed cell sorting to obtain highly purified populations of defined progenitor populations. The specific aims of this core are listed below: 1. To ensure the maintenance of patient privacy and to verify that informed consent has been obtained for acquisition and study of all samples. 2. To ensure the acquisition of appropriate samples as designated in clinical research protocols carried out by program investigators. 3. To isolate and cryopreserve mononuclear cells and plasma or serum components from patient samples for subsequent study by program investigators. 4. To maintain an annotated database containing pathologic, cytogenetic and molecular information for clinical samples obtained from patients with myeloid leukemias. 5. To ensure that samples are distributed in a timely and equitable fashion to laboratory investigators for experiments proposed in this application. 6. To provide specialized multi-parameter analysis and high-speed cell sorting to obtain highly purified populations for functional and genetic analysis