Sample Processing and Immune Assessment

样品处理和免疫评估

• 批准号: 10465099
• 负责人: JEROME RITZ
• 金额: $ 31.79万
• 依托单位: DANA-FARBER CANCER INST
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-08-14 至 2024-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10465099
• 关键词: Allogenic; Antigen Targeting; Antigens; Autologous Cell Vaccine; Biological Assay; Biostatistics Core; Blood; Bone Marrow; Cells; Characteristics; Clinical; Clinical Trials; Clonal Evolution; Confidentiality of Patient Information; Cryopreservation; Cytomegalovirus; Cytometry; DNA; Dose; Enrollment; Enzyme-Linked Immunosorbent Assay; Equipment and supply inventories; Flow Cytometry; Fluorochrome; Hematopoiesis; Hematopoietic Stem Cell Transplantation; Human Herpesvirus 4; Immune; Immune response; Immunity; Immunogenetics; Immunologic Monitoring; Immunology procedure; Individual; Informed Consent; Interleukin-2; Kinetics; Laboratories; Laboratory Study; Leukemic Cell; Lymphocyte; Marrow; Measures; Metals; Minor Histocompatibility Antigens; Monitor; Monoclonal Antibodies; Mononuclear; Nivolumab; Outcome; Patient-Focused Outcomes; Patients; Peptide Vaccines; Peripheral Blood Mononuclear Cell; Phenotype; Plasma; Play; Population; Process; RNA; Regulatory T-Lymphocyte; Relapse; Research Personnel; Resources; Role; Sampling; Series; Serum; T-Lymphocyte; Therapeutic Effect; Time; Transplantation; computerized; cytokine; donor stem cell; enzyme linked immunospot assay; experimental study; immune function; immune reconstitution; immunological intervention; immunoregulation; improved; patient privacy; peripheral blood; post-transplant; programs; reconstitution; therapy design

Project Summary The purpose of Core 2 is to provide a centralized laboratory resource for processing, cryopreserving and distributing patient samples and to monitor the kinetics of immune reconstitution following allogeneic hematopoietic stem cell transplantation (HCT). Prior to transplantation, peripheral blood mononuclear cells (PBMC), bone marrow and serum or plasma are obtained from all patients enrolled on clinical trials supported by this Program Project. PBMC, plasma and serum are also obtained from normal stem cell donors. Following HCT, additional samples are obtained at regular intervals. All of these samples are processed, cryopreserved, entered into a computerized inventory and made available to Program investigators. Patient confidentiality is maintained at all levels and samples are only be obtained from patients who have provided informed consent. To support clinical trials and laboratory studies in each project, Core 2 also provides a series of phenotypic and functional immune assays that are used to monitor the effects of different immunologic interventions. Results of each of these assays are provided to Dr. Kim (Biostatistics – Core 1) for correlation with laboratory studies in each project as well as with clinical outcomes.

项目摘要 核心2的目的是提供一个集中的实验室资源， 分配患者样品并监测同种异体移植后免疫重建的动力学 造血干细胞移植（HCT）。移植前，外周血单核细胞 PBMC、骨髓和血清或血浆获自参加支持的临床试验的所有患者。 通过这个项目计划。PBMC、血浆和血清也从正常干细胞供体获得。以下 HCT，定期采集额外样本。所有这些样本都经过处理，冷冻保存， 输入计算机化库存，并提供给项目调查人员。病人保密是 维持在所有水平，仅从提供知情同意的患者中获取样本。 为了支持每个项目的临床试验和实验室研究，Core 2还提供了一系列表型和 功能性免疫测定，用于监测不同免疫干预的效果。结果 将这些试验中的每一项提供给Kim博士（生物统计学-核心1），以与 每个项目以及临床结果。