Sample Processing and Immune Assessment

项目摘要 核心2的目的是提供集中的实验室资源，用于加工、冷冻保存和 分发患者样本并监测异基因移植后免疫重建的动力学 造血干细胞移植。移植前，外周血单核细胞 (PBMC)、骨髓和血清或血浆是从所有参加支持的临床试验的患者那里获得的 由本计划项目提供。PBMC、血浆和血清也可以从正常的干细胞捐献者那里获得。跟随 对于HCT，定期采集额外的样品。所有这些样本都经过处理，冷冻保存， 进入计算机化的清单，并提供给方案调查人员。患者的保密是 维持在所有级别，样本只能从提供知情同意的患者那里获得。 为了支持每个项目的临床试验和实验室研究，Core 2还提供了一系列表型和 功能免疫分析，用于监测不同免疫干预措施的效果。结果： 其中每一项分析都提供给Kim博士(生物统计-核心1)，以便与#年的实验室研究相关联。 每个项目以及临床结果。

Project Summary The purpose of Core 2 is to provide a centralized laboratory resource for processing, cryopreserving and distributing patient samples and to monitor the kinetics of immune reconstitution following allogeneic hematopoietic stem cell transplantation (HCT). Prior to transplantation, peripheral blood mononuclear cells (PBMC), bone marrow and serum or plasma are obtained from all patients enrolled on clinical trials supported by this Program Project. PBMC, plasma and serum are also obtained from normal stem cell donors. Following HCT, additional samples are obtained at regular intervals. All of these samples are processed, cryopreserved, entered into a computerized inventory and made available to Program investigators. Patient confidentiality is maintained at all levels and samples are only be obtained from patients who have provided informed consent. To support clinical trials and laboratory studies in each project, Core 2 also provides a series of phenotypic and functional immune assays that are used to monitor the effects of different immunologic interventions. Results of each of these assays are provided to Dr. Kim (Biostatistics – Core 1) for correlation with laboratory studies in each project as well as with clinical outcomes.